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 Tanavast
Stranger
Registered: 07/20/21
Posts: 82
Loc: Roshar
Last seen: 23 days, 19 hours
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 Re: Ask Quick Questions, Get Quick Answers  [Re: LivingSpaces] 1
#27836674 - 06/25/22 07:56 PM (2 years, 2 months ago) |
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Quote:
LivingSpaces said:
For PF BRF TEK, when my jars are fully colonized, and after waiting 1 wk for consolidation, if I can't birth them right then, how long do I have before it's detrimental?
Last summer I let 2 jars go probably a month (maybe a little more) after full consolidation and they fruited. It was new genetics for me though, the flushes on both cakes were unimpressive, but like I said Iโm not sure if it was genetics or because of the longer shelf time
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 Oneleggedwarrior
Stranger
Registered: 06/12/22
Posts: 35
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27837016 - 06/26/22 04:25 AM (2 years, 2 months ago) |
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Quick question?
Have just got my ingredients and pots for starting my agar adventure, would long tweezers and a craft knife suffice for tools needed?
Thanks in advance โบ ๐๐ผ
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SwabMarley
Twisted Metal Head
Registered: 12/07/20
Posts: 1,571
Loc: Drunken Stupor
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Quote:
Oneleggedwarrior said:
Quick question?
Have just got my ingredients and pots for starting my agar adventure, would long tweezers and a craft knife suffice for tools needed?
Thanks in advance โบ ๐๐ผ
As long as you sterilise or at the very least sanitise them before use you should be okay. I used a craft knife for my first 6 months or so.
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Oneleggedwarrior
Stranger
Registered: 06/12/22
Posts: 35
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Re: Ask Quick Questions, Get Quick Answers [Re: SwabMarley]
#27837120 - 06/26/22 06:57 AM (2 years, 2 months ago) |
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Thanks for the confirmation ๐๐ผ
Will be flame sterilised as both are stainless steel ๐๐ผ
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AKAMacC
Stranger
Registered: 06/26/22
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Last seen: 2 years, 2 months
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So give it to me straight. Am I screwed? Should I throw this brick an try again or is there hopes to save?
Everything was great till my first flush. After I harvested for the first time. I soaked the bricks in distilled water for 12 hours each. It's been a week an they look like this.
For the record. Total noob here. This is my first attempt. Appreciate all the help.
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Rotnpins
๐คฎ Rotten-Pins ๐
Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
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Re: Ask Quick Questions, Get Quick Answers [Re: AKAMacC]
#27837161 - 06/26/22 08:12 AM (2 years, 2 months ago) |
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Quote:
AKAMacC said:
So give it to me straight. Am I screwed? Should I throw this brick an try again or is there hopes to save?
Everything was great till my first flush. After I harvested for the first time. I soaked the bricks in distilled water for 12 hours each. It's been a week an they look like this.
For the record. Total noob here. This is my first attempt. Appreciate all the help.
I'm not sure if they can be saved, id wait for a second opinion,. but if that picture is in your fruiting chamber you should cut back your foil. It should be barely larger than the cake, that way the perlite is able to do its job properly.
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AKAMacC
Stranger
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Re: Ask Quick Questions, Get Quick Answers [Re: Rotnpins] 2
#27837598 - 06/26/22 03:15 PM (2 years, 2 months ago) |
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Thanks Rotnpins. I'll adjust my foil. I didn't hear anything about that. I had no idea 
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Rotnpins
๐คฎ Rotten-Pins ๐
Registered: 01/11/22
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Re: Ask Quick Questions, Get Quick Answers [Re: AKAMacC] 2
#27837611 - 06/26/22 03:26 PM (2 years, 2 months ago) |
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No problem.. I'm not positive but I think that's part of your problem.. it looks to me like it's gotten dried out and matted.. but I'd wait for a 2nd opinion to make sure..
My recommendation would be to start your next project right away... it helps to keep a constant rotation of new projects while learning. That way, if things do go down hill on a project, you're able to just toss that project and not worry too much. Its a lot better than having to toss your only project, which really sucks.
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Smellyhobbit
Actual Retard
Registered: 04/01/22
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 1
#27837651 - 06/26/22 04:05 PM (2 years, 2 months ago) |
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Itโs a shame. That looks like it had an excellent pin set.
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Sockadin
Registered: 01/03/10
Posts: 7,248
Last seen: 3 months, 6 days
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Re: Ask Quick Questions, Get Quick Answers [Re: AKAMacC]
#27838439 - 06/27/22 06:11 AM (2 years, 2 months ago) |
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Yeah you can try and save them. Like rotpin said cut the foil down and raise your RH by misting the walls of the tub heavily and probably watering you perlite.
It looks like a good pin set and the nasty looking cake parts seem like bruising and matting because the cakes dried out.
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Rotnpins
๐คฎ Rotten-Pins ๐
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27838492 - 06/27/22 07:13 AM (2 years, 2 months ago) |
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Question: do you always dip your swabs in sterilized H2O before stabbing plates, or do you just do that with older spores?
