Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < First | | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Re: Making Liquid Culture [Re: Failboat]
    #26284195 -

:whathesaid:

Myc will gobble up sediment clear lc is mostly aesthetic :thumbup:

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: mushboy]
    #26284199 -

mushboy said:
:whathesaid:

Myc will gobble up sediment clear lc is mostly aesthetic :thumbup:



Ok great

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: TedsDead]
    #26284345 -

TedsDead said:
@mr alien:  that thing looks like a breast milk pump or something. :facepalm3: 



Yeah I figured. :rolleyes:

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Mr. Alien]
    #26284817 -

Mr. Alien said:
Edit 2: https://www.amazon.com/Microlit-SCIT-60-Bottletop-Dispenser/dp/B07C1VWV1S/ref=sr_1_5?keywords=Dispensette%2Bpp&qid=1572229750&sr=8-5&th=1

What do you guys think about that autoclavable dispenser than can be adapted to media bottles? Seems like the holy grail for pouring :eek:.




I made this from a ketchup bottle,

info here
https://www.shroomery.org/forums/showflat.php/Number/23486437

you can squeeze out pretty accurate repeatable amounts once you get the hang of it. Think my biggest ketchup bottle is about 900ml

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: blackout]
    #26284824 -

Interesting!


How does the syringe stay on the nipple top? Is it just a snug fit so it stays put or did you put something there?


--------------------

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: blackout]
    #26284832 -


Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: blackout]
    #26284859 -

blackout said:
Mr. Alien said:
Edit 2: https://www.amazon.com/Microlit-SCIT-60-Bottletop-Dispenser/dp/B07C1VWV1S/ref=sr_1_5?keywords=Dispensette%2Bpp&qid=1572229750&sr=8-5&th=1

What do you guys think about that autoclavable dispenser than can be adapted to media bottles? Seems like the holy grail for pouring :eek:.




I made this from a ketchup bottle,

info here
https://www.shroomery.org/forums/showflat.php/Number/23486437

you can squeeze out pretty accurate repeatable amounts once you get the hang of it. Think my biggest ketchup bottle is about 900ml



But then it doesn't suck air inside when you squeeze the bottle? I found the amazon product interesting because it suppose to not suck air inside

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Mr. Alien]
    #26285083 -

Mr. Alien said:
But then it doesn't suck air inside when you squeeze the bottle?



yes, I talk about that in the link. Another one I used was a thin silicone tube attached to the bottom of a bottle with a syringe needle shoved into it and then plugged the needle by sticking it into a rubber bung. Then I have a filtered jar and just take the bung off and the liquid can flow through the needle, I have little clamps for the tube, you can just pinch it while doing a few jars. Now the air is sucked in through the filter on the jar. A needle is not even needed, it can be clumped easier than a syringe as there is only the pressure of the head of liquid rather than a syringe plunger, however the tubing can be massaged or "milked" to create pressure. I like the needle for fine control but if filling lots of jars a narrow tube is good enough. I have plugged narrow silicone tube with the head of a rounded stainless screw, this can be difficult to replug in a sterile manner so better to be a single session of filling.

Your bottle could be outside of the SAB and up high to create more pressure and higher flow rates. People doing big grows could easily make huge amounts of LC. I always wanted to try doing it in a large bucket. You can sterilize concentrated LC in a PC and then dilute it in water in a bucket with a heating element in it and a filtered lid. Now a simple boil is all that should be needed to resterilize, with your element ideally connected to a variable power supply.

Quote:
Mr. Alien said: I found the amazon product interesting because it suppose to not suck air inside



are you sure it doesn't? a vacuum would be forming in the bottle if sucking liquid out. I guess if there is an air intake it could be filtered but I didn't see anything on the amazon page that specifically said no air was going in.

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: blackout]
    #26298278 -

Started my first (and second, and third, and fourth... :lol:) LCs. The K stands for Karo as i wanted to try Asura's 2.5g LME/10g dextrose/250mL H2O recipe as well, for schnitzengiggle. Used a small agar wedge, and so far they are all looking like i have some action! Fingers crossed, i'm pretty excited.


Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: MLPismyOPSEC]
    #26298293 -

Plantasia mystery huh? Those were fun and weird at the same time. I had a first flush that was only one gigantic mushroom and then a bunch of short oval capped ones came later.


--------------------


Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:



Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: blackout]
    #26302805 -

blackout said:
Quote:
Mr. Alien said: I found the amazon product interesting because it suppose to not suck air inside



are you sure it doesn't? a vacuum would be forming in the bottle if sucking liquid out. I guess if there is an air intake it could be filtered but I didn't see anything on the amazon page that specifically said no air was going in.



You were right it does suck air inside the bottle, they recommended installing a membrane filter to avoid contamination.

