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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Last seen: 11 years, 25 days
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: tripndicular]
#3473443 - 12/09/04 09:47 PM (19 years, 1 month ago) |
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There are no x factors if he has done it. I'm asking what he feels his results would be for each jar..each person would get different results but he's been in it long enough to guesstimate.
They have those injection guns on I believe mycosupply, not to expensive either considering time it saves. The blender there is probably more expensive..
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: scatmanrav]
#3474789 - 12/10/04 03:33 AM (19 years, 1 month ago) |
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If you ever want to knock up 100 ++ big grain bags.... Liquid culture & colonizes as fast, if not faster than G2G CHEAP GUNS CLICK HERE CLICH HERE CLICK HERE CLICK HERE CLICK HERE Time to trade the old gun off.
Is not auto refill. Must stick needle in solution to fill & refill by pulling plunger back. But dosage can be set & that is
Edited by agar (12/10/04 06:38 AM)
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GordoSmith
Fatty

Registered: 10/20/04
Posts: 188
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: agar]
#3475581 - 12/10/04 10:51 AM (19 years, 1 month ago) |
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What method do YOU usually use for an incubtaion procedure for lets say.. Pure straw bulk.
-------------------- Wow, those are some fat ass stems.
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Last seen: 11 years, 25 days
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: GordoSmith]
#3475948 - 12/10/04 12:16 PM (19 years, 1 month ago) |
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Holy shit them are cheap! I hadnt found them anywhere other then Mycosupply but turned be away for the time due to its couple hundred dollar price range (it is a nice gun and all but..)..thanks once again agar!
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Alkaloids
3,4,5-trimethoxyphenethylamine


Registered: 11/15/98
Posts: 743
Loc: pubis mons
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: agar]
#3477223 - 12/10/04 03:57 PM (19 years, 1 month ago) |
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Those are very inexpensive. Nice find. Although a decent peristaltic pump with manifold injection ports is what is looking nice right about now.
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tripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: agar]
#3514460 - 12/17/04 04:30 PM (19 years, 1 month ago) |
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BUMPING so others asking these types of questions can re-read this and hopefully learn from it .
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
Edited by tripndicular (12/17/04 04:32 PM)
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MushroomFriend
Vargen ska fanleva!!


Registered: 10/12/04
Posts: 4,055
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: tripndicular]
#3514696 - 12/17/04 05:50 PM (19 years, 1 month ago) |
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LOL just found it! More informative now cause I have more of a agar & liquid mindset atm.. 
Thanks TripnD
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lokiean
Stranger
Registered: 12/10/04
Posts: 30
Last seen: 18 years, 11 months
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: MushroomFriend]
#3514900 - 12/17/04 07:00 PM (19 years, 1 month ago) |
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OK. If i were to spraqy inside a glovebox... (with no filter or fan) and do a grain to grain transfer would it be better then open air room transfers?
Also what kinda contam rate would i be looking at if i were to do Grain to grain transfer to a pastuarized bag of grain??
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
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Loc: Pvt. Pubfag NutSuck
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: lokiean]
#3514918 - 12/17/04 07:05 PM (19 years, 1 month ago) |
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it would be much better than open air transfers, as for failure rates it really depends on you, I'm very clean with my transfers and I may see one in 20 that contam.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: lokiean]
#3515158 - 12/17/04 09:00 PM (19 years, 1 month ago) |
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P-1 is 
I've done them in a VERY CLEAN hepa filtered bathroom, with mask & gloves & done fairly well (1 in 10). Which isn't bad, but still a PITA when you see THIS when you open a jar....... 
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mushroommark
Earning mybluethumb

Registered: 10/01/04
Posts: 359
Last seen: 17 years, 5 months
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: agar]
#3518064 - 12/18/04 07:17 PM (19 years, 1 month ago) |
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Agar, you blow me away time and time again.
Could you write up a tek or some kind of step by step exactly how you make your liquid myc cause I'll tell you mine doesn't look anything like yours lol. Your explanation earlier was an immense help but I'm still curious to hear exactly how you make your slurry. Or at the least what your nutrient medium is.
Cause I just ordered one of the guns, but it won't do me half as good if I don't have some serious growth going on. I've been using dextrose but it never gets that thick. Do you place a hole in the lid for air exchange? Seems like you would but I was always worried that I would get the tyvek wet and invite contams.
Thanks again, your suggestions are always more than welcome.
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mushroommark
Earning mybluethumb

