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Offlinebaja
Stranger
Registered: 04/27/02
Posts: 2
Last seen: 15 years, 6 months
liquid culture using h2o2
    #620401 - 04/27/02 02:41 PM (15 years, 6 months ago)

Does anyone see a problem with this method?

1. Using agar techniques, select a good section of mycelium.
2. Put mycelium section in 3% h2o2 premixed with malt extract (4%)
3. Inculate until liquid culture looks complete, then pour over substrate (dung)
4. Continue misting with 3% h2o2

I am about to give step #2 a try and I am wondering if anyone knows if the liquid culture will grow okay in that high a concentration of h2o2. Also, do you think the h2o2 would be enough to keep contams out of the equation?


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Offlinefrancisco
Richman Sporeman
Registered: 01/15/02
Posts: 133
Loc: USA
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Re: liquid culture using h2o2 [Re: baja]
    #620689 - 04/27/02 09:03 PM (15 years, 6 months ago)

i would at least cut the h2o2 with water 25/75.It really doesn't take that much.


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Well...Maybe just a little.


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Invisiblejohn_conner
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Registered: 02/22/17
Posts: 67
Re: liquid culture using h2o2 [Re: baja]
    #24149870 - 03/09/17 10:51 PM (8 months, 11 days ago)

good to know


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OfflineThedillestpickle
cultured
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Registered: 02/02/16
Posts: 944
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Re: liquid culture using h2o2 [Re: john_conner]
    #24149916 - 03/09/17 11:08 PM (8 months, 11 days ago)

This doesn't really make sense to me

What's the point of the H202?

Do you plan to pressure cook this liquid with the H202 and malt extract?  The H202 will all react with the malt extract and you'll have some extremely oxidized malt extract and no more H202


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OfflinePussyFartM
Retired Cultivation Extrodinaire
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Re: liquid culture using h2o2 [Re: Thedillestpickle]
    #24149922 - 03/09/17 11:11 PM (8 months, 11 days ago)

Why not just ditch the peroxide all together? It serves no real purpose, and is most likely just going to hinder the mycellium from growing...


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
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EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
  ^*~.,,.~*^*~.,_,.~*^*~.,_SHIT FOR NOOBS_,.~*^*~.,_,.~*^*~.,_,.~* 


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OfflineMarmie
Peter peter portion eater
I'm a teapot User Gallery


Registered: 10/22/15
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Re: liquid culture using h2o2 [Re: PussyFart]
    #24149951 - 03/09/17 11:29 PM (8 months, 11 days ago)

H202 is useless unless used to combat cobweb mold even then it damages the mycelium to, so unless youve got cobweb on ur substrate dont use , and if your mixing it in mea itll react right away and again become useless,
Toss the h202 back under your sink n read some more, theres lots of nifty knowledge to be found on this site


--------------------
Living through a million years of crying, until you realize the art of dying.


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InvisibleMunchauzen
Mushroom Maharaja
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Registered: 06/23/11
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Re: liquid culture using h2o2 [Re: Marmie]
    #24149972 - 03/09/17 11:37 PM (8 months, 11 days ago)

We quarantined the archives. How are people still coming up with this stuff?


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OfflineShomann
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Registered: 03/17/16
Posts: 774
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Re: liquid culture using h2o2 [Re: Munchauzen]
    #24150005 - 03/09/17 11:51 PM (8 months, 11 days ago)

Quote:

Munchauzen said:
We quarantined the archives. How are people still coming up with this stuff?




He remembered it from 2002, munch.

:lol:

And somehow someone else from 13 years ago comes back to support it. It's the archives fighting back!


--------------------
:sporedrop:Growing Tampanensis with Shomann:sporedrop:


Edited by Shomann (03/09/17 11:54 PM)


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 11,733
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Re: liquid culture using h2o2 [Re: Munchauzen]
    #24150013 - 03/09/17 11:54 PM (8 months, 11 days ago)

Quote:

Munchauzen said:
We quarantined the archives. How are people still coming up with this stuff?



Look at the op post date.. such an old bump


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleJosh.0
ConnoissurOfSorts
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Registered: 11/25/13
Posts: 553
Re: liquid culture using h2o2 [Re: Munchauzen]
    #24150014 - 03/09/17 11:54 PM (8 months, 11 days ago)

I think plp still search the site first,not the forums :shrug:
I'm still finding old shit ive never seen before..
And I lurked around for nearly a decade :facepalm:


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OfflineShomann
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Registered: 03/17/16
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Re: liquid culture using h2o2 [Re: Mad Season]
    #24150015 - 03/09/17 11:54 PM (8 months, 11 days ago)

Quote:

Mad Season said:
Quote:

Munchauzen said:
We quarantined the archives. How are people still coming up with this stuff?



Look at the op post date.. such an old bump




Thank you. Right over my head.


--------------------
:sporedrop:Growing Tampanensis with Shomann:sporedrop:


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InvisibleMunchauzen
Mushroom Maharaja
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Registered: 06/23/11
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Re: liquid culture using h2o2 [Re: Shomann]
    #24150023 - 03/09/17 11:56 PM (8 months, 11 days ago)

god damn.

when this op was made, I was a senior in high school :rofl:


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InvisibleMad Season
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Registered: 09/16/12
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Re: liquid culture using h2o2 [Re: Josh.0]
    #24150024 - 03/09/17 11:57 PM (8 months, 11 days ago)

People need to learn how to use the search function. Here's some pictures to help.

   

When OP made this I was fuckin 10 years old :shocked:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineMarmie
Peter peter portion eater
I'm a teapot User Gallery


Registered: 10/22/15
Posts: 427
Loc: Deep inside the night
Last seen: 6 hours, 36 minutes
Re: liquid culture using h2o2 [Re: Shomann]
    #24150026 - 03/09/17 11:57 PM (8 months, 11 days ago)

Theres more then just this amazing cultivation site on the net , just sucks most of the other ones are so garbage'esk


--------------------
Living through a million years of crying, until you realize the art of dying.


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Invisible3B3B4E
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Registered: 08/28/16
Posts: 387
Loc: Somewhere I'm not suppose...
Re: liquid culture using h2o2 [Re: Munchauzen]
    #24150034 - 03/10/17 12:00 AM (8 months, 11 days ago)

He came back to stop Dynacorp
Forgot about the time shift


--------------------
If the doors of perception were cleansed everything would appear to man as it is, infinite.  
- William Blake



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