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DERRAYLD
Constructus
Registered: 05/13/02
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Loc: South Africa
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Re: Ask Quick Questions, Get Quick Answers [Re: Rotnpins] 2
#27838495 - 06/27/22 07:15 AM (2 years, 2 months ago) |
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I only hydrate older spores, swabs are streaked and stabbed.
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CowsPoopShrooms
StillUphill
Registered: 06/08/22
Posts: 505
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27839216 - 06/27/22 04:21 PM (2 years, 2 months ago) |
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?
Edited by CowsPoopShrooms (10/14/22 02:03 PM)
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Bobbins
Stranger
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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] 1
#27840089 - 06/28/22 03:34 AM (2 years, 2 months ago) |
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Quote:
CowsPoopShrooms said:
.
.
Quick Question: Ok, I'm hoping this is a quick Question/Answer, How do I tell if my agar plate(s) are finally "clean"? Is it by the growth/presence of a monoculture or what? Sorry, but I don't think I've found an actual explanation at a nOOb level. I'm on T3 and getting ready to do T4s. I have 1 that is very close, IMO, to a "monoculture". What am I looking for?
I seriously doubt you have a monoculture, just nice uniform growth is what you're after, rhizo is popular because it's easier to see there's no intertwined nasties, and usually means it's quite vigorous.
--------------------
DeALeRsHrOoMs
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DERRAYLD
Constructus
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Re: Ask Quick Questions, Get Quick Answers [Re: Bobbins] 1
#27840132 - 06/28/22 04:57 AM (2 years, 2 months ago) |
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Uniformity is key when working with agar.
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Hindsight
Mad Scientist
Registered: 01/24/21
Posts: 2,706
Last seen: 1 year, 4 months
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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] 1
#27840845 - 06/28/22 03:38 PM (2 years, 2 months ago) |
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Quote:
CowsPoopShrooms said:
.
.
Quick Question: Ok, I'm hoping this is a quick Question/Answer, How do I tell if my agar plate(s) are finally "clean"? Is it by the growth/presence of a monoculture or what? Sorry, but I don't think I've found an actual explanation at a nOOb level. I'm on T3 and getting ready to do T4s. I have 1 that is very close, IMO, to a "monoculture". What am I looking for?
Some examples......
This is a T1 from multispore. There are obviously several sectors here.... several cultures growing on the same plate. To my eyes, this looks clean, but there is some risk there - perhaps the area growing around 9:00 is contaminated by some other fungus. Again, it's probably clean but some people (including myself if I've already tried growing a variety out and had contamination issues) might take more transfers until they don't see sectoring like this:
This is someone else's photo. I post this here to give an example of bacterial contamination. You can see it on the bottom. Note the sharp edges and yellowish color vs the fuzzy edges and whiter color of the mycelium above. Bacterial contamination is often round.
Bacterial contamination again. Notice the round spots with sharp edges:
Another bacterial, with some mycelium growth mixed in:
More bacteria:
You want to avoid anything of an odd color too... small black, brown, green, etc spots are contamination. I don't have any pics of that handy.
Like the first example, this is too uneven for me. I wouldn't send this:
Here are some examples of nice looking plates:
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CowsPoopShrooms
StillUphill
Registered: 06/08/22
Posts: 505
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Re: Ask Quick Questions, Get Quick Answers [Re: Hindsight]
#27840894 - 06/28/22 04:09 PM (2 years, 2 months ago) |
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Some examples......
Edited by CowsPoopShrooms (10/14/22 02:04 PM)
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Hindsight
Mad Scientist
Registered: 01/24/21
Posts: 2,706
Last seen: 1 year, 4 months
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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] 1
#27840932 - 06/28/22 04:41 PM (2 years, 2 months ago) |
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"Ok, so would those be "monocultures"? Or, just good clean even growth?"
When most people say "monoculture", they simply mean "even, consistent looking growth." A true monoculture is one dikaryotic connection between two spores. You can't take transfer after transfer and be positive that you only have ended up with one true monoculture there. But unless you are trying to grow crosses, it doesn't matter. You don't need a monoculture. You just transfer until you have nice, even, fast growth. Just remember that transferring is a double edged sword - you don't know if the culture you end up with is going to fruit, or fruit well. The time and energy you spend transferring could be wasted if what you end up with is a culture that never fruits or fruits poorly. This is why I usually err on the side slight contamination risk..... I would send plate #1 from my first post to grain, which is a T1. I rarely go beyond T1 unless I see really ugly looking growth like plate #6, which I would not send to grain. I want the widest genetics possible in hopes of stumbling on something good or unique and then cloning/printing it, understanding that I have a bit more risk of contamination (always fungal in my case since bacteria is so easy to spot).
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CowsPoopShrooms
StillUphill
Registered: 06/08/22
Posts: 505
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Re: Ask Quick Questions, Get Quick Answers [Re: Hindsight] 1
#27841187 - 06/28/22 06:57 PM (2 years, 2 months ago) |
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Edited by CowsPoopShrooms (10/14/22 02:05 PM)
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Oneleggedwarrior
Stranger
Registered: 06/12/22
Posts: 35
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 2
#27841855 - 06/29/22 05:40 AM (2 years, 2 months ago) |
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Quick question...
How long is agar (no pour method) ok to leave before inoculation and are they ok stored in cupboard?
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