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Offlinebaja
Stranger
Registered: 04/27/02
Posts: 2
Last seen: 14 years, 7 months
liquid culture using h2o2
    #620401 - 04/27/02 02:41 PM (14 years, 7 months ago)

Does anyone see a problem with this method?

1. Using agar techniques, select a good section of mycelium.
2. Put mycelium section in 3% h2o2 premixed with malt extract (4%)
3. Inculate until liquid culture looks complete, then pour over substrate (dung)
4. Continue misting with 3% h2o2

I am about to give step #2 a try and I am wondering if anyone knows if the liquid culture will grow okay in that high a concentration of h2o2. Also, do you think the h2o2 would be enough to keep contams out of the equation?


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Offlinefrancisco
Richman Sporeman
Registered: 01/15/02
Posts: 133
Loc: USA
Last seen: 13 years, 5 months
Re: liquid culture using h2o2 [Re: baja]
    #620689 - 04/27/02 09:03 PM (14 years, 7 months ago)

i would at least cut the h2o2 with water 25/75.It really doesn't take that much.


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Well...Maybe just a little.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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