Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: < First | | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Re: Making Liquid Culture [Re: ChardRich] * 1
    #26280538 -

This is pan cyan at 5 days. Jar is almost full of myc. Little gap at the top.
It is super-dense and broken up, ready to go directly into a syringe.



--------------------
Cultivating Panaeolus cyanescens


Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: brainwave]
    #26280597 -

brainwave said:
I have been looking at beer LC seems interesting. You always have beer around it seems. Any particular beer?  Silly question but do you think you can use light beer or is the calories needed?



Idk if light beer will make a difference. I looked it up recently bc I'm trying to get my six pack (abs not beer) and it says the difference in calories is mostly from a decrease in alcohol concentration. Alcohol becomes sugars which are calories. So ppl get light beer and then have to drink more of it and take in more calories than if they just drank regular beer. That being said idk. I used 50 ml beer, so theres 2.5 ml alcohol. When I use it on agar I still can get bacteria so I imagine the alcohol is lost in sterilization.

This was 50/50 Miller/water. I wouldn't call it a Tek, it's just something I did bc it was 3am and there was room in the PC. Mostly I like beer for agar. Just something new to stare at while jars colonize.
:mygoditsfullofstars:


--------------------


Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:



Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: spiritlands]
    #26280643 -

Asura, How'd you noc her up? How much inoculate

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: verum subsequentis]
    #26280671 -

verum subsequentis said:
Asura, How'd you noc her up? How much inoculate



I usually tic-tac-toe a plate in there. Usually about 6 small wedges. It depends.
I'm using a fresh blade and a sterilized handle. I'll cut up the plate and whatever
I can scoop up in one go makes it to the jar. I don't recommend this...but it's
what I'm doing.

Edit: for cubes I'd just to a single wedge or even Josex poke.


--------------------
Cultivating Panaeolus cyanescens


Edited by Asura (10/27/19 04:56 PM)

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Asura]
    #26280717 -

Asura said:
verum subsequentis said:
Asura, How'd you noc her up? How much inoculate



I usually tic-tac-toe a plate in there. Usually about 6 small wedges. It depends.
I'm using a fresh blade and a sterilized handle. I'll cut up the plate and whatever
I can scoop up in one go makes it to the jar. I don't recommend this...but it's
what I'm doing.



So you inoculate 6 small wedges into a single quart mason jar?

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Mr. Alien]
    #26280723 -

Yeah


--------------------
Cultivating Panaeolus cyanescens


Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Asura]
    #26280788 -

Asura said:




Awesome! that thick growth in 5 days :O.
With only one wedge how long it would
take to achieve these results?

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Mr. Alien]
    #26280794 -

It can be just as fast. Maybe not as thick as that pic, but usable.

I only do the multiple wedges for cyans because I want as much diversity
in the LC as possible.


--------------------
Cultivating Panaeolus cyanescens


Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Asura]
    #26280868 -

Asura said:
It can be just as fast. Maybe not as thick as that pic, but usable.

I only do the multiple wedges for cyans because I want as much diversity
in the LC as possible.



:takingnotes:

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Mr. Alien] * 1
    #26281082 -

bought these syringe filters for LC

https://www.amazon.com/gp/product/B07PJV1LBY/ref=ppx_yo_dt_b_asin_title_o00_s00?ie=UTF8&psc=1

Will they melt on the autoclave at 15 psi?

Edit: I figured this is a pour thread. Sorry OP. :facepalm:

Edit 2: https://www.amazon.com/Microlit-SCIT-60-Bottletop-Dispenser/dp/B07C1VWV1S/ref=sr_1_5?keywords=Dispensette%2Bpp&qid=1572229750&sr=8-5&th=1

What do you guys think about that autoclavable dispenser than can be adapted to media bottles? Seems like the holy grail for pouring :eek:.

Edited by Mr. Alien (10/27/19 09:17 PM)

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Mr. Alien]
    #26283457 -

Hah, haha.... i think theyre really proud of that dispenser


--------------------
MyLAGM2020Growlog

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Cootermonkey]
    #26283463 -

ill just pour lc im too stoneed to really understand why id need that:stoned:

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: mushboy]
    #26283599 -

I use media and snapple bottles for LC. Pop the top off and pour, easy and environmentally conscious what with the reusing you kno.

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: mushboy]
    #26283610 -

mushboy said:
ill just pour lc im too stoneed to really understand why id need that:stoned:



:lol:

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Mr. Alien]
    #26283766 -

Ive mixed cold and just cant seem to get a solution that is clear enough.  Ive tried the mix it cold and put it in the fridge.  I take it there will always be some sediment.  Im using LME.  What strength do you use grain water at, do you dilute at all?

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Edmunter]
    #26283782 -

Have yall ever used the mini miller high life bottles? Its like 4 bucks a six pack of em at dollar general, holds 7 fluid ounces i believe.
Im planning on trying it in due time, just curious if id have to get em a silicone cap or somethin for the pc cycle.


--------------------
MyLAGM2020Growlog

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Edmunter]
    #26283812 -

Edmunter said:
Ive mixed cold and just cant seem to get a solution that is clear enough.  Ive tried the mix it cold and put it in the fridge.  I take it there will always be some sediment.  Im using LME.  What strength do you use grain water at, do you dilute at all?



I just use tap water with LME, it works well enough :shrug:

1 gram to 500mL


--------------------

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: sh4d0ws]
    #26283894 -

@mr alien:  that thing looks like a breast milk pump or something. :facepalm3:  what mushboy said about being stoned and stuff or whatever...

yeah, I do .5g to 500ml works good  but its a rough 0.5... I barely ever measure anymore


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: TedsDead]
    #26284032 -

Is this ok?  LME, I would like them to be clearer but thought id ask.  This is a day after mixing in the fridge ready to PC

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Edmunter]
    #26284187 -

I don't see any pics ther Ed. I actually added some rye flour to a few LC I made. Colonized no problemo despite the cloudiness. They really liked the flour sediments, colonized it on the bottom foremost.

Extras: Filter Print Post Top
Jump to top Pages: < First | | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | | Last >

Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy


Similar ThreadsPosterViewsRepliesLast post
* 1st Liquid Culture, arrrgh! Seril 15,587 10 02/28/06 08:35 PM
by Limelight_Liver
* Starting a simple liquid culture method Garret_Hill 9,978 3 03/11/02 09:49 AM
by YourHighness
* Easy Liquid Culture Container Tek SixTango 21,764 7 01/17/12 04:59 PM
by dubyastep
* Liquid Cultures to Bulk Substrate Prodijal_Son 19,199 7 05/05/20 03:40 AM
by poisoned
* liquid culture failure gray1 6,483 3 07/19/01 09:37 PM
by Azure
* liquid culture using h2o2 baja 9,954 14 03/09/17 10:00 PM
by 3B3B4E
* Liquid culture inoculation of straw... possible? The FUnGal FiEnD 7,983 9 05/26/13 04:57 PM
by cronicr
* Liquid culture for alkaloids?? Dr. Slavic 973 3 01/26/03 06:29 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
95,352 topic views. 11 members, 858 guests and 98 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.01 seconds on 16 queries.