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Re: Making Liquid Culture [Re: Asura]
    #26141347 -

Asura said:
I don't know if the dextrose is doing the heavy lifting, though. As much as people like to shit on it, I've had too much success with it to
take it out.


I've just purchased this, it has added dextrose. Does anyone have experience using this kind of malt extract?



Initially it mixed together fine in the cold water but produced a lot of sediment post cycle:




Edited by Stipe-n Cap (08/18/19 01:44 PM)

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Re: Making Liquid Culture [Re: verum subsequentis]
    #26142557 -

verum subsequentis said:
So, we'll see how and if colonization times differ but it appears that i think what i think because i use more malt. If i do it my way I can avoid all the chunky shit floating around. But really, that shit isn't a problem. It'll get gobbled up just the same.


Super excited for this. Thanks for taking the time to run the experiement AND post side-by-side pictures. I'm still not confident enough to do anything side by side like this, still working on getting above 90% success rate, and holding off until then.

p9hu7 said:
Initially it mixed together fine in the cold water but produced a lot of sediment post cycle:




Looks like it may have caramelized in the PC? What was your PC time? Dextrose is going to make it easier to caramelize, I think that's why Asura cuts down the PC time so hard.


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Re: Making Liquid Culture [Re: ShaperDreaming]
    #26142570 -

15 mins, but the gauge crept up to 19 psi when I walked away :shrug:

Never had a clear solution go so funky after a cycle before. I assume there are additives that fell out of solution when heated.

The health food grocery store that has LME was sold out but still had this stuff. Never used it before. Looked fine when I poured plates with it but I'll likely dump that lc and order some proper LME from Amazon.

*edit* looked at the bottom of my media bottles, full of sediment that settled while cooling. I wouldn't recommend using this product to anyone who reads this thread in the future.

Edited by Stipe-n Cap (08/19/19 09:46 AM)

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Re: Making Liquid Culture [Re: Stipe-n Cap]
    #26142598 -

p9hu7 said:
Asura said:
I don't know if the dextrose is doing the heavy lifting, though. As much as people like to shit on it, I've had too much success with it to
take it out.


I've just purchased this, it has added dextrose. Does anyone have experience using this kind of malt extract?



Initially it mixed together fine in the cold water but produced a lot of sediment post cycle:






Diastatic powder is malted baking powder used to give darker/rust colors to baked goods.

Couldnt imagine a crappier choice:shrug: live and learn.

Thanks for posting:cheers:


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Making Liquid Culture [Re: mushboy]
    #26142606 -

Lol, yeah it would appear that way. Live and learn.

I poured a sleeve of plates with it, so it will be interesting to see what happens there.

Edited by Stipe-n Cap (08/19/19 09:56 AM)

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Re: Making Liquid Culture [Re: Stipe-n Cap]
    #26142673 -

That's absolutely the kind of mistake I would make. My first agar recipe I ended up using potato water and molasses :rofl: I mean, it worked, and I bet your plates will too, but... it wasn't the prettiest thing ever.


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Re: Making Liquid Culture [Re: mushboy]
    #26142725 -

mushboy said:
p9hu7 said:
Asura said:
I don't know if the dextrose is doing the heavy lifting, though. As much as people like to shit on it, I've had too much success with it to
take it out.


I've just purchased this, it has added dextrose. Does anyone have experience using this kind of malt extract?



Initially it mixed together fine in the cold water but produced a lot of sediment post cycle:






Diastatic powder is malted baking powder used to give darker/rust colors to baked goods.

Couldnt imagine a crappier choice:shrug: live and learn.

Thanks for posting:cheers:


I bought the same thing and was told in here it'll work fine. Yes lots of sediment but shes growin good.


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Re: Making Liquid Culture [Re: primelines]
    #26142927 -

Pics:begger:


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Making Liquid Culture [Re: mushboy]
    #26143172 -

mushboy said:
Pics:begger:


Almost 4 days now, looks like it ate most of the sediment and is fairly clear when still.



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Re: Making Liquid Culture [Re: primelines]
    #26143173 -

Cool.

Sediment does get consumed and the broth will clear up. Good to know it still 'works'. I thought since it was malted it wouldnt work to great. Like trying to.use malted grains as spawn.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Making Liquid Culture [Re: mushboy]
    #26144030 -

on the 5th day...
Beer LC


When I inoculated I dropped about a centimeter squared piece. I'm honestly surprised I see this much in such short time. Mikey likes it!

I've stirred twice and I think I might use it within a few more days. What do y'all think? Based on what you see, when could I go ahead and use this?

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Re: Making Liquid Culture [Re: El Chupacabra]
    #26144466 -

Looks like you're about halfway there? Maybe a little more or less than that, hard to tell how much a 3d object is colonized on a 2d image. You could always just try to stir it up then put it to agar and if it grows you good to go :shrug:


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Re: Making Liquid Culture [Re: ShaperDreaming]
    #26144637 -

Hell yea.
I can wait a bit longer for it anyway. Thanks shaper.

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Re: Making Liquid Culture [Re: El Chupacabra]
    #26144645 -



Lost Boys quotes, fuck yeah!:rockon:

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Re: Making Liquid Culture [Re: Failboat]
    #26147410 -

Frist time doing LC, i did add cold water and 0.8-0.9g LME... LC was PCd for 45mins since i have 12psi PC..

Here is pic before and after swirling.. is it alright?


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Re: Making Liquid Culture [Re: nix21]
    #26147432 -

45 at 12psi is way to long. Even at 12 I'd still only do 15min-20min.

Should still work tho


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Making Liquid Culture [Re: mushboy]
    #26147492 -

I have a 12 psi PC that I often use to do agar in. Last time I did it for only 20 min every blank plate showed bacteria in 3 days.


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Re: Making Liquid Culture [Re: Pastywhyte]
    #26147507 -

even with a liquid?

However.. Further research does indicate 30-40min for lower psi.


edit: imma pc an lc for 20@12psi and see what it does. best way to find out is to do it yo self:awegroove:


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

Edited by mushboy (08/22/19 08:35 AM)

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Re: Making Liquid Culture [Re: mushboy]
    #26147570 -

mushboy said:
even with a liquid


Agar is a liquid :rofl:

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Re: Making Liquid Culture [Re: san pedro guy]
    #26147577 -

liquids sterilize insanely easy. like boiling water in a pot is only 212f.

so my assumption was you should be able to sterilize that liquid at 12psi in 15min because its just a liquid.


san pedro guy said:
Agar is a liquid



..well.... sorta.. agar itself is seaweed. agar as we call it is a semi firm 2d surface. the liquid or water(agar or not) is the BROTH.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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