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Offlinebillycorgan55
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Registered: 11/14/08
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Re: How to make a liquid cultures FAST (like two days)! [Re: Blue Helix]
    #9431911 - 12/14/08 06:22 PM (15 years, 1 month ago)

what medium did you use?


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Offlineveda_sticks
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Re: How to make a liquid cultures FAST (like two days)! [Re: billycorgan55]
    #9432104 - 12/14/08 07:02 PM (15 years, 1 month ago)

Im glad this post got resurfaced, there is a hell of a lot of good information in there.

Yes theres a way to make agar, using agar agar.

use the search feature.


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Offlinescubabuddha
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Re: How to make a liquid cultures FAST (like two days)! [Re: veda_sticks]
    #9433250 - 12/14/08 09:56 PM (15 years, 1 month ago)

Quote:

veda_sticks said:
Im glad this post got resurfaced, there is a hell of a lot of good information in there.

Yes theres a way to make agar, using agar agar.

use the search feature.



yeah, there is. i didn't realize a stir plate would increase LC performance so much.

can you use a stir plate for more than one container at a time?


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OfflineHippieChick
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Re: How to make a liquid cultures FAST (like two days)! [Re: scubabuddha]
    #9433292 - 12/14/08 10:04 PM (15 years, 1 month ago)

You can buy stir plates with more than one place to place jars



but if the plate is made for one jar , you can't put more than one on it.

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Offlinescubabuddha
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Re: How to make a liquid cultures FAST (like two days)! [Re: HippieChick] * 1
    #9433349 - 12/14/08 10:13 PM (15 years, 1 month ago)

so... if i were planning to make a stir plate, i'd need a spot for each jar?

i feel like there's gotta be a way to make a stir plate for however many jars you could fit on it...


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InvisibleShroominit
Part Time Mycologist


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Registered: 11/29/08
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Re: How to make a liquid cultures FAST (like two days)! [Re: scubabuddha]
    #9434178 - 12/15/08 12:18 AM (15 years, 1 month ago)

scubabuddha, maybe you should look at how they work then.

You take a magnet with N and S poles like (N ========= S) and you put it in the jar (and sterilize and whatever) then put it on the plate. Under the plate you have an electromagnet I'm pretty sure that is a circle O and the electricity creates a magnetic field that circles around it, dragging one pole of the magnet with it, while pushing the other away. Putting two on it  would just make them both shake in place, or jump up the side of a jar (and probably stick to the lid).


Now if you're making one with the computer fan method, you definitely need to use that many fans, etc. If you plan to make an electromagnetic one, it would be awesome and I want the plans, but you could definitely fit a stir spot per jar width on it.


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Offlinescubabuddha
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Re: How to make a liquid cultures FAST (like two days)! [Re: Shroominit]
    #9434249 - 12/15/08 12:31 AM (15 years, 1 month ago)

i've been thinking about it since i posted that and it's definitely do-able. (warning: nerd talk) you need to run inductors in a snaking pattern under the entire plate you plan to use and drive an AC current through them. if you switch the turn direction of every other inductor (so first one is clockwise, the next is counter clockwise, back to CW, etc.) you'll get a uniderictional, alternating magnetic field on the top side... if you put a jar with a stir bar in it anywhere within this field it should work. (end nerd talk)


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OfflineMadR13d
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Registered: 05/16/13
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Re: How to make a liquid cultures FAST (like two days)! [Re: scubabuddha]
    #18324228 - 05/26/13 05:09 PM (10 years, 8 months ago)

I got 3 jars with water and Kayro syrup, mycelium showing growth.  The strains are Reishi, King Oyster, and Pearl Oyster.

Concerning the proper sterile procedure for this when I get these self-healing plugs in the mail...

After you flame heat the syringe till red hot, you just let it cool in the air in front of your hood?

Then do jar to jar/grain transfers? 

Also,  where can you order the filter screw in things?

Thanks,

MadR13d


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OfflineMadR13d
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Re: How to make a liquid cultures FAST (like two days)! [Re: MadR13d]
    #18324242 - 05/26/13 05:11 PM (10 years, 8 months ago)

Oh yeah, and is there some method in Mycology, where they can date how old mycelium is?  Like, one of these really large ones growing in the wild?

Thanks again,  MadR13d


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OfflineJman420
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Registered: 12/23/12
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Re: How to make a liquid cultures FAST (like two days)! [Re: MadR13d]
    #18324247 - 05/26/13 05:12 PM (10 years, 8 months ago)

Quote:

MadR13d said:
I got 3 jars with water and Kayro syrup, mycelium showing growth.  The strains are Reishi, King Oyster, and Pearl Oyster.

Concerning the proper sterile procedure for this when I get these self-healing plugs in the mail...

After you flame heat the syringe till red hot, you just let it cool in the air in front of your hood?

Then do jar to jar/grain transfers? 

Also,  where can you order the filter screw in things?

Thanks,

MadR13d




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