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Amazon Shop for: ½ Pint Jars, Rye Grain

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OfflineSubmob
Freak of freaks

Registered: 03/01/01
Posts: 48
Loc: here
Last seen: 10 years, 1 month
Help! Liquid culture on grain going bad wrong
    #2038415 - 10/24/03 01:00 AM (13 years, 6 months ago)

this crazy guy at the bar started telling afoaf's roomate about how he had started 2 corn syrup water jars with ecu cubie spores and 2 with philosophers stones. He said he thought the cubes were looking great, with thick ropey mycelium a brewin'. One of the jars of stones (which he dried and soaked in h202 before dropping them in) never did anything, but one appeared to be growing. He shot jars of rye grain on 10/20 from both of the ecu jars and the jar of stones. At this point he handed me a disc. The pictures show the results, all the jars innoculated with apparently healthy ecu cubie mycelium water were thoroughly contaminated with the dread green mold. Here's the greenies



and again



The jars innoculated with the stones show little tiny spots of white growth, but it doesn't look like anything I've come across. Here's one of them



and here's another shot of it



So one wonders, how is it that EVERY jar could have been tainted so badly? He says he tried to keep everything as sterile as possible, though it took a few days after pc'ing them to get them inocculated, and the jars of liquid culture were not refrigerated, but still, wtf? Any help id'ing the contam problem would be much appreciated. P.S. this was all a free-form hallucination, no such persons actually exist, everything you know is wrong


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De Omnibus Dubitandum


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InvisibleMrMaddHatter
Dementia praecox
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Posts: 1,418
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Re: Help! Liquid culture on grain going bad wrong [Re: Submob]
    #2038665 - 10/24/03 02:15 AM (13 years, 6 months ago)

My guess would be that the liquid innoculate was contaminated. Just a guess though :tongue:


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I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.
Hunter S. Thompson

It's always tea-time, and we've no time to wash the things between whiles.

Don't be a fool, this is a war on drugs. Act like. The government sure does!


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Invisibledaussaulit
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Registered: 08/06/02
Posts: 2,894
Loc: Earth
Re: Help! Liquid culture on grain going bad wrong [Re: MrMaddHatter]
    #2038836 - 10/24/03 03:17 AM (13 years, 6 months ago)

What kind of air exchange do you have for the jars? polyfill, tyvek? Did you PC for long enough and at a high enough PSI? Did you soak or boil the grain before? The grain looks dry, you add enough water?


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InvisibleShamanorrhea
Stranger
Registered: 06/20/03
Posts: 304
Re: Help! Liquid culture on grain going bad wrong [Re: Submob]
    #2038899 - 10/24/03 03:45 AM (13 years, 6 months ago)

How did you prepare the rye? It looks far too dry to support any mycelial growth. When I use rye I usually have really fat, glistening berries that stick together.

Here's a tip on determining if a jar is too dry. Shake it and if you can hear the grains rattling it means they are too dry. Try injecting some pre-sterilised water into the jars if they are too dry.


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OfflineSubmob
Freak of freaks

Registered: 03/01/01
Posts: 48
Loc: here
Last seen: 10 years, 1 month
Re: Help! Liquid culture on grain going bad wrong [Re: Shamanorrhea]
    #2044004 - 10/26/03 05:01 AM (13 years, 6 months ago)

the grain was soaked for around 24 hours and the pc'd at about 17 psi for about an hour, they are kinda dry though. Polyfill lids for air exchange.


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De Omnibus Dubitandum


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Amazon Shop for: ½ Pint Jars, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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