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OfflineChardRich
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Registered: 04/16/19
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Re: Making Liquid Culture [Re: spiritlands]
    #26280395 - 10/27/19 02:43 PM (4 years, 3 months ago)

Your LC will look different when using a stir plate. It will pool up all on the bottom instead of looking like Mush's op and my post above. Just gotta shake before using when it's ready to get in swirling.


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OfflineAsuraS
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Re: Making Liquid Culture [Re: spiritlands]
    #26280425 - 10/27/19 02:58 PM (4 years, 3 months ago)

Quote:

spiritlands said:
My stir plate came in and my last practice lc doesn't look that cool. It's only been a week though and I can only shake it. It was Karo lc. There's stuff in there but the Mycelium I transferred doesn't look like it's growing colony balls yet. The Karo has a huge opening on the bottle and I think there's to much nutes. I used a quarter size drop in a half pint jar.

This new lc is 50/50 beer and water. Transferred Redboy on agar into it last night and it's on the stirplate. I have lme somewhere but I prob need to get fresh stuff, I just like the convenience of beer when I make agar, Mycelium likes it. This is mostly just an experiment and to play with my stirplate until fresh pan cyan prints arrive.

Can't upload pics, bought a supporter account last night but it hasn't upgraded my profile yet...




Karo by itself doesn't work very well in my experience. When I first started doing LC's years ago, I did Roadkills LC recipe which was just Karo. Took forever
to grow out, but I had success with it. I recently tried this again just to see...and it sucks pretty much. LME is the way to go.


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Offlinebottle
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Re: Making Liquid Culture [Re: Asura]
    #26280445 - 10/27/19 03:07 PM (4 years, 3 months ago)

not bashing this thread in any way. just curious why in the original thread you stated " just take my word and DONT MAKE LCs WITH SPORES." whats the reasoning behind this? Ive never had issues/failures with making lc with spores. also, i dont pour my lc because i dont have any kind of hepa filter/flow hood set up but, i probly should cuz that seems so much easier than using a syringe every time i need to inoculate hah


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OfflineChardRich
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Re: Making Liquid Culture [Re: bottle]
    #26280450 - 10/27/19 03:09 PM (4 years, 3 months ago)

Because syringes aren't inherently clean, and LC requires you to be as clean as possible. You can pour inside an SAB just fine.


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Edited by ChardRich (10/27/19 03:11 PM)


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Offlinebottle
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Re: Making Liquid Culture [Re: ChardRich]
    #26280459 - 10/27/19 03:12 PM (4 years, 3 months ago)

you mean the spore solution or the syringe itself? honestly, i prefer using large spawn bags that are sealed before pc'ing anyway. so i guess having to use syringes instead of pouring seems easier in my situation.


Edited by bottle (10/27/19 03:16 PM)


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: bottle]
    #26280465 - 10/27/19 03:14 PM (4 years, 3 months ago)

the spore solution.


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OfflineChardRich
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Re: Making Liquid Culture [Re: bottle]
    #26280468 - 10/27/19 03:14 PM (4 years, 3 months ago)

The spore solution in the syringe. I guess the needle could be dirty too tho


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Offlinebottle
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Re: Making Liquid Culture [Re: ChardRich]
    #26280476 - 10/27/19 03:19 PM (4 years, 3 months ago)

gotcha. i guess ive just been lucky. also, one clean spore syringe can produce a magnitude of lc so, ive never had to use them often. if i ever get a contam in the future, ill be sure to blame it on the spores!! hah


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OfflineChardRich
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Re: Making Liquid Culture [Re: bottle]
    #26280480 - 10/27/19 03:22 PM (4 years, 3 months ago)

You also have the added benefit of being able to use isolated genes or clones with agar


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Offlinespiritlands
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Re: Making Liquid Culture [Re: Asura]
    #26280481 - 10/27/19 03:22 PM (4 years, 3 months ago)

Ya I think Karo sucks too. When I do this for pans I'll be following your Tek in every detail asura.

I don't know much about beer brewing but I know that mold loves residue from spilled beer. I know lme and other malt extracts contribute to the flavor and color of beer. This has been my reasoning for substituting it so much. And I like to sip some suds while I work.

I've been wondering if its important to wait for the LC to colonize/consume all the nutes before using or just use when enough tissue has grown? And I'm running my plate just fast enough to make a vortex but not enough to hear noise.


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Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




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Offlinebrainwave
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Re: Making Liquid Culture [Re: spiritlands]
    #26280485 - 10/27/19 03:25 PM (4 years, 3 months ago)

I have been looking at beer LC seems interesting. You always have beer around it seems. Any particular beer?  Silly question but do you think you can use light beer or is the calories needed?


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Bio Neural


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OfflineAsuraS
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Re: Making Liquid Culture [Re: spiritlands]
    #26280489 - 10/27/19 03:26 PM (4 years, 3 months ago)

Well I do use a little Karo with the LME in my LC, but I wanted to see what was doing the heavy
lifting. LME is definitely doing the heavy lifting.


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Offlinebottle
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Re: Making Liquid Culture [Re: spiritlands]
    #26280491 - 10/27/19 03:27 PM (4 years, 3 months ago)

just my opinion but, i dont think karo "sucks" it works totally fine. the only difference ive noticed is that with malt/agar, the mycelium slurry or "broth" tends to be a bit thicker with growth. but karo in no way produces an inadequate lc


Edited by bottle (10/27/19 03:30 PM)


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OfflineAsuraS
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Re: Making Liquid Culture [Re: bottle] * 1
    #26280497 - 10/27/19 03:31 PM (4 years, 3 months ago)

My LC's are fully ready to use in 5 days. And if it takes longer
than that, it's not optimal. The growth in my Karo only jars, compared
to my normal results, did in fact, suck quite hard.


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Invisiblenatedawgnow
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Re: Making Liquid Culture [Re: Asura]
    #26280505 - 10/27/19 03:34 PM (4 years, 3 months ago)

Ready to use in 5 days total? Holy crap that's fast. I see ready to use lcs in just under
2 weeks, but I also dont use a stir plate so I don't know if the difference is that huge


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Offlinebottle
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Re: Making Liquid Culture [Re: Asura]
    #26280507 - 10/27/19 03:35 PM (4 years, 3 months ago)

understandable. when i use fresh tissue to lc with karo, it still takes roughly 10 days untill "complete." if time is the factor, then yes, karo sucks in comparison.


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OfflineAsuraS
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Re: Making Liquid Culture [Re: natedawgnow]
    #26280512 - 10/27/19 03:36 PM (4 years, 3 months ago)

Nate, if you are using LC on the regular, a stir plate is a must. Do yourself
a favor and pick up a cheap one. The difference is night and day.


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Offlinebottle
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Re: Making Liquid Culture [Re: Asura]
    #26280515 - 10/27/19 03:37 PM (4 years, 3 months ago)

does stir plate help with density of growth, just time or both?


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OfflineChardRich
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Re: Making Liquid Culture [Re: bottle]
    #26280520 - 10/27/19 03:39 PM (4 years, 3 months ago)

Quote:

bottle said:
does stir plate help with density of growth, just time or both?




both


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OfflineEdmunter
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Re: Making Liquid Culture [Re: ChardRich]
    #26280535 - 10/27/19 03:42 PM (4 years, 3 months ago)

Is this too murky?




Its in the fridge atm for tomorrow fry up


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