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baja
Stranger

Registered: 04/27/02
Posts: 2
Last seen: 24 years, 6 months

liquid culture using h2o2

#620401 - 04/27/02 02:41 PM (24 years, 6 months ago)

Does anyone see a problem with this method?

1. Using agar techniques, select a good section of mycelium.
2. Put mycelium section in 3% h2o2 premixed with malt extract (4%)
3. Inculate until liquid culture looks complete, then pour over substrate (dung)
4. Continue misting with 3% h2o2

I am about to give step #2 a try and I am wondering if anyone knows if the liquid culture will grow okay in that high a concentration of h2o2. Also, do you think the h2o2 would be enough to keep contams out of the equation?

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francisco
Richman Sporeman

Registered: 01/15/02
Posts: 133
Loc: USA
Last seen: 8 years, 2 months

Re: liquid culture using h2o2

[Re: baja]
#620689 - 04/27/02 09:03 PM (24 years, 6 months ago)

i would at least cut the h2o2 with water 25/75.It really doesn't take that much.

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Well...Maybe just a little.

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john_conner

Registered: 02/22/17
Posts: 243

Re: liquid culture using h2o2

[Re: baja]
#24149870 - 03/09/17 10:51 PM (9 years, 7 months ago)

good to know

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Thedillestpickle
cultured

Registered: 02/02/16
Posts: 1,170
Loc: Canada

Last seen: 6 years, 4 months

Re: liquid culture using h2o2

[Re: john_conner]
#24149916 - 03/09/17 11:08 PM (9 years, 7 months ago)

This doesn't really make sense to me

What's the point of the H202?

Do you plan to pressure cook this liquid with the H202 and malt extract? The H202 will all react with the malt extract and you'll have some extremely oxidized malt extract and no more H202

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PussyFart
Retired Cultivation Extrodinaire

Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 29 days, 13 hours

Re: liquid culture using h2o2

[Re: Thedillestpickle]
#24149922 - 03/09/17 11:11 PM (9 years, 7 months ago)

Why not just ditch the peroxide all together? It serves no real purpose, and is most likely just going to hinder the mycellium from growing...

--------------------

THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE

*** *** AFGHAN KUSH GROW LOG *** ***

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Marmie
Peter peter portion eater

Registered: 10/22/15
Posts: 927
Loc: Deep inside the night
Last seen: 1 year, 2 months

Re: liquid culture using h2o2

[Re: PussyFart]
#24149951 - 03/09/17 11:29 PM (9 years, 7 months ago)

H202 is useless unless used to combat cobweb mold even then it damages the mycelium to, so unless youve got cobweb on ur substrate dont use , and if your mixing it in mea itll react right away and again become useless,
Toss the h202 back under your sink n read some more, theres lots of nifty knowledge to be found on this site

--------------------

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Munchauzen

Registered: 06/23/11
Posts: 14,364

Re: liquid culture using h2o2

[Re: Marmie]

1
#24149972 - 03/09/17 11:37 PM (9 years, 7 months ago)

We quarantined the archives. How are people still coming up with this stuff?

--------------------

All of Munch's Teks!
Munch's YouTube Channel

:suckit:

Munch's Mushroom Tutorial Videos!

:suckit:

Munch's Monotubs with Liners
Blenderless Liquid Inoculate
Agar as Easy as 1-2-3!

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Changa Alchemist
Shwift sauce lifeguard

Registered: 03/17/16
Posts: 2,709
Loc: 90+% Rh

Re: liquid culture using h2o2

[Re: Munchauzen]
#24150005 - 03/09/17 11:51 PM (9 years, 7 months ago)

Munchauzen said:

We quarantined the archives. How are people still coming up with this stuff?

He remembered it from 2002, munch.

:lol:

:lol:

And somehow someone else from 13 years ago comes back to support it. It's the archives fighting back!

--------------------
:ganja:

:ganja:

:mushroom:

:mushroom:

:flyhigh:

:sporedrop:

~~~

:peyote:

:sanpedro:

~~~

:sporedrop:

:chugbeer:

Edited by Shomann (03/09/17 11:54 PM)

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Mad Season
hookers and blackjack

Registered: 09/16/12
Posts: 12,666
Loc: Canada

Re: liquid culture using h2o2

[Re: Munchauzen]
#24150013 - 03/09/17 11:54 PM (9 years, 7 months ago)

Munchauzen said:

We quarantined the archives. How are people still coming up with this stuff?

Look at the op post date.. such an old bump

--------------------

contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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Josh.0
ConnoissurOfSorts

Registered: 11/25/13
Posts: 553

Re: liquid culture using h2o2

[Re: Munchauzen]
#24150014 - 03/09/17 11:54 PM (9 years, 7 months ago)

I think plp still search the site first,not the forums :shrug:

:shrug:

I'm still finding old shit ive never seen before..
And I lurked around for nearly a decade :facepalm:

:facepalm:

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Changa Alchemist
Shwift sauce lifeguard

Registered: 03/17/16
Posts: 2,709
Loc: 90+% Rh

Re: liquid culture using h2o2

[Re: Mad Season]
#24150015 - 03/09/17 11:54 PM (9 years, 7 months ago)

Mad Season said:

Munchauzen said:

We quarantined the archives. How are people still coming up with this stuff?

Look at the op post date.. such an old bump

Thank you. Right over my head.

--------------------
:ganja:

:ganja:

:mushroom:

:mushroom:

:flyhigh:

:sporedrop:

~~~

:peyote:

:sanpedro:

~~~

:sporedrop:

:chugbeer:

Extras:

Munchauzen

Registered: 06/23/11
Posts: 14,364

Re: liquid culture using h2o2

[Re: Changa Alchemist]
#24150023 - 03/09/17 11:56 PM (9 years, 7 months ago)

god damn.

when this op was made, I was a senior in high school :rofl:

:rofl:

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Mad Season
hookers and blackjack

Registered: 09/16/12
Posts: 12,666
Loc: Canada

Re: liquid culture using h2o2

[Re: Josh.0]
#24150024 - 03/09/17 11:57 PM (9 years, 7 months ago)

People need to learn how to use the search function. Here's some pictures to help.

When OP made this I was fuckin 10 years old :shocked:

:shocked:

--------------------

contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras:

Marmie
Peter peter portion eater

Registered: 10/22/15
Posts: 927
Loc: Deep inside the night
Last seen: 1 year, 2 months

Re: liquid culture using h2o2

[Re: Changa Alchemist]
#24150026 - 03/09/17 11:57 PM (9 years, 7 months ago)

Theres more then just this amazing cultivation site on the net , just sucks most of the other ones are so garbage'esk

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3B3B4E
Turd Stool Hunter

Registered: 08/28/16
Posts: 387
Loc: Somewhere I'm not suppose...

Re: liquid culture using h2o2

[Re: Munchauzen]

1
#24150034 - 03/10/17 12:00 AM (9 years, 7 months ago)

He came back to stop Dynacorp
Forgot about the time shift

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If the doors of perception were cleansed everything would appear to man as it is, infinite.
- William Blake

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