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 The FUnGal FiEnD
Mister, can youplease help mypony?
Registered: 01/30/03
Posts: 133
Last seen: 14 years, 10 months
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 Liquid culture inoculation of straw... possible? 
#1328195 - 02/23/03 08:30 AM (15 years, 3 months ago) |
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a buddy just got ripped on a $500 kit (probably from STP). instead of a substrate like rye, millet, or anything like that, it came with LCIP (liquid culture inoculum powder) which is probably just dextrose or some shit. It says to grow mycelia till liquid is 50% colonized, then pour right onto the straw. Im worried that the huge amount of liquid used will invite contams before the mycelia can take hold, and due to the large size of the kit, that would be really bad. Is it feasible, or should i just use the colonized LCIP to inoculate grain jars? 
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"Don't believe the florist when he tells you that the roses are free." -- Ween
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The FUnGal FiEnD
Mister, can youplease help mypony?
Registered: 01/30/03
Posts: 133
Last seen: 14 years, 10 months
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 Re: Liquid culture inoculation of straw... possible? [Re: The FUnGal FiEnD]
#1330975 - 02/24/03 09:08 AM (15 years, 3 months ago) |
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anyone?
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"Don't believe the florist when he tells you that the roses are free." -- Ween
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cryanbeginner
Stranger
Registered: 05/26/13
Posts: 16
Last seen: 5 years, 14 days
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Re: Liquid culture inoculation of straw... possible? [Re: The FUnGal FiEnD]
#18324114 - 05/26/13 06:39 PM (5 years, 24 days ago) |
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im trying to figure the same thing I have a colonized lc of water and honey ND WANT TO KNOW IF I CAN PUT THE LC STRAIGHT INTO SHEEP COW DUNG STRAW MIX
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Smkymtnagar
Intrepid Student
Registered: 01/15/13
Posts: 126
Loc: USA 
Last seen: 1 year, 5 months
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Re: Liquid culture inoculation of straw... possible? [Re: cryanbeginner]
#18324125 - 05/26/13 06:41 PM (5 years, 24 days ago) |
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Necromancy
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Jman420
MagicJ
Registered: 12/23/12
Posts: 866
Loc: On a Cloud
Last seen: 2 years, 1 month
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Re: Liquid culture inoculation of straw... possible? [Re: cryanbeginner]
#18324138 - 05/26/13 06:45 PM (5 years, 24 days ago) |
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I'd use it to inoculate grain jars...then use those jars (after they have colonized 100%) with your straw sub. I would think at 50% colonization would be an inviting home for open-air contaminates to find their way inside. Not to mention its a LC..ever more the reason to take extra sterile precautions.
This is just my thoughts/what I'd do though.
Gl 
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If you want to become an expert, you'll need to do a lot more research.
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cryanbeginner
Stranger
Registered: 05/26/13
Posts: 16
Last seen: 5 years, 14 days
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Re: Liquid culture inoculation of straw... possible? [Re: The FUnGal FiEnD]
#18324149 - 05/26/13 06:49 PM (5 years, 24 days ago) |
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I got my lc from et it came with a syringe of peroxide to put in it after its fully colonized to not get contaminated
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cronicr
Registered: 08/07/11
Posts: 56,249
Loc: Van Isle 
Last seen: 18 hours, 18 minutes
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Re: Liquid culture inoculation of straw... possible? [Re: cryanbeginner]
#18324158 - 05/26/13 06:50 PM (5 years, 24 days ago) |
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Quote:
cryanbeginner said:
im trying to figure the same thing I have a colonized lc of water and honey ND WANT TO KNOW IF I CAN PUT THE LC STRAIGHT INTO SHEEP COW DUNG STRAW MIX
WE GAVE YOU AN ANSWER, STOP YELLING AT US! YOU CAN TRY, IT MIGHT WORK! BUT ITS A GAMBLE THAT MOST WON'T TAKE! HAVE A GOOD DAY!
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It doesn't matter what i think of you...all that matters is clean spawn
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cryanbeginner
Stranger
Registered: 05/26/13
Posts: 16
Last seen: 5 years, 14 days
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Re: Liquid culture inoculation of straw... possible? [Re: cronicr]
#18324166 - 05/26/13 06:53 PM (5 years, 24 days ago) |
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accidently hit caps thanx
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,140
Loc: UK 
Last seen: 15 hours, 12 minutes
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Re: Liquid culture inoculation of straw... possible? [Re: cryanbeginner]
#18324184 - 05/26/13 06:56 PM (5 years, 24 days ago) |
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Quote:
cryanbeginner said:
I got my lc from et it came with a syringe of peroxide to put in it after its fully colonized to not get contaminated
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cronicr
Registered: 08/07/11
Posts: 56,249
Loc: Van Isle
Last seen: 18 hours, 18 minutes
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Re: Liquid culture inoculation of straw... possible? [Re: cryanbeginner]
#18324187 - 05/26/13 06:57 PM (5 years, 24 days ago) |
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Quote:
cryanbeginner said:
accidently hit caps thanx
 all good
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It doesn't matter what i think of you...all that matters is clean spawn
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