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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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OfflineProdijal_Son
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Registered: 07/20/02
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Liquid Cultures to Bulk Substrate
    #1192686 - 01/05/03 01:11 PM (20 years, 8 months ago)

Been reading up on liquid cultures.

Read that there a bit more susceptible to contam's than other methods. Anyone have any experience with that?

When one uses liquid cultures ... do I still have to use jars ... then go to a bulk substrate ... or can i just innoc a bed of straw with the liq sub?

Does anyone have any sweet personal teks on liquid substrates? I think that I'm going with the potato aka. dextrose method.

I have no idea what this strain isolation is though.

Thanks cats. Late.



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InvisibleSubGen1us

Registered: 11/26/02
Posts: 3,427
Re: Liquid Cultures to Bulk Substrate [Re: Prodijal_Son]
    #1192897 - 01/05/03 02:28 PM (20 years, 8 months ago)

Liquid medium is used to speed up the process of the grain or brf jars. since ur injecting already germinated spores it speed the time up dramatically, if u use em with grains, its even faster cause u can shake. if u inject directly to bulk u will most likely get contams because u have no strong mycelium to fight it.
Liquid medium=>jars=> bulk(poo/straw)


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OfflineISH
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Re: Liquid Cultures to Bulk Substrate [Re: SubGen1us]
    #1229750 - 01/18/03 05:51 PM (20 years, 8 months ago)

Dito. Also Honey can resist contams easily.



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I think of going to the grave without having a psychedelic experience, like going to the grave without having sex. It means that you never figured out what it was all about. The mystery is in the body, and the way the body works itself into nature.
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Anonymous

Post deleted by Administrator [Re: Prodijal_Son]
    #1229952 - 01/18/03 08:01 PM (20 years, 8 months ago)



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OfflineISH
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Re: Liquid Cultures to Bulk Substrate [Re: ]
    #1230075 - 01/18/03 10:00 PM (20 years, 8 months ago)

Since the clone was part of a "fruiting body, does that mean that if final mycelium is cased it will produce more pins easier than just a multispore innoculation would?  That is to say that the mycelium in the whole substarte would have th eability to fruit?


Sound like the way to go.

Laterz guys.
ISH :laugh: 


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I think of going to the grave without having a psychedelic experience, like going to the grave without having sex. It means that you never figured out what it was all about. The mystery is in the body, and the way the body works itself into nature.
- Terence McKenna, Archaic Revival


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OfflineSeussA
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Re: Liquid Cultures to Bulk Substrate [Re: ISH]
    #1230152 - 01/18/03 11:35 PM (20 years, 8 months ago)

If the clone was made from a fruit body, then yes, the mycelium generated from that clone will be fruit bearing as well. The really nice thing about isolates is that everything grows at the same rate. No more picking a few today and a few tomorrow and a few the next day... and all the fruit have the same strength as well. The down side to isolates is that if you pick a bad mother to clone, all your babies will be just as bad.


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OfflineAz88
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Registered: 05/04/20
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Re: Liquid Cultures to Bulk Substrate [Re: ISH]
    #26648863 - 05/04/20 06:34 PM (3 years, 4 months ago)

Quote:

ISH said:
Dito.  Also Honey can resist contams easily.

 


Do you use honey in stead of dextrose or dry malt extract?


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Offlinepoisoned
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Re: Liquid Cultures to Bulk Substrate [Re: Az88]
    #26649669 - 05/05/20 03:40 AM (3 years, 4 months ago)

Honey resists contams until it's diluted.


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