Home | Community | Message Board


MushroomCube.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, 10cc Syringe, Agar, Brown Rice Flour, Malt Extract, Potato Dextrose, Rye Grain, Vermiculite

Jump to first unread post. Pages: 1 | 2 | Next >  [ show all ]
OfflineFungusWarrior
Stranger
Registered: 07/10/04
Posts: 16
Loc: Enchanted Forest
Last seen: 10 years, 8 months
Liquid Culture Difficulties
    #3522788 - 12/20/04 01:39 AM (12 years, 9 months ago)

Three 6 day old PD liquid cultures have so far yielded similar results. A whitish scum formed on the top of the liquid that would droop down at various points into the water. In one jar it settled to the bottom. In another it's still sort of on the top but there's not too much of it. The third jar also had this scum but was shaken vigorously at which point there were chunks of stuff that looked like dead skin floating around in the liquid.

Has anyone experienced this? What's the deal? It looks nothing like the pictures on this site where the mycelium forms clouds in the middle of the solution.


Edited by FungusWarrior (12/20/04 03:00 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Liquid Culture Difficulties [Re: FungusWarrior]
    #3522909 - 12/20/04 02:20 AM (12 years, 9 months ago)

Liquid culture or agar (pda) ?
These two confuse me when used together  :confused:


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFungusWarrior
Stranger
Registered: 07/10/04
Posts: 16
Loc: Enchanted Forest
Last seen: 10 years, 8 months
Re: Liquid Culture Difficulties [Re: tripndicular]
    #3523153 - 12/20/04 03:11 AM (12 years, 9 months ago)

Sorry, called it PDA but it's just potato dextrose liquid (no agar).


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: Liquid Culture Difficulties [Re: FungusWarrior]
    #3523175 - 12/20/04 03:18 AM (12 years, 9 months ago)

Try light dry malt & dextrose (think home brew beer supply store for both).

Level teaspoon of each into quart distilled water.
Simmer on stove to meld solution (don't boil).
Put however much you want in jar, w/filter lid cap.
PC 16 - 18 minutes @ 15 lbs
Allow to cool to room temp in PC.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Liquid Culture Difficulties [Re: agar]
    #3523191 - 12/20/04 03:24 AM (12 years, 9 months ago)

Its about time you came and blessed this post  :wink:


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFungusWarrior
Stranger
Registered: 07/10/04
Posts: 16
Loc: Enchanted Forest
Last seen: 10 years, 8 months
Re: Liquid Culture Difficulties [Re: FungusWarrior]
    #3523209 - 12/20/04 03:30 AM (12 years, 9 months ago)

Might it be that the PD was too concentrated? I'll try to get some pics up soon to show you what it looks like. There's defintely growth of something and I'm fairly sure it's not contams (3 jars displaying similar behaviour, worked in a fairly sterile environment). The growth that is there just seems crippled.

~200g potato was boiled in 500ml water. Strained. Diluted with an additional 500ml and 1tbsp dextrose added.


Edited by FungusWarrior (12/20/04 03:35 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMrFriendly
none
Registered: 10/27/04
Posts: 24
Re: Liquid Culture Difficulties [Re: FungusWarrior]
    #3525961 - 12/21/04 12:34 AM (12 years, 9 months ago)

side question: my pc isn't working, can I just boil liquid culture to sterilize, and how long would be required?

once I notice growth could I just inject some H2O2 to keep bacterial growth from occurring?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKaptKid
Spaced Pirate
Male User Gallery

Registered: 12/11/03
Posts: 6,248
Loc: Bright Side of the Sun
Last seen: 7 months, 5 hours
Re: Liquid Culture Difficulties [Re: MrFriendly]
    #3526147 - 12/21/04 01:25 AM (12 years, 9 months ago)

Quote:

MrFriendly said:
side question: my pc isn't working, can I just boil liquid culture to sterilize, and how long would be required?

once I notice growth could I just inject some H2O2 to keep bacterial growth from occurring?




NO, you need a PC for this.


--------------------
Child of the 60's, Tripping ever since.


