spliffmasta said:

There is no way that liquid culture is faster than a grain to grain transfer.

1) The myc in a liquid culture is very weak and takes a little bit to recuperate.
2) With G2G you're allowing for more spawn points with stronger, more ready-to-grow myc.

A G2G transfer is almost like starting at the point when you use a liquid culture and give it its first good shake.

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Quote:

scatmanrav said:

Quote:

spliffmasta said:
There is no way that liquid culture is faster than a grain to grain transfer.

1) The myc in a liquid culture is very weak and takes a little bit to recuperate.
2) With G2G you're allowing for more spawn points with stronger, more ready-to-grow myc.

A G2G transfer is almost like starting at the point when you use a liquid culture and give it its first good shake.

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Right and I know agar has said before in a post that G2G is the faster way in his opinion..Im pretty sure he said that..thats why I think that was just mis typed..didnt notice it being mentioned when scanning through responses though so thought I would mention it.

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Grin???..
Okay respected members?? you called me out on this?.

So allow me to explain from my point of view.

G2G is the fastest STRAIGHT FORWARD METHOD.

One step beyond that & not so straight forward is liquid culture.

OPTIMAL liquid culture is the  FASTEST  & here is why (keyword OPTIMAL).

FIRST OFF, everyone?s "liquid culture" RECIPE differs, simply because they use "whatever they can get", that they think & hope will work. Some use Karo (corn sugar syrup), some use honey water, some use light dry malt, some use powdered dextrose, nutritional yeast, potato water, rye water, peptone, soytone, rice water, near beer, combinations of all of the above and/or any number of whatever else comes to mind - after "bong hit 4".

So, all liquid culture solution nutrient mediums ARE NOT EQUAL.

The container type, size & lid everyone uses to hold &  any liquid culture in - ARE NOT EQUAL.

Most just use whatever glass jar & lid that is handy - period. Others use lab grade glassware (or equivalent glassware), with devices to hepa/ulpa filter aerate, stir & aspirate the liquid culture with.

SIZE MATTERS ( & women lie about that - fact)

You obviously get MORE MYCELIUM SOLUTION in a 1 gallon container, than you do in a half pint jar.

So, liquid culture containers DIFFER GREATLY.

INCUBATION:

Some just use a closet shelf & plastic box for incubation. Other use much more regulated incubators.

INCUBATION procedures DIFFER GREATLY.

Then there is what you use to SEED the liquid culture with.

Some use spores, some use agar isolates, some use colonized grains, some use mushroom tissue, etc.

So, there are innumerable VARIBLES in liquid culture recipes, preparation, containers, incubation, aspiration & dispersal methods .

If you use an agar plate isolate to seed liquid culture with, you know it?s clean, mated & you pick the most aggressive growth. That goes into your nutrient solution, is incubated & colonizes.

Under OPTIMAL CONDITIONS mycelium will be exponentially multiply from a tiny fragment, to a very - very large biomass VERY - VERY RAPIDLY.


QUART JAR EXAMPLE


When this is accomplished, the resulting mycelium is so thick, you cannot even aspirate it through a normal 18 gauge syringe needle, as It will instantly PLUG the needle.

You can use LARGE CAPICTY SYRINGES (60 ml - cc) & large bore plastic needles to work out of a jar.

Or use use a self filling syringe system:



Or,  a stirring / cutting mechanism  inside the jar, or a Eberbach type lab blender.





Once done in volume (2+ quart, 2+ liter, per container) you have a massive amount very FINE SLURRY mycelium biomass to inoculate with.

If all is done in OPTIMAL fashion, whatever you inoculate, will colonize FAST - because you are introducing  MILLIONS  of mycelia contact points, rather than hundreds, as in G2G. 

You can even inoculate straight into an OPTIMAL substrate & it will colonize rapidly (but - that is another story).

So, there is a GREAT DIFFERANCE between someone with a half pint jar, with a few wisps mycelia in it, than a 3/4 gallon batch of OPTIMAL mycelia slurry,  thick as a dairy queen milkshake .

kevincoughlin said:

Can you please reupload the images?

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McFinn said:

Reading through this old thread and couldn't pass up the opportunity to tell you how misinformed and stupid your signature is.

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