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mushboy
modboy



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Making Liquid Culture 45
#26022486 - 05/30/19 08:27 AM (4 years, 7 months ago) |
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MAKING LIQUID CULTURE
 
LC should ONLY BE MADE from clean agar plates. this is a point of logic not a 'can it be done' statement. for the sake of making the 10 millionth 'why did i fail?' thread, just take my word and DONT MAKE LCs WITH SPORES.
WHAT IS LC? LC would be the growing culture inside a nutrient rich liquid. the liquid is technically called a broth. this is also the case with agar. so if you see the word BROTH, it simply means the liquid you have prepared. AGAR and LC are the same thing(broths). only agar..has agar WHY LC?because mushrooms HOW TO MAKE THE BROTHmeasure out desired amounts of water. i like doing ~250ml water per broth. you can make gallon LCs if you want  i do smaller amounts because they colonize quicker and 250ml of LC can last a long time. also cuts back on how much media you expose while inoculating and so on.. INOCULATION TEKSJOSEX BIOPSY POKE - mandatory reading  WEDGE METHOD(tek TBA) LETS MAKE THE BROTH  weigh out 1gram of LME(light malt extract..powder!) i like doing ~1g per ~250ml water.   add the powder first THEN add COLD water. this prevents clumping. do NOT use hot water to make broths the hot water can cook the grain powder giving you lumps shake it up good.. sometimes i stash the prepared broth in the fridge for awhile(after grain cycles run) but you dont have too. cover with foil, back off the cap and pc 15min @ 15psi.  liquids sterilize easily. 30min+ can caramelize the sugars/wastes time. i remove from the PC the moment psi hits zero and tighten the cap other pointsunused broths have long shelf lifes. ive let them sit for weeks and they are ready for inoculation when you are. i use unmodified lids. i screw the lid on tight after inoculation. the culture is suspended in liquid and they dont need much GE anyway. NUTE TYPES:you can use many nute sources to make broths. as with agar, the type of nute will determine clarity LIGHT MALT EXTRACT BRF unfiltered GRAIN WATER ive really only made three types. light malt, brf and grain water. LC DISCLAIMERsome people say lc is advanced or contamination prone ect.. IME this is not correct. however, LC can and will exploit any cracks in your cultivation armour. grains prepped too wet? cultures not entirely clean? SAB technique not down?? youll grow nothing but green real truthhoney/sugary LCs are so 1995
Edited by mushboy (05/30/19 08:59 AM)
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mushboy
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Re: Making Liquid Culture [Re: micelio] 6
#26022670 - 05/30/19 10:03 AM (4 years, 7 months ago) |
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i just pour them and eye ball it.
250ml of lc is enough for at least 25 jars. 50 if you got skills. i usually get about 30ish but im lazy like that.
i dont shake after inoculation either. just let them chill. i need to know if i contaminate from the liquid or from technique. impossible to tell if you shake shit up right away.
this means you did it correctly
 
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mushboy
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Re: Making Liquid Culture [Re: SFS96] 5
#26022636 - 05/30/19 09:50 AM (4 years, 7 months ago) |
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i like using media bottles for LC because its easy to pour into grain jars.
the mechanics of inoculating the broth and then using the lc to pour into grain jars is the same as pouring petris or doing g2g/a2g. easy peasy
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ShaperDreaming
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Re: Making Liquid Culture [Re: mushboy] 4
#26022640 - 05/30/19 09:53 AM (4 years, 7 months ago) |
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Nice! This ... kinda makes a tek I was going to write up unnecessary! Good work
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Asura
Cyantist


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Re: Making Liquid Culture [Re: Mr. Alien] 4
#26371981 - 12/09/19 10:20 PM (4 years, 1 month ago) |
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I like it when it looks like a horse blew a load in the jar
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mushboy
modboy



Registered: 04/24/05
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ooops.
post it anyway! maybe i forgot something or youll explain it in a way that will connect with more people
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ShaperDreaming
Weirdo