:sun:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMrFriendly
none
Registered: 10/27/04
Posts: 24
Re: Liquid Culture Difficulties [Re: KaptKid]
    #3526249 - 12/21/04 01:52 AM (12 years, 9 months ago)

Argh that's not what I wanted to hear....oh well another day. Thanks for info.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 10 months
Re: Liquid Culture Difficulties [Re: agar]
    #3556166 - 12/29/04 07:53 AM (12 years, 9 months ago)

Hi, AGAR in other recipies I see 1 TABLEspoon of Malt on a Cup.
Yours suggest 1 TEAspoon of Malt on a Quart.

Or is the tablespoon on a cup a kind of overkill?


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 9 months
Trusted Cultivator
Re: Liquid Culture Difficulties [Re: MushroomFriend]
    #3557165 - 12/29/04 02:03 PM (12 years, 9 months ago)

He says a teaspoon of both malt and a teaspoon of dextrose to the quart...I'd bet this is the reason..

I did have some incredibly thick growth in my GT culture because I think I used to much karo and I knocked up the culture with like a large teaspoon of colonized rye...I have tons of white growth that floats on the surface but the culture has been successfully used a number of times and isnt contamed at all. I want to try and fruit the culture..I doubt theres enough nutes but even if primordia and small pins start to form thatd be cool..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 10 months
Re: Liquid Culture Difficulties [Re: FungusWarrior]
    #3560427 - 12/30/04 06:36 AM (12 years, 9 months ago)

Yeah also a tsp of dextrose, but still.... In the "teks" I see people having 4% solutions. 10CC (5cc malt, 5cc dextrose) of 1000CC is 1%.

If you succeed in fruiting the culture post me a PIC! :thumbup:

Thanks,
MF


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 9 months
Trusted Cultivator
Re: Liquid Culture Difficulties [Re: MushroomFriend]
    #3560916 - 12/30/04 12:33 PM (12 years, 9 months ago)

I will of course get pics if it does fruit..I've pulled it out of the fridge in the room getting indirect light so we'll see. I'm not expecting anything but this was a topic here before saying it probably couldnt be done because the myc generally wouldnt float at the surface much, even if the nutes are there.. I need a pic of this slurry anyway..thick shat.

By the way...pretty sure quart is only 800cc...its less then a liter...still its a lower concentration but we'll see if agar gets back to us..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 10 months
Re: Liquid Culture Difficulties [Re: FungusWarrior]
    #3561021 - 12/30/04 01:09 PM (12 years, 9 months ago)

Allrighty SMR! :thumbup:

I did my first transfer from brf (agar alternative tek by Doc34) to the malt/dextrose liquid a few days ago.
And it is whosing growth!! Im so happy, my first liquid with growth!

Actually one piece of myc IS floating so I guess it could be fruited too... Wanna try some liquid syringes first though.

I already asked agar in PM but I guess he is to busy. ;P

(according to http://www.paulnoll.com/China/Metric/number-liters.html 1 Quart is 0,94 liter = 940 CC )


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 9 months
Trusted Cultivator
Re: Liquid Culture Difficulties [Re: MushroomFriend]
    #3561053 - 12/30/04 01:17 PM (12 years, 9 months ago)

But the quart jars lines only go up to 800 I think..I dont think he fills it up all the way...well, I dont know much else about it so we'll have to wait for agar to respond...if he PMs you back and doesnt respond here make sure you post back, I'm interested in the answer as well.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 10 months
Re: Liquid Culture Difficulties [Re: FungusWarrior]
    #3561415 - 12/30/04 02:56 PM (12 years, 9 months ago)

Ok will keep you posted if he answers in PM. Dont know now if he meant adding the teaspoons to a quart of water or to a quart jar of water...
But still, 10cc to 800cc still makes only a 1,25% solution.

I cheated a bit and I think I have added 20CC total of malt/dextrose but added some water again too, was so confused.. :laugh:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblethemills
Posts do notindicateIntelligence

Registered: 07/24/04
Posts: 177
Re: Liquid Culture Difficulties [Re: agar]
    #3565858 - 12/31/04 05:57 PM (12 years, 9 months ago)

Quote:

agar said:
Try light dry malt & dextrose (think home brew beer supply store for both).





