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 SixTango
Mycota
Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
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 Easy Liquid Culture Container Tek 
#1227856 - 01/17/03 09:31 PM (22 years, 12 days ago) |
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Six Tango's Baby Bottle Liquid Culture Tek.
Glass & polycarb baby bottles (BB), some polycarb screw on lids for BB's, as well as most BB nipples with withstand - sterilization.
The nipples have a tiny hole in the top (some have more than one), in which you can insert polyfill (often requires slight enlargement) to allow some gas exchange. The nipples will also withstand piercing from a syringe & reseal - well - when the needle is withdrawn.
Simply fill BB 5/8 to 3/4 full of your preferred liquid nutrient (generally dry light malt / dextrose / honey / nutritional yeast, alone or in combo's), include 2 or 3 glass marbles in the BB & screw on lid w/nipple prepped w/polyfil.
PC for 15 to 20 minutes @ 15 lbs, Remove BB & cover lid with alc swabbed tin foil (if it does not come with a protective cap) & allow BB to cool to room temp..
When cooled, swab nipple with alc & inject a few CC's of spore solution right through the nipple material, into the BB. Store in a warm (78 to 84 F) dark clean place to incubate. Spores will generally germinate in a few days, mycelium will become visible & begin to grow.
When mycelium becomes thick, squeeze the nipple right below the top (to close it & protect the polyfil from getting wet) & shake the BB to agitate & break the mycelium into smaller pieces (the marbles help that happen). Then insert a syringe needle & suck out as manyy CC's as you want. Use syringe of liquid culture to inoculate PF, rye or wild bird seed jars. BB mycelium culture will store for a considerable time in the refrigerator.
6T 
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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
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 REDCONTROL
Roadside Raider
Registered: 08/20/02
Posts: 102
Last seen: 15 years, 2 months
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Re: Easy Liquid Culture Container Tek [Re: SixTango]
#1227888 - 01/17/03 10:25 PM (22 years, 12 days ago) |
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Hot dam...i gotta try that...
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The Pope thinks that Charles Darwin is an O.K. dude. Yes, Evolution is a fact, and the Bible has some good truths, but Mushrooms help man to point out the relavant.
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REDCONTROL
Roadside Raider
Registered: 08/20/02
Posts: 102
Last seen: 15 years, 2 months
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Re: Easy Liquid Culture Container Tek [Re: SixTango]
#1227890 - 01/17/03 10:28 PM (22 years, 12 days ago) |
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After you suck the inocced substrate......can you cut it with some water or do you need to ....i think honey might clog the syringe..
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The Pope thinks that Charles Darwin is an O.K. dude. Yes, Evolution is a fact, and the Bible has some good truths, but Mushrooms help man to point out the relavant.
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SixTango
Mycota
Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
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Re: Easy Liquid Culture Container Tek [Re: REDCONTROL]
#1228218 - 01/18/03 05:21 AM (22 years, 12 days ago) |
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LOL, I suggest you use the search function & read up on "liquid culture (as in karo, honey water etc). Only a very small amount of nutrient is diluted in water, then PC'd. 6T
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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
Edited by SixTango (01/18/03 05:24 AM)
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ISH
PsilocybianTranslationServices
Registered: 03/02/02
Posts: 787
Loc: Planet Earth
Last seen: 4 years, 11 months
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Re: Easy Liquid Culture Container Tek [Re: SixTango]
#1229707 - 01/18/03 05:37 PM (22 years, 11 days ago) |
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That's exactly what I was thinking, When I did it I use a bit more, like 1-2 teaspoons of light colored honey. I had mycelium, but not any gigantic mass that I had to deal with, just white fluff in the honey water.
Peace,
ISH
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Join The Shroomery Folding @ Home Team!
I think of going to the grave without having a psychedelic experience, like going to the grave without having sex. It means that you never figured out what it was all about. The mystery is in the body, and the way the body works itself into nature.
- Terence McKenna, Archaic Revival
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SixTango
Mycota
Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
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Re: Easy Liquid Culture Container Tek [Re: ISH]
#1229958 - 01/18/03 08:04 PM (22 years, 11 days ago) |
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If done properly. Myc can get so thick, it will plug a large bore syringe needle. 6T
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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
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ISH
PsilocybianTranslationServices
Registered: 03/02/02
Posts: 787
Loc: Planet Earth
Last seen: 4 years, 11 months
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 Re: Easy Liquid Culture Container Tek [Re: SixTango]
#1230083 - 01/18/03 10:07 PM (22 years, 11 days ago) |
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Damn, mine didn't look that populated, although it did have a lot of the semen like mycelium suspeded in the water.
How much honey did you use in thet, and what strain is it?
Looks awesome.
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Join The Shroomery Folding @ Home Team!
I think of going to the grave without having a psychedelic experience, like going to the grave without having sex. It means that you never figured out what it was all about. The mystery is in the body, and the way the body works itself into nature.
- Terence McKenna, Archaic Revival
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dubyastep
Stranger
Registered: 01/16/12
Posts: 1
Last seen: 12 years, 10 months
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Re: Easy Liquid Culture Container Tek [Re: SixTango]
#15679219 - 01/17/12 04:59 PM (13 years, 10 days ago) |
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Before you pc the baby bottles...Adding about a half inch of dried silicone works best for a self healing injection site.sometimes the syringe will leave multiple holes in the nipple therefore causing air to enter your baby bottle.this tek will prevent the problem from ever occurring.
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