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InvisiblemushboyMDiscord
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Making Liquid Culture * 45
    #26022486 - 05/30/19 08:27 AM (4 years, 7 months ago)

:stirthepot:MAKING LIQUID CULTURE:stirthepot:



LC should ONLY BE MADE from clean agar plates. this is a point of logic not a 'can it be done' statement.
for the sake of making the 10 millionth 'why did i fail?' thread, just take my word and DONT MAKE LCs WITH SPORES.





WHAT IS LC?

LC would be the growing culture inside a nutrient rich liquid. the liquid is technically called a broth. this is also the case with agar.
so if you see the word BROTH, it simply means the liquid you have prepared.
AGAR and LC are the same thing(broths). only agar..has agar:stoned:

WHY LC?
because mushrooms:shrug:

HOW TO MAKE THE BROTH
measure out desired amounts of water. i like doing ~250ml water per broth. you can make gallon LCs if you want:shrug:
i do smaller amounts because they colonize quicker and 250ml of LC can last a long time. also cuts back on how much media you expose while inoculating and so on..

INOCULATION TEKS
JOSEX BIOPSY POKE - mandatory reading:oldman:
WEDGE METHOD(tek TBA)

LETS MAKE THE BROTH

weigh out 1gram of LME(light malt extract..powder!) i like doing ~1g per ~250ml water.

add the powder first THEN add COLD water. this prevents clumping.

do NOT use hot water to make broths:nono: the hot water can cook the grain powder giving you lumps:facepalm:

shake it up good..

sometimes i stash the prepared broth in the fridge for awhile(after grain cycles run) but you dont have too.

cover with foil, back off the cap and pc 15min @ 15psi.

liquids sterilize easily. 30min+ can caramelize the sugars/wastes time.
i remove from the PC the moment psi hits zero and tighten the cap:thumbup:

other points
unused broths have long shelf lifes. ive let them sit for weeks and they are ready for inoculation when you are.
i use unmodified lids. i screw the lid on tight after inoculation. the culture is suspended in liquid and they dont need much GE anyway.

NUTE TYPES:
you can use many nute sources to make broths. as with agar, the type of nute will determine clarity:thumbup:

LIGHT MALT EXTRACT


BRF unfiltered


GRAIN WATER


ive really only made three types. light malt, brf and grain water.

LC DISCLAIMER
some people say lc is advanced or contamination prone ect.. IME this is not correct. however, LC can and will exploit any cracks in your cultivation armour.
grains prepped too wet? cultures not entirely clean? SAB technique not down?? youll grow nothing but green:sad:


real truth
honey/sugary LCs are so 1995
:begone:


Edited by mushboy (05/30/19 08:59 AM)


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: micelio] * 6
    #26022670 - 05/30/19 10:03 AM (4 years, 7 months ago)

i just pour them and eye ball it.

250ml of lc is enough for at least 25 jars. 50 if you got skills. i usually get about 30ish but im lazy like that.

i dont shake after inoculation either. just let them chill. i need to know if i contaminate from the liquid or from technique. impossible to tell if you shake shit up right away.

this means you did it correctly




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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: SFS96] * 5
    #26022636 - 05/30/19 09:50 AM (4 years, 7 months ago)

:rockon:

i like using media bottles for LC because its easy to pour into grain jars.

the mechanics of inoculating the broth and then using the lc to pour into grain jars is the same as pouring petris or doing g2g/a2g. easy peasy


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: mushboy] * 4
    #26022640 - 05/30/19 09:53 AM (4 years, 7 months ago)

Nice! This ... kinda makes a tek I was going to write up unnecessary! Good work :smile:


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OfflineAsuraS
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Re: Making Liquid Culture [Re: Mr. Alien] * 4
    #26371981 - 12/09/19 10:20 PM (4 years, 1 month ago)

I like it when it looks like a horse blew a load in the jar



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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: ShaperDreaming] * 3
    #26022643 - 05/30/19 09:54 AM (4 years, 7 months ago)

ooops.

post it anyway! maybe i forgot something or youll explain it in a way that will connect with more people:shrug:


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: micelio] * 3
    #26046244 - 06/11/19 04:14 PM (4 years, 7 months ago)

I just swirl it like it's a wine glass. I'm half tempted to get magnetic stirrers and a stirring plate for no other reason than I think it would look bad ass and super science-y!

:superscience:


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InvisiblebodhisattaMDiscordReddit
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Re: Making Liquid Culture [Re: Mr. Alien] * 3
    #26368517 - 12/08/19 08:21 AM (4 years, 1 month ago)

Quote:

Mr. Alien said:
Contaminations can crawl up and enter thru the lid? i doubt? I have media bottle with some agar leftovers. Seems like a good experiment. I thought only gases could do that.

