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OfflineFailboat
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Re: Making Liquid Culture [Re: natedawgnow]
    #26199173 - 09/20/19 11:59 AM (4 years, 4 months ago)

:whathesaid: i also have repurposed 6 snapple bottles. 3 different varieties done 2 ways one just lme and the other lme and rye flour. The rye bottles are a bit cloudy and had sediment, but the mycelium colonized the sediment, or it's trash, but I think they're all good. Got BHT, MNAPE, amd Tamps or Hoogs, I forget right meow. They were inoculated on the 4th. Rather thick myc in all, but still color in the broth so I'm going to keep waiting until next week. I'm thinking LC>Millet Masters>winter wheat


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: Failboat]
    #26204212 - 09/23/19 08:42 AM (4 years, 4 months ago)

I also use media bottles without filter. I do use a filter on my mason jars though, mostly because I have so damn many modded one already it's easier to find them often than non-modded.


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OfflineFailboat
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Re: Making Liquid Culture [Re: ShaperDreaming]
    #26204215 - 09/23/19 08:45 AM (4 years, 4 months ago)

Turns out I have MNAPE, Tamps, and Hoog LC. The Tamps are trying to make stones hah


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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: Failboat]
    #26204290 - 09/23/19 09:46 AM (4 years, 4 months ago)

Quote:

Quirkmeister92 said:
The Tamps are trying to make stones hah



In the LC?! Amazing.


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: ShaperDreaming]
    #26204615 - 09/23/19 12:21 PM (4 years, 4 months ago)

Pix:begger:


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OfflineFailboat
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Re: Making Liquid Culture [Re: mushboy]
    #26204629 - 09/23/19 12:29 PM (4 years, 4 months ago)


So if I recognize what all I have noticed they started on the sides of the glass aboulve the liquid level cause when I shake em like bubbly bits of myc get stuck on the sides. When i gave it a swirl most of the growth on the glass got washed back in and now seems to be developing on the surface.


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OfflineChardRich
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Registered: 04/16/19
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Re: Making Liquid Culture [Re: mushboy]
    #26213547 - 09/27/19 01:15 PM (4 years, 3 months ago)

Well, I am about a week in. These pics are during a good swirl. I know I used a lot of agar, but I wanted to get something cooking while I worked on dialing in some cultures.

How does it look?



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OfflineShaperDreaming
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Re: Making Liquid Culture [Re: ChardRich] * 1
    #26213644 - 09/27/19 01:50 PM (4 years, 3 months ago)

Looks like marimo moss balls :lol: Doing good! :thumbup: Next time you may want something in there to agitate the mycelium, like a hunk of broken glass. I found that after I got the stir plate that the best part was how it breaks up the mycelium so that it doesn't clog up syringes, or blob up in a pour.


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Offlinevinnie boombotz
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Re: Making Liquid Culture [Re: ShaperDreaming]
    #26231899 - 10/05/19 03:20 PM (4 years, 3 months ago)



I want to use this to make a liquid culture. How much should I use per liter to achieve 4% nutrients? Also, I have dextrose Mannitol lactose and sucrose. It's for panaeolus. Which do you guys think would be best?


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Invisiblenatedawgnow
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Re: Making Liquid Culture [Re: vinnie boombotz] * 2
    #26232497 - 10/05/19 08:21 PM (4 years, 3 months ago)

4% by weight. So 4g for 100ml solution, 8g for 200ml, 20g for 500ml but honestly 4% is pretty high.

I do like .5% if I even measure. Just a pinch is all you really need


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Offlinevinnie boombotz
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Re: Making Liquid Culture [Re: natedawgnow]
    #26232607 - 10/05/19 09:35 PM (4 years, 3 months ago)

Damn I feel like an idiot now LOL


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Offlineblackout
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Re: Making Liquid Culture [Re: vinnie boombotz]
    #26233333 - 10/06/19 10:58 AM (4 years, 3 months ago)

Quote:

vinnie boombotz said:


I want to use this to make a liquid culture.



That is some sort of protein powder, I would not use it. Even if it works it will be very murky and hard to see what is going on.


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OfflineChardRich
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Re: Making Liquid Culture [Re: blackout]
    #26236323 - 10/07/19 07:18 PM (4 years, 3 months ago)

So what happens when/why is it bad to pour too much LC?

I try and pour the smallest amount, but sometimes i get too much :shrug:


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: ChardRich]
    #26236402 - 10/07/19 07:53 PM (4 years, 3 months ago)

depends on how you prepped the grain.


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OfflineChardRich
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26236406 - 10/07/19 07:54 PM (4 years, 3 months ago)

Bone dry baby, bone dry

:puddingpop:


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: ChardRich]
    #26236415 - 10/07/19 07:59 PM (4 years, 3 months ago)

I've left LC pooled up in the bottom of wbs bags that were prepped super dry and always had it colonize fine. I once put 100+... i actually forget... i think 160 cc's in one bag. It did fine. But it was prepped very dry


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InvisibleAyePlusS
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26237731 - 10/08/19 11:58 AM (4 years, 3 months ago)








What do you guys think about these LC test plates? I’m leaning towards using the AA+ but the MMc might be cleaner? Looking for more opinions..

Edit: Sorry for the shitty photo’s, I tried..


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: AyePlus]
    #26237752 - 10/08/19 12:08 PM (4 years, 3 months ago)

It's much easier to tell when streaked but i think you're fine. I THINK!


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Offlinek5hd2y
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Re: Making Liquid Culture [Re: verum subsequentis] * 1
    #26237763 - 10/08/19 12:12 PM (4 years, 3 months ago)

100ml-125ml works well for me in each bag of 4.5-5qts grain.


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Offlinek5hd2y
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Re: Making Liquid Culture [Re: k5hd2y]
    #26237774 - 10/08/19 12:16 PM (4 years, 3 months ago)

I don't test because I'm scared of the extra vector.  lol
When i open a jar of LC, it's getting poured.

All or Nothing


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