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Re: Making Liquid Culture [Re: micelio] 3
#26046244 - 06/11/19 04:14 PM (4 years, 7 months ago) |
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I just swirl it like it's a wine glass. I'm half tempted to get magnetic stirrers and a stirring plate for no other reason than I think it would look bad ass and super science-y!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: Making Liquid Culture [Re: Mr. Alien] 3
#26368517 - 12/08/19 08:21 AM (4 years, 1 month ago) |
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Quote:
Mr. Alien said: Contaminations can crawl up and enter thru the lid? i doubt? I have media bottle with some agar leftovers. Seems like a good experiment. I thought only gases could do that.
Anyway... so if is TIGHT the air inside the bottle is enough for GE?
I would assume there's more than enough air for GE in the bottles. There's probably enough air for a housefly to live a couple days let alone mycelium for weeks.
Also contaminated air cannot travel through obscure pathways in all circumstances. Pasteur built contraptions to prove germ theory like this
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bodhisatta 
Smurf real estate agent


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Re: Making Liquid Culture [Re: bodhisatta] 3
#26368522 - 12/08/19 08:26 AM (4 years, 1 month ago) |
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 Better picture
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ShaperDreaming
Weirdo



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Re: Making Liquid Culture [Re: mushboy] 2
#26022675 - 05/30/19 10:07 AM (4 years, 7 months ago) |
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Quote:
mushboy said: ooops.
post it anyway! maybe i forgot something or youll explain it in a way that will connect with more people
Haha, no oops, solid work here form a TC. I'll likely still post it, but it's been lowered in priority now
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sandman420
Saint PP



Registered: 06/17/04
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Re: Making Liquid Culture [Re: Camera93] 2
#26022800 - 05/30/19 11:25 AM (4 years, 7 months ago) |
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Wow 0.4% malt, that's pretty low. I might have to try that and cut back on my malt usage haha. Higher percents like 1-2% definitely give more sediments with malt, no problem though. nice tek bud.
Edited by sandman420 (05/30/19 11:30 AM)
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sandman420
Saint PP



Registered: 06/17/04
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Yea josex probably just meant use the whole jar of LC at once, don't take out half and use it and expect to use the other half a week later. I think that is good advice.
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micelio
Song of Silence


Registered: 04/22/14
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I'm half tempted to get a glass of wine..Ha..
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verum subsequentis
seeker of truth



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I'd highly recommend getting stir bars and making our buying a stirrer. It's not needed but makes the LC grow faster, the myc status in tiny tiny little pieces with no clumps and it's truly set and forget.
You can absolutely use half, reload the SAB, and use the other half. You can even use half, let it sit for a couple weeks and use the other half. Just remember with LC, one wrong move and everything after that goes to shit. I highly recommend getting an LC on agar three or four days before use. Even if it's already been tested and used prior. If it's been sitting long, I test it again.
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verum subsequentis
seeker of truth



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Re: Making Liquid Culture [Re: newtomyc] 2
#26046506 - 06/11/19 06:19 PM (4 years, 7 months ago) |
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Use it! You'll be surprised
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sandman420
Saint PP



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Re: Making Liquid Culture [Re: Bph] 2
#26046656 - 06/11/19 07:17 PM (4 years, 7 months ago) |
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Sure you can keep reusing it but there's an increased chance that some bacteria could grow on your ports or in the jar from a dirty operation and have time to grow. If you are using it all at once at least it won't all go bad from a single mistake. Just better chances that's all. LC can be a prickly whore when it goes wrong. I personally size my LC jars to have exactly what I need for one "batch" and it all works out good.
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Dactylium
Don't touch me I'm sterile

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Quote:
verum subsequentis said: @shaper I only use plastic lids and always wrap them. Works like a charm, even for LC that I swirl vigorously (I'm sure it gets up in the threads)
I wrap the threads of my jars several times with plumber's tape to form a nice seal with my plastic lids.
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verum subsequentis
seeker of truth



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Re: Making Liquid Culture [Re: newtomyc] 2
#26054732 - 06/15/19 09:27 PM (4 years, 7 months ago) |
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I'm not mushboy but... The more the better, in my opinion.
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verum subsequentis
seeker of truth



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Re: Making Liquid Culture [Re: newtomyc] 2
#26069230 - 06/23/19 12:08 PM (4 years, 7 months ago) |
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I've never seen a liquid become "fully" colonized. If you let it settle and it's 90% myc it's as fine as it'll get. I believe by that point the culture has consumed ask available nutes. But I use 2% malt.... So...
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Bph
Stranger



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Re: Making Liquid Culture [Re: Asura] 2
#26079829 - 06/28/19 07:57 PM (4 years, 6 months ago) |
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Cool. Think I'll stick to the marbles. It shouldn't be a trigger go but between the alcohol fumes and the syringes it is. Hate nipple rings but love spoon fulls of dope.
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