In Over My Head Agar,
a novices continued adventure into the world of growing mediums.
DISCLAIMER: i understand most the pictures have been removed as i either needed space before i committed to subbing to the shroomery or they had pictures of my fingerprints in them. Im sorry but the info and sentiment to my first (really second) real glog is still here.
Welcome to second glog featuring me (some human) who got very excited to start agar again correctly and didn't realize 4-5 projects turns into a lot of work for someone who's just starting to work with petris and achieve a clean culture. Shout out to C10 for pushing me into the real part of this hobby on my last glog. My goal for this is spores to fruits with each strain so its going to be a lengthy glog. I should've probably split it into different ones but then there's no way I'm keeping up! But none the less I'm a persistent bastard when I'm intrigued with something so here we go.
The main post is less detailed and more of to serve as a quick writeup. More in depth details and individual updates/thoughts in the replies and notes at the bottom as I'm trying to keep the main post from being gigantic So the easiest way to do this is going to be sections so here they are. Running Projects (Updated 2/5): AA+, APE, TC, Mel, LPEU (Clone), RW On Hold or Cut: LPEU Both PE/T Clones Albino A+ (AA+)I started an AA+ germ plate set on some super expensive/terrible Rubbermaid mini dishes for agar just to get going before I had my petris ready. They did the job and I got a couple of transfers from AA+ Germ plate to Petris
Germination Plates: Trash Rubbermaid sorry for the pics. I honestly just spore dumped these. I tried to do some streaking but those containers suck and I remember just pouring them in and tanking what grew fastest and nicest.


Also all the holes in the petris are from
D3's Agar tek where you use a leather hole punch.
T1:

T2: 11/25


T3 12/3

12/7


T4

1/2 sent 2 plates to 4x grain jars

2/4 spawned 1 jar to 7 muda bottles inside a still air box g2g style. I made the muda bottles way too full so i couldn't put more grain in them. Im expecting contamination as the jar didn't look 100% (was tipsy and off my game in the SAB on innoc day...) and i put so little spawn in each bottle. I then put the rest of the grain that didn't fit in the 6 bottles and made a novelty grow out of one of my ceramic pieces i made in college.

Im expecting good things out of this one

The other 3 jars looked quite fucked. They are from 1/2's SAB session so not good. Only jars that look bad though!
Treasure Coast (TC) Again was impatient on the petris and started this through a ship in what would otherwise be used as some sort of LI jar I made, decided it was going to be a germ plate though and man did it get the job done.
Germ: Noticing the way myc avoids the contamination is interesting.

T1 Transfered some of the best looking colonies off the plate a couple of days ago:


T2:


T3 12/7

Albino PE (Ape)Im really excited to get this one fruiting not only for its renowned potency but I mean come on. Little white dicks that bruise blue, that's just cute as heck

I absolutely refused to wait for a germ plate to grow something as Ive heard it takes quite a bit for APE to germinate and to germinate something worth while i wasn't about to dick around, or was i? I decided that instead of using agar for germ plates... why not some prepped grain? Just kidding i did use grain to do this though as its what i had healthy growth on at the moment (waste of resources but saved on SOME time

) so here's some grain jar colonies on agar of APE. The look good!
Germ:



T1:
PE/T ClonesWhen I started up again this year, two-quart jars of agar to grain spawn followed me from my last operation a year ago with some spore i revived at the beginning of the lockdown. I spawned them after being well colonized and took clones of the monsters and some clusters that came. Here is their journey. The clones are probably a bust as I didn't know what to look for I was just like "hey a small cluster" chop chop drop drop. God those plates were so slimy. The C+ (chunk clone) is the easily second gen looking fruit that came up. Hopefully that one will be nice to carry on from.
Germ:
C+ or the chunky clone came from this fruit

you can see her surgery scars.


Is what the biopsy provided
T1:

Some aggressive recovery if u ask my novice ass.