Registered: 10/01/04
Posts: 359
Last seen: 17 years, 5 months
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: mushroommark]
#3518934 - 12/18/04 11:18 PM (19 years, 1 month ago) |
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Also, another question for anybody out there with experience:
If one were so inclined, would it be possible to knock up say one hundred bags, let them colonize, and then refridgerate them until needed for spawning?
This way you could do all your spawning at once and not have to worry about making spawn for a however long they would stay in the fridge. Just I don't know how well the myceliem would be able to recuperate from a long sit in the fridge. Thanks
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cardinalgreen
Cardinal Green
Registered: 12/18/04
Posts: 18
Loc: USA
Last seen: 8 years, 25 days
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: mushroommark]
#3519490 - 12/19/04 02:35 AM (19 years, 1 month ago) |
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YES!! You know your stuff!! Glad your about. I found mushrooms grown outdoors on compost are always stronger in potency than on cakes. Do you find it the same?
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: mushroommark]
#3519710 - 12/19/04 06:38 AM (19 years, 1 month ago) |
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IMHO, a liquid culture must have some degree of gas exchange. The difficulty is HOW TO KEEP A FILTER DRY.

This little gizmo does just that. It is a Kerr/Ball cap, with an IV valve "T". On one "T" is a IV 0.02 micron filter & on the other is a cap.
Simply by turning the "valve" one way or the other, opens or closes a "T" route.
You can asperate jar content via syringe needle straight through the the valve in the upright open position.
Way tired now, so will post the nutrient medium I use, after some sleep.
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kevincoughlin
Stoned

Registered: 07/22/09
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Last seen: 8 years, 7 months
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: agar]
#10928730 - 08/24/09 06:39 PM (14 years, 5 months ago) |
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Can you please reupload the images?
-------------------- Smoke weed every Sunday and throughout your dreams - Jesus 4:20(The one and only God) Their are no stupid questions, just stupid people - Mrs Garrison
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cyb3rtr0n
searching for truth




Registered: 05/06/09
Posts: 1,821
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: kevincoughlin]
#10928789 - 08/24/09 06:47 PM (14 years, 5 months ago) |
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Quote:
kevincoughlin said: Can you please reupload the images?
this thread is 4 years old.
Sometimes people have to delete their pictures stored for one reason or another..
I would just search for more updated info for each part you are looking for. and go by what is written here as a good starting point
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Doctor_Inoc
Vintage Hand
Registered: 04/30/11
Posts: 646
Loc:
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: agar]
#14975153 - 08/25/11 07:16 AM (12 years, 5 months ago) |
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Seen a lot of older posts by expert hands and Trusted Cultivators (of their day) promote the use of liquid culture over agar and grain to grain transfers.
I think if the advice of liquid culture being a faster shredding inoculant than fully colonized grain spawn, as well as agar mediums being to complicated for the beginner, was handed out today, it would be frowned upon.
Done liquid culture for years and in my experience, nothing colonizes a sterile grain medium faster then a 2:10 part ratio of colonized grain spawn to sterilized grains.
The higher percentage of grain spawn to sterile grains results in more, exponential growth.
As mentioned above, it takes at least 5-7 days (if inoculated down the sides of the grain container) for a liquid culture innoc. to reach about 20% colonization before being shook.
With a grain spawn inoculation, you shave off the time it takes to reach this point and, have a grain medium (from time of inoculation) equivalent to a grain jar shook at 20% colonization, fully colonized in said 5-7 days.
However, I can agree that spores mate and myclial culture mobilizes the fastest in liquid nutrient solutions, for these results best match my experience.
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scatmanrav
Brainy Smurf


Registered: 05/08/04
Posts: 11,483
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Last seen: 11 years, 25 days
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: Doctor_Inoc]
#14977277 - 08/25/11 03:30 PM (12 years, 5 months ago) |
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Yeah there was a time when LC were big. Agar helped to popularize them by using stir plates and getting some really nice ones. The good thing about them is in one motion you can take a quart of colonize LC and inject into hundreds of jars.
I do prefer G2G transfers myself. I had some success with LC's then a LOT of failures. G2G almost always leads to success.
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virus1824
Mr Mushroom



Registered: 09/25/05
Posts: 1,751
Loc: Europe
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: scatmanrav]
#14977377 - 08/25/11 03:54 PM (12 years, 5 months ago) |
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LC is a invaluable technique to have. I use it on every grow with great success.
-------------------- A weekend wasted is never a wasted weekend
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soicyboy121
Stranger


Registered: 11/29/11
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Re: Spores, G2G, Liquid Culture, Cloning & Colonization Time. [Re: agar]
#15471907 - 12/05/11 09:40 PM (12 years, 1 month ago) |
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thanks man very helpful with some info for my g2g
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