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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Cultivation Supplies   Mushroom-Hut Liquid Cultures

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InvisibleSunnyDayze
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Re: Making Liquid Culture [Re: YogiBear]
    #26165326 - 09/02/19 01:01 PM (4 years, 4 months ago)

Quote:

YogiBear said:
Anyone can reply to this if you know the answer...

Is this the LME that I need to get?

Thanks for any and all replys.







Looks right to me. I think any light malt extract will do :shrug:

Love your signature, glad to see you are exercising your new found freedom there in Denver! :cheers:


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OfflineTweeq
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Re: Making Liquid Culture [Re: mushboy]
    #26165338 - 09/02/19 01:10 PM (4 years, 4 months ago)

Quote:

mushboy said:
I personally test my lc on grains:blush:

But as a matter of good practice you should always test on agar:cop2:




This exactly

I don't pour my own agar petris, Just buy pre made ones for like 60cts a dish. That makes testing on grain cheaper than on agar. That's my excuse for not testing on agar. Everyone seems to have one..

Repost in the confessions thread? :smile:


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: Tweeq]
    #26165373 - 09/02/19 01:29 PM (4 years, 4 months ago)

Mush, do you test on a bunch of grain or a single jar? I would skip the agar if i wasn't trying to throw my LC into 120 pounds of wbs every time. It takes me just a few minutes to streak a plate and then I know that the bags will be fine.


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Invisiblenix21
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26165540 - 09/02/19 03:44 PM (4 years, 4 months ago)

Do you guys swirl LC every few jars so you dont end up with myc on bottom after 4 or 5 jars? Or is it fine to just swirl once before pouring?


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: verum subsequentis] * 1
    #26165545 - 09/02/19 03:51 PM (4 years, 4 months ago)

if i were making 120lbs of wbs id test on agar like 10 times:lol::paranoid:

but a small batch of LC ill just test on a single jar. seal up the lc just like i would agar and use when confident the inoculate is clean.

i suppose during that single pour i could contam the lc but the same could be said about syringes and why add a step:shrug:


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: mushboy]
    #26165561 - 09/02/19 04:00 PM (4 years, 4 months ago)

I feel ya man.

Nix, I swirl right before pouring each jar.


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26165568 - 09/02/19 04:03 PM (4 years, 4 months ago)

yah swirl that shit:naughty:


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Offlineprimelines
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Re: Making Liquid Culture [Re: mushboy]
    #26165616 - 09/02/19 04:39 PM (4 years, 4 months ago)

I poured half a colonized 250ml media bottle into 9 jars 3 nights ago. I came back and only half the jars show a tiny bit of nice looking growth.
Is that normal or very slow? I hope I swirled enough before each pour.


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OfflineFailboat
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Re: Making Liquid Culture [Re: primelines]
    #26165641 - 09/02/19 04:55 PM (4 years, 4 months ago)

Depends on how colonized the LC really was, but that sounds similar to my results just recently. This has been my first confident use of LC and I even managed to dirty a jar with my technique.


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InvisibleYogiBear
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Re: Making Liquid Culture [Re: primelines] * 1
    #26165809 - 09/02/19 06:53 PM (4 years, 4 months ago)

Quote:

primelines said:
I poured half a colonized 250ml media bottle into 9 jars 3 nights ago. I came back and only half the jars show a tiny bit of nice looking growth.
Is that normal or very slow? I hope I swirled enough before each pour.




I have a hanna magnetic stirrer that is the bomb. Well worth the money. I use to use it for extracting DMT and mescaline from plants and different cactus. Learned that on the DMT Nexus years ago. Glad I kept this piece of equipment.


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Offlineprimelines
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Re: Making Liquid Culture [Re: YogiBear]
    #26165864 - 09/02/19 07:28 PM (4 years, 4 months ago)

I meant I poured 125 ml lc into 9 grain jars. Should have clarified that, but I do want a stir bar eventually. How long do you stir lc each time?


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: primelines]
    #26165923 - 09/02/19 08:12 PM (4 years, 4 months ago)

i just do a quick swirl to ensure even distribution of the myc.


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OfflineFailboat
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26167234 - 09/03/19 04:14 PM (4 years, 4 months ago)

So I was going to order more media bottles to ramp up my LC efforts, but they're either cheap and shitty or pricey. I have 6 glass snapple bottles I'm bouta whip up some broths. Hopefully they will last a few batches.


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: Failboat] * 1
    #26167550 - 09/03/19 07:14 PM (4 years, 4 months ago)

Ive used gourmet salad dressing jars. Marzetti brand.


Lids are 60mm and pp5:awedance:



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InvisibleMycolorado
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Re: Making Liquid Culture [Re: mushboy]
    #26167555 - 09/03/19 07:17 PM (4 years, 4 months ago)

That’s some real gourmet shit.  Classy.


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Offlineprimelines
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Re: Making Liquid Culture [Re: mushboy]
    #26167573 - 09/03/19 07:23 PM (4 years, 4 months ago)


Pp5 is life

Also not wanting to encourage laziness. My  sterile technique is adequate but not bulletproof by any means. I followed this tek to a t and didnt test on agar.  Nocd 10 jars and all are great. A bit slow cuz I didn't swirl enough before pouring but I'm just saying If you follow this and have a decent culture and sab you are golden. Thanks for getting me on lc mush :smile:


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Edited by primelines (09/03/19 07:28 PM)


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Offlinebongoman
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Re: Making Liquid Culture [Re: primelines]
    #26167595 - 09/03/19 07:38 PM (4 years, 4 months ago)

Quote:

primelines said:

Pp5 is life




I keep trying out PP5 containers for various aspects of mush cult and want to like it but find the diminished visibility annoying.


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Offlineprimelines
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Re: Making Liquid Culture [Re: bongoman]
    #26167607 - 09/03/19 07:43 PM (4 years, 4 months ago)

I've figured how to keep condensation down and when I shine a strong light I can see good.


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: primelines]
    #26167644 - 09/03/19 07:58 PM (4 years, 4 months ago)

Quote:

primelines said:
Thanks for getting me on lc mush :smile:




:thaaannks:


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Offlinesan pedro guy
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Re: Making Liquid Culture [Re: mushboy]
    #26171536 - 09/05/19 09:52 PM (4 years, 4 months ago)

Got me some LC goin, first timer here.



Not sure how important the lme is but I don’t have any. This broth is some diluted grain water with a single grain inoculant. Started slow but seems to be taking off!

I also tried micro batches. I am very small scale atm so I usually only do 2 quart jars of grain at a time. This was also grain water. Dumped this into two quart jars.




Does it matter if I shake the grains up after the pour? It had a nice little pool at the bottom so I shook but could I just let it soak?

Thanks mushboy, you’re crazy but I like you


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Noob Grow Along 2022


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