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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 2 days, 11 hours
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i also have repurposed 6 snapple bottles. 3 different varieties done 2 ways one just lme and the other lme and rye flour. The rye bottles are a bit cloudy and had sediment, but the mycelium colonized the sediment, or it's trash, but I think they're all good. Got BHT, MNAPE, amd Tamps or Hoogs, I forget right meow. They were inoculated on the 4th. Rather thick myc in all, but still color in the broth so I'm going to keep waiting until next week. I'm thinking LC>Millet Masters>winter wheat
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 1 day
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Re: Making Liquid Culture [Re: Failboat]
#26204212 - 09/23/19 08:42 AM (4 years, 4 months ago) |
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I also use media bottles without filter. I do use a filter on my mason jars though, mostly because I have so damn many modded one already it's easier to find them often than non-modded.
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
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Turns out I have MNAPE, Tamps, and Hoog LC. The Tamps are trying to make stones hah
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
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Re: Making Liquid Culture [Re: Failboat]
#26204290 - 09/23/19 09:46 AM (4 years, 4 months ago) |
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Quote:
Quirkmeister92 said: The Tamps are trying to make stones hah
In the LC?! Amazing.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,263
Loc: where?
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Pix
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 2 days, 11 hours
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Re: Making Liquid Culture [Re: mushboy]
#26204629 - 09/23/19 12:29 PM (4 years, 4 months ago) |
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 So if I recognize what all I have noticed they started on the sides of the glass aboulve the liquid level cause when I shake em like bubbly bits of myc get stuck on the sides. When i gave it a swirl most of the growth on the glass got washed back in and now seems to be developing on the surface.
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ChardRich
chardzard



Registered: 04/16/19
Posts: 1,167
Loc: Upper Left USA
Last seen: 1 year, 5 days
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Re: Making Liquid Culture [Re: mushboy]
#26213547 - 09/27/19 01:15 PM (4 years, 3 months ago) |
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Well, I am about a week in. These pics are during a good swirl. I know I used a lot of agar, but I wanted to get something cooking while I worked on dialing in some cultures.
How does it look?
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 1 day
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Re: Making Liquid Culture [Re: ChardRich] 1
#26213644 - 09/27/19 01:50 PM (4 years, 3 months ago) |
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Looks like marimo moss balls Doing good! Next time you may want something in there to agitate the mycelium, like a hunk of broken glass. I found that after I got the stir plate that the best part was how it breaks up the mycelium so that it doesn't clog up syringes, or blob up in a pour.
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vinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
Posts: 492
Last seen: 8 months, 2 days
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I want to use this to make a liquid culture. How much should I use per liter to achieve 4% nutrients? Also, I have dextrose Mannitol lactose and sucrose. It's for panaeolus. Which do you guys think would be best?
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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4% by weight. So 4g for 100ml solution, 8g for 200ml, 20g for 500ml but honestly 4% is pretty high.
I do like .5% if I even measure. Just a pinch is all you really need
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vinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
Posts: 492
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Damn I feel like an idiot now LOL
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 23 days
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Quote:
vinnie boombotz said:

I want to use this to make a liquid culture.
That is some sort of protein powder, I would not use it. Even if it works it will be very murky and hard to see what is going on.
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ChardRich
chardzard



Registered: 04/16/19
Posts: 1,167
Loc: Upper Left USA
Last seen: 1 year, 5 days
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Re: Making Liquid Culture [Re: blackout]
#26236323 - 10/07/19 07:18 PM (4 years, 3 months ago) |
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So what happens when/why is it bad to pour too much LC?
I try and pour the smallest amount, but sometimes i get too much
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
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Re: Making Liquid Culture [Re: ChardRich]
#26236402 - 10/07/19 07:53 PM (4 years, 3 months ago) |
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depends on how you prepped the grain.
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ChardRich
chardzard



Registered: 04/16/19
Posts: 1,167
Loc: Upper Left USA
Last seen: 1 year, 5 days
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Bone dry baby, bone dry
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Making Liquid Culture [Re: ChardRich]
#26236415 - 10/07/19 07:59 PM (4 years, 3 months ago) |
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I've left LC pooled up in the bottom of wbs bags that were prepped super dry and always had it colonize fine. I once put 100+... i actually forget... i think 160 cc's in one bag. It did fine. But it was prepped very dry
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AyePlus
Stony Danza


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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What do you guys think about these LC test plates? Iβm leaning towards using the AA+ but the MMc might be cleaner? Looking for more opinions..
Edit: Sorry for the shitty photoβs, I tried..
-------------------- Learn about breeding
  C10βs agar guide Good surface conditions = Good pinsets Read more, post less. π
π° πΌ π΄ π
π΄ π° πΌ π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Making Liquid Culture [Re: AyePlus]
#26237752 - 10/08/19 12:08 PM (4 years, 3 months ago) |
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It's much easier to tell when streaked but i think you're fine. I THINK!
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k5hd2y
Depersonalized & Complete


Registered: 04/13/17
Posts: 675
Last seen: 4 years, 2 months
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100ml-125ml works well for me in each bag of 4.5-5qts grain.
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k5hd2y
Depersonalized & Complete


Registered: 04/13/17
Posts: 675
Last seen: 4 years, 2 months
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Re: Making Liquid Culture [Re: k5hd2y]
#26237774 - 10/08/19 12:16 PM (4 years, 3 months ago) |
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I don't test because I'm scared of the extra vector. lol When i open a jar of LC, it's getting poured.
All or Nothing
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