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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Making Liquid Culture [Re: Asura]
#26141347 - 08/18/19 12:13 PM (4 years, 5 months ago) |
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Quote:
Asura said: I don't know if the dextrose is doing the heavy lifting, though. As much as people like to shit on it, I've had too much success with it to take it out.
I've just purchased this, it has added dextrose. Does anyone have experience using this kind of malt extract?

Initially it mixed together fine in the cold water but produced a lot of sediment post cycle:

Edited by Stipe-n Cap (08/18/19 01:44 PM)
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ShaperDreaming
Weirdo



Registered: 10/30/18
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Quote:
verum subsequentis said: So, we'll see how and if colonization times differ but it appears that i think what i think because i use more malt. If i do it my way I can avoid all the chunky shit floating around. But really, that shit isn't a problem. It'll get gobbled up just the same.
Super excited for this. Thanks for taking the time to run the experiement AND post side-by-side pictures. I'm still not confident enough to do anything side by side like this, still working on getting above 90% success rate, and holding off until then.
Quote:
p9hu7 said: Initially it mixed together fine in the cold water but produced a lot of sediment post cycle:

Looks like it may have caramelized in the PC? What was your PC time? Dextrose is going to make it easier to caramelize, I think that's why Asura cuts down the PC time so hard.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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15 mins, but the gauge crept up to 19 psi when I walked away
Never had a clear solution go so funky after a cycle before. I assume there are additives that fell out of solution when heated.
The health food grocery store that has LME was sold out but still had this stuff. Never used it before. Looked fine when I poured plates with it but I'll likely dump that lc and order some proper LME from Amazon.
*edit* looked at the bottom of my media bottles, full of sediment that settled while cooling. I wouldn't recommend using this product to anyone who reads this thread in the future.
Edited by Stipe-n Cap (08/19/19 09:46 AM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,263
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Quote:
p9hu7 said:
Quote:
Asura said: I don't know if the dextrose is doing the heavy lifting, though. As much as people like to shit on it, I've had too much success with it to take it out.
I've just purchased this, it has added dextrose. Does anyone have experience using this kind of malt extract?

Initially it mixed together fine in the cold water but produced a lot of sediment post cycle:

Diastatic powder is malted baking powder used to give darker/rust colors to baked goods.
Couldnt imagine a crappier choice live and learn.
Thanks for posting
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Making Liquid Culture [Re: mushboy]
#26142606 - 08/19/19 09:48 AM (4 years, 5 months ago) |
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Lol, yeah it would appear that way. Live and learn.
I poured a sleeve of plates with it, so it will be interesting to see what happens there.
Edited by Stipe-n Cap (08/19/19 09:56 AM)
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ShaperDreaming
Weirdo



Registered: 10/30/18
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That's absolutely the kind of mistake I would make. My first agar recipe I ended up using potato water and molasses I mean, it worked, and I bet your plates will too, but... it wasn't the prettiest thing ever.
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primelines
Agarth Brooks


Registered: 02/25/16
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Re: Making Liquid Culture [Re: mushboy]
#26142725 - 08/19/19 11:24 AM (4 years, 5 months ago) |
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Quote:
mushboy said:
Quote:
p9hu7 said:
Quote:
Asura said: I don't know if the dextrose is doing the heavy lifting, though. As much as people like to shit on it, I've had too much success with it to take it out.
I've just purchased this, it has added dextrose. Does anyone have experience using this kind of malt extract?

Initially it mixed together fine in the cold water but produced a lot of sediment post cycle:

Diastatic powder is malted baking powder used to give darker/rust colors to baked goods.
Couldnt imagine a crappier choice live and learn.
Thanks for posting
I bought the same thing and was told in here it'll work fine. Yes lots of sediment but shes growin good.
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mushboy
modboy



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Re: Making Liquid Culture [Re: primelines]
#26142927 - 08/19/19 01:48 PM (4 years, 5 months ago) |
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Pics
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primelines
Agarth Brooks


Registered: 02/25/16
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Re: Making Liquid Culture [Re: mushboy]
#26143172 - 08/19/19 04:23 PM (4 years, 5 months ago) |
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Quote:
mushboy said: Pics
Almost 4 days now, looks like it ate most of the sediment and is fairly clear when still.
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mushboy
modboy



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Re: Making Liquid Culture [Re: primelines]
#26143173 - 08/19/19 04:25 PM (4 years, 5 months ago) |
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Cool.
Sediment does get consumed and the broth will clear up. Good to know it still 'works'. I thought since it was malted it wouldnt work to great. Like trying to.use malted grains as spawn.
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El Chupacabra
Stoned Ape



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Re: Making Liquid Culture [Re: mushboy]
#26144030 - 08/20/19 07:05 AM (4 years, 5 months ago) |
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on the 5th day... Beer LC

When I inoculated I dropped about a centimeter squared piece. I'm honestly surprised I see this much in such short time. Mikey likes it!
I've stirred twice and I think I might use it within a few more days. What do y'all think? Based on what you see, when could I go ahead and use this?
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ShaperDreaming
Weirdo



Registered: 10/30/18
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Looks like you're about halfway there? Maybe a little more or less than that, hard to tell how much a 3d object is colonized on a 2d image. You could always just try to stir it up then put it to agar and if it grows you good to go
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El Chupacabra
Stoned Ape



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Hell yea. I can wait a bit longer for it anyway. Thanks shaper.
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Failboat
Fuck Up

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Lost Boys quotes, fuck yeah!
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nix21
Stranger



Registered: 05/29/18
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Re: Making Liquid Culture [Re: Failboat]
#26147410 - 08/22/19 06:14 AM (4 years, 5 months ago) |
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Frist time doing LC, i did add cold water and 0.8-0.9g LME... LC was PCd for 45mins since i have 12psi PC..
Here is pic before and after swirling.. is it alright?
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mushboy
modboy



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Re: Making Liquid Culture [Re: nix21]
#26147432 - 08/22/19 06:49 AM (4 years, 5 months ago) |
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45 at 12psi is way to long. Even at 12 I'd still only do 15min-20min.
Should still work tho
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
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Re: Making Liquid Culture [Re: mushboy]
#26147492 - 08/22/19 07:53 AM (4 years, 5 months ago) |
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I have a 12 psi PC that I often use to do agar in. Last time I did it for only 20 min every blank plate showed bacteria in 3 days.
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mushboy
modboy



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Re: Making Liquid Culture [Re: Pastywhyte]
#26147507 - 08/22/19 08:04 AM (4 years, 5 months ago) |
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even with a liquid?
However.. Further research does indicate 30-40min for lower psi.
edit: imma pc an lc for 20@12psi and see what it does. best way to find out is to do it yo self
Edited by mushboy (08/22/19 08:35 AM)
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san pedro guy
Captain



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Re: Making Liquid Culture [Re: mushboy]
#26147570 - 08/22/19 08:46 AM (4 years, 5 months ago) |
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Quote:
mushboy said: even with a liquid
Agar is a liquid
-------------------- Noob Grow Along 2022
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mushboy
modboy



Registered: 04/24/05
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liquids sterilize insanely easy. like boiling water in a pot is only 212f.
so my assumption was you should be able to sterilize that liquid at 12psi in 15min because its just a liquid.
Quote:
san pedro guy said: Agar is a liquid
..well.... sorta.. agar itself is seaweed. agar as we call it is a semi firm 2d surface. the liquid or water(agar or not) is the BROTH.
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