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: blackout]
    #26302856 -

blackout said:
Mr. Alien said:
But then it doesn't suck air inside when you squeeze the bottle?



yes, I talk about that in the link. Another one I used was a thin silicone tube attached to the bottom of a bottle with a syringe needle shoved into it and then plugged the needle by sticking it into a rubber bung. Then I have a filtered jar and just take the bung off and the liquid can flow through the needle, I have little clamps for the tube, you can just pinch it while doing a few jars. Now the air is sucked in through the filter on the jar. A needle is not even needed, it can be clumped easier than a syringe as there is only the pressure of the head of liquid rather than a syringe plunger, however the tubing can be massaged or "milked" to create pressure. I like the needle for fine control but if filling lots of jars a narrow tube is good enough. I have plugged narrow silicone tube with the head of a rounded stainless screw, this can be difficult to replug in a sterile manner so better to be a single session of filling.

Your bottle could be outside of the SAB and up high to create more pressure and higher flow rates. People doing big grows could easily make huge amounts of LC. I always wanted to try doing it in a large bucket. You can sterilize concentrated LC in a PC and then dilute it in water in a bucket with a heating element in it and a filtered lid. Now a simple boil is all that should be needed to resterilize, with your element ideally connected to a variable power supply.

Quote:
Mr. Alien said: I found the amazon product interesting because it suppose to not suck air inside



are you sure it doesn't? a vacuum would be forming in the bottle if sucking liquid out. I guess if there is an air intake it could be filtered but I didn't see anything on the amazon page that specifically said no air was going in.



That one looks to be for chemical dispensing, so unclean air isn't a problem. There are some that have a filter and others that take external filtered air input. Or use it in a clean room.

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Mr. Alien]
    #26302862 -

First LC's made yesterday per this TEK , one with grain water, the other grain water with some coffee ground oats flour mixed in just to try!



That Josex Biopsy got me nervous ima watch a jar of liquid sit there and do nothing for weeks lol... but trust in the TEKS keeps noobs in check I guess!

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Stampy]
    #26303208 -

That right one is so dark will be difficult to know if anything is growing I would think. I honestly dk cause I've never used something that dark. The left handed one is better (straight grain water one I think?) but it still seems kinda darkish.

I like LC really really light but it may just be my preference. I've been using mushboy's recipe  1gram LME to 500mL of water

Maybe turbid is a better word than dark, visibility is poor, it is kind of actually "light" in colour


--------------------

Edited by sh4d0ws (11/06/19 11:02 PM)

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: sh4d0ws]
    #26303891 -

Hey thanks! So to get a lighter lc I just wouldn't boil the grain water as long right?

Yea the one on the right turned out way too murky but now it has settled a lot so it is a bit better, but I don't think I will be doing that again.

And MEA is malt extract correct? Would you source that from like a brewery or where?

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Stampy]
    #26303903 -

My beer lc is coming along nicely. 50/50 Miller/water. Strain x7x cubes.


--------------------


Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:



Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Stampy]
    #26303942 -

Stampy said:
Hey thanks! So to get a lighter lc I just wouldn't boil the grain water as long right?

Yea the one on the right turned out way too murky but now it has settled a lot so it is a bit better, but I don't think I will be doing that again.

And MEA is malt extract correct? Would you source that from like a brewery or where?




Hey man
I think if you dilute the grain water some it will help clear it up though I'm not sure how much dilution is too much since I only used grain water LC a couple times but I do remember diluting it and even filtering some sediment out though on later batches I just diluted. The diluted stuff still worked well but I can't remember if it was diluted 50% or more. I think it was 50%.

Once I found LME I started using solely LME for my LC. I misspoke in the earlier post, I meant to say LME.

Light malt extract.

MEA is the abbreviation for malt extract agar which is just agar made using LME and agar agar.

You can find that from a brewing supply store, but I have been buying mine on Amazon :stoned:

:cheers:


--------------------

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: sh4d0ws]
    #26303944 -

Perfect man thanks!!

Next batch I definitely will give it a go!

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Stampy]
    #26307326 -

With a stirplate, do you leave it on a low setting all the time or high speed blend once a day? I don't have one yet, but i want to be prepared if/when i get one.

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: MLPismyOPSEC]
    #26307350 -

a timer is best. I do 3-4 20 second stirs a day(cycle timer)  if you leave it on all the time you can kill the myc.


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

Extras: Filter Print Post Top
Jump to top Pages: < First | | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | | Last >

Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* 1st Liquid Culture, arrrgh! Seril 15,569 10 02/28/06 08:35 PM
by Limelight_Liver
* Starting a simple liquid culture method Garret_Hill 9,911 3 03/11/02 09:49 AM
by YourHighness
* Easy Liquid Culture Container Tek SixTango 21,739 7 01/17/12 04:59 PM
by dubyastep
* Liquid Cultures to Bulk Substrate Prodijal_Son 19,170 7 05/05/20 03:40 AM
by poisoned
* liquid culture failure gray1 6,470 3 07/19/01 09:37 PM
by Azure
* liquid culture using h2o2 baja 9,932 14 03/09/17 10:00 PM
by 3B3B4E
* Liquid culture inoculation of straw... possible? The FUnGal FiEnD 7,977 9 05/26/13 04:57 PM
by cronicr
* Liquid culture for alkaloids?? Dr. Slavic 969 3 01/26/03 06:29 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
94,404 topic views. 17 members, 842 guests and 58 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.038 seconds spending 0.01 seconds on 15 queries.