Anyway... so if is TIGHT the air inside the bottle is enough for GE?



I would assume there's more than enough air for GE in the bottles. There's probably enough air for a housefly to live a couple days let alone mycelium for weeks.


Also contaminated air cannot travel through obscure pathways in all circumstances. Pasteur built contraptions to prove germ theory like this


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InvisiblebodhisattaMDiscordReddit
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Re: Making Liquid Culture [Re: bodhisatta] * 3
    #26368522 - 12/08/19 08:26 AM (4 years, 1 month ago)


Better picture


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: mushboy] * 2
    #26022675 - 05/30/19 10:07 AM (4 years, 7 months ago)

Quote:

mushboy said:
ooops.

post it anyway! maybe i forgot something or youll explain it in a way that will connect with more people:shrug:



Haha, no oops, solid work here form a TC. I'll likely still post it, but it's been lowered in priority now :wink:


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Invisiblesandman420
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Re: Making Liquid Culture [Re: Camera93] * 2
    #26022800 - 05/30/19 11:25 AM (4 years, 7 months ago)

Wow 0.4% malt, that's pretty low. I might have to try that and cut back on my malt usage haha. Higher percents like 1-2% definitely give more sediments with malt, no problem though. nice tek bud.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (05/30/19 11:30 AM)


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Invisiblesandman420
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Re: Making Liquid Culture [Re: MLPismyOPSEC] * 2
    #26046182 - 06/11/19 03:39 PM (4 years, 7 months ago)

Yea josex probably just meant use the whole jar of LC at once, don't take out half and use it and expect to use the other half a week later. I think that is good advice.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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Invisiblemicelio
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Re: Making Liquid Culture [Re: ShaperDreaming] * 2
    #26046296 - 06/11/19 04:35 PM (4 years, 7 months ago)

I'm half tempted to get a glass of wine..Ha..


--------------------
     


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: ShaperDreaming] * 2
    #26046344 - 06/11/19 05:01 PM (4 years, 7 months ago)

I'd highly recommend getting stir bars and making our buying a stirrer. It's not needed but makes the LC grow faster, the myc status in tiny tiny little pieces with no clumps and it's truly set and forget.

You can absolutely use half, reload the SAB, and use the other half. You can even use half, let it sit for a couple weeks and use the other half. Just remember with LC, one wrong move and everything after that goes to shit. I highly recommend getting an LC on agar three or four days before use. Even if it's already been tested and used prior. If it's been sitting long, I test it again.


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: newtomyc] * 2
    #26046506 - 06/11/19 06:19 PM (4 years, 7 months ago)

Use it! You'll be surprised


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Invisiblesandman420
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Re: Making Liquid Culture [Re: Bph] * 2
    #26046656 - 06/11/19 07:17 PM (4 years, 7 months ago)

Sure you can keep reusing it but there's an increased chance that some bacteria could grow on your ports or in the jar from a dirty operation and have time to grow. If you are using it all at once at least it won't all go bad from a single mistake. Just better chances that's all. LC can be a prickly whore when it goes wrong. I personally size my LC jars to have exactly what I need for one "batch" and it all works out good.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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OfflineDactylium
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Re: Making Liquid Culture [Re: verum subsequentis] * 2
    #26050415 - 06/13/19 02:58 PM (4 years, 7 months ago)

Quote:

verum subsequentis said:
@shaper I only use plastic lids and always wrap them. Works like a charm, even for LC that I swirl vigorously (I'm sure it gets up in the threads)




I wrap the threads of my jars several times with plumber's tape to form a nice seal with my plastic lids.


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: newtomyc] * 2
    #26054732 - 06/15/19 09:27 PM (4 years, 7 months ago)

I'm not mushboy but... The more the better, in my opinion.


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: newtomyc] * 2
    #26069230 - 06/23/19 12:08 PM (4 years, 7 months ago)

I've never seen a liquid become "fully" colonized. If you let it settle and it's 90% myc it's as fine as it'll get. I believe by that point the culture has consumed ask available nutes. But I use 2% malt.... So...


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InvisibleBph
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Re: Making Liquid Culture [Re: Asura] * 2
    #26079829 - 06/28/19 07:57 PM (4 years, 6 months ago)

Cool. Think I'll stick to the marbles. It shouldn't be a trigger go but between the alcohol fumes and the syringes it is. Hate nipple rings but love spoon fulls of dope.


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