The home brew strore tells me that both of these things are identical, I once asked for dextrose, and the guy took me to the dry malts and informed me . Could you explain your mixture?
Quality brewing sugars, (glucose, dextrose and malt), seem that they would all, and do have almost the same sugar make-ups seeing as how eithier or may be used. Some information on this site I found interesting
http://www.bodensatz.com/staticpages/index.php?page=Ingredients-FAQ#extract_subst


--------------------
There are 2 possible outcomes: If the result confirms the hypothesis, then you've made a measurement. If the result is contrary to the hypothesis, then you've made a discovery.
-Enrico Fermi
_________________________________________________________________________________________________
Sibling Gatling Gun of Looking at All Sides of the Question


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: Liquid Culture Difficulties [Re: themills]
    #3565986 - 12/31/04 06:37 PM (12 years, 9 months ago)

Dry malt extract is a powder prepared from wheat & barley. The variables that go into producing malt extract range from the variety of wheat / barley used, kilning time and temperature, and how the malted grain is mashed. Basically dry malt is sweet wort reduced to powder formed by removing most of the water by low vacuum evaporation.

Dextrose = alpa-d-glucose is Corn sugar: d-(+)-glucose Molecular formula: C6H12O6


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 9 months
Trusted Cultivator
Re: Liquid Culture Difficulties [Re: agar]
    #3567221 - 01/01/05 02:10 AM (12 years, 9 months ago)

So could you confirm its 1 teaspoon of each for a full quart (being 940ml or 800 or whatever) agar? And is the reason other teks say 1 teaspoon malt per cup because its not dry malt extract?


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: Liquid Culture Difficulties [Re: scatmanrav]
    #3567685 - 01/01/05 07:51 AM (12 years, 9 months ago)

My foaf uses 1 tsp light dry malt, 1 tsp dextrose to about a quart of distilled water (no exacting measurement). He simmers it on the stove (not boil)- to meld the ingredients together.

He then filters that through a coffee filer (to remove any un-dissolved chunks - should any appear, or exist), then he pours the liquid into a quart jar, 2/3rds to 3/4 full & adds a rounded off plate glass shard (as agitator) in the jar. The lid for the jar has a filter on it - to allow for gas exchange & an injection port.

He then PC's jar w/lid & content for 15 - 18 minutes @ 15 lbs & allows it to cool IN PC.

He has injected spores, and/or placed Myc off an agar plate, and colonized grain from G2G into a jar like that & they flourished @ 80 / 84F & agitated every other day.

You could easily double the amount of Malt & Dextrose - if you wanted. Above is just what worked for him. As well as the nutes, he thinks GOOD gas exchange(via filter lid)is important to get THICK Myc in a liquid culture.

Trouble with thick Myc is aspirating it into a syringe via a needle. Real thick Myc tends to plug them. That problem led to him getting stainless steel lab blender (not cheap).

Foaf doesn?t apply exacting science, or very intricate measurements. It?s a fun hobby  & he likes to TINKER. Hell - he is lately having fun fooling around with baby cereal (multi grain, brown rice & potato) flakes as nutrients in agar, slurry cultures & as additives to artificial bulk.

LOL?. He just discovered you can grow one hell of a strong culture out on presoaked PC'ed Couscous :eek: & Acini di pepe :eek: mixed with verm in a baby food jar, 1/2 pint or pint canning jar w/filter lid.

Tinker tinker tinker :wink:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2 | Next >  [ show all ]

Amazon Shop: ½ Pint Jars, 10cc Syringe, Agar, Brown Rice Flour, Malt Extract, Potato Dextrose, Rye Grain, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Liquid Culture Question
( 1 2 all )
folgerswins 2,584 24 09/02/08 10:25 AM
by libertaire
* Problems with PF jars, poo, and liquid culture (HELP!) 4hodmt 704 4 07/09/05 12:28 AM
by backupwards2
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 all )
agar 28,897 38 12/05/11 11:40 PM
by soicyboy121
* Liquid Culture - How to know if it's contaminated Blue Helix 40,813 9 09/29/15 05:47 PM
by Arush
* Muti spore culture difficulty. PsilocybeSamurai 630 5 08/23/05 10:42 PM
by PsilocybeSamurai
* Liquid Culture Challenges Leimana 6,468 4 06/01/05 01:33 AM
by Corporal Kielbasa
* 3 month old liquid culture WBS happyhighbunny 3,512 1 09/22/08 10:59 PM
by scross
* How to make a liquid cultures FAST (like two days)!
( 1 2 3 4 all )
Blue Helix 27,931 69 05/26/13 07:12 PM
by Jman420

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
4,112 topic views. 7 members, 62 guests and 38 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Gaiana.nl
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.006 seconds on 18 queries.