12/2


12/7


Cloned Cluster from PET (Scraped) This culture is slow and nothing special, I've decided it doesn't make the cut

bye
no more agar for you sir.
Germ:


Love to see it going up the walls, idk its just my thing

T1:


T2

12/3
RustyWhyte(RW) Germ:


T1:





Very weird cultures in my eyes
T2:
T3:
Melmac(Mel) Spontaneously obtained a Mel clone plate and expanded it to 6 or so dishes. Pics soon
"Germ":

T1: 12/3


Heres the mel stack not posting all pics

12/7

T2:

4xT2 (really t4) from clone sent to grain 1/1






5x grain jars sent to a pastys ez dialed monotub 1 brick coir 4 quarts boiled water 2 quarts verm bucket tekt 2/1



2/5
Monotub


I wont lie i tried to do a psuedo casing with CV but didn't have enough left so the spots the myc isn't pushing through yeah that's a little bit of a casing, I dont really care enough to case any more of it i think it'll be fine. Gunna have a weird first flush i think. that's ok.








1300+g wet
Shoebox 300+g wet
Leucistic Penis Envy Uncut (LPEU)Spontaneously obtained a LPEU plate with a monster dish pin

took a biopsy from it as well as a few transfers.
"Germ":

T1: 12/3

12/7

T2:

T2 from Dish clone so really like t7 or something ridiculous sent to grain 1/1


2/4 4xjars spawned to shoeboxes 1:2 spawn:CV roughly following a few guides but mostly
Munch style.


Notes:
12/7 Keeping up to date and adding photos today. Making lots of transfers soon

We cut out the cluster clone today since it wasn't looking too good compared to some of these beauties. Unlucky buds, sorry. You win some ya lose some but at least I got some experience taking biopsies. Waiting for more dish pins

and my MS -> grain jars to hurry up and finish so I can get my quart jars back and start prepping my grains for these plates
12/10
I went ahead and expanded that AA+ culture to 6 plates today as well as pulled some nice looking colonies from my RW germ plates ill be posting up here in a few. For two of them I used my 21G needles that showed up in the mail to make a
Josex inspired Needle transfer but instead of going to a brf jar I just used it to transfer to another petri hoping to narrow down genetics and any possible contams. Tried it with a bigger needle with success looking forward to seeing the thinner needles results.
Time to prep more agar plates (a few of my dishes I poured got mold in the stack. I think I accidentally lifted them when I was wrapping them in a stack... wont do that again) and go to the store and buy a ton of shoeboxes for my MS Syringe -> grain jars that are coming around the mountain.

Thats all for today.

1/2/21
Second round of A->Grain jars made up today (AA+ and TC pints). Everything marked 1/2 could be fucked my sterile technique was off point today. Lots of rim shots on the quart rims, AC was on for half the time, fumbling with wedges going to transfer as I get a tech that works for me figured out, and a little tipsy

. So if i get some contams here it was def technique. Otehr than that... nothing to update on. Unloading some more grain jars to get pregnant and Going to do a round of agar transfers soon for ape and RW.
2/2
We are nearing the end of this log as MEL goes into a monotub and LPEU TC and AA+ are ready for shoeboxes. Ape is taking its time on the grains (who would've guessed). Thats all the cultures i want to fruit out of this grow log. I dropped RW cause its taking a lot more work then expected to get clean looking growth. Pasty himself said it was very tomentose variety and to be honest i haven't been very diligent with that culture. Only on T3. As i start 3 new varieties today my attention will go to those as i start a new log but will keep this one updated as the tubs fruit out. <3
2/4
Working on my diligence (also waiting on new projects to germinate) were taking RW off hold.
I went back to the germination plates with it since all my transfers look pretty thin. If i get the same results here ill just send them to grain and clone hunt as i do remember Pasty saying the culture on agar was not consistent with other myc... I think.
Also took a bunch of dish pin clones from r.Melmac and my PE/T C+ clones. So now i have PE/T C+ C+ lmfao and i hope it gets an A at fruiting time. God that was a terrible joke

3/4
Im nearin the end of this Glog as i have fruit in hand for all strains except AA+ (spawn jars went bacterial and the ones that didn't went to MudaBottles that are taking FOREVER) and TC which i expanded via G2G and will have in monotubs shortly. And RW... that diligence thing. I need to order more petris and get cracking on some no pours 2.