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Failboat
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Nope. The leftovers still seem clean too. I'm prepping a small bit of rye meow to use the extra before I make a new LC. I suppose I ought to prep some agar and streak a plate as well just to see.
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bongoman
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Re: Making Liquid Culture [Re: Failboat]
#26161164 - 08/30/19 05:21 PM (4 years, 4 months ago) |
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A couple of quick questions relating to the Josex biopsy method:
1. He discourages testing LC on agar prior to inoculating it and instead stresses testing the agar culture by poking that on to another agar plate. Thoughts on this? Does anyone here not test their LC on agar before using it? DO you see testing the LC itself as an unacceptable vector?
2. He discourages screw top plastic lids LC vessels but I like the idea of using media bottles which of course are screw top plastic lids.
3. Does LC effectively replace G2G?
Edited by bongoman (08/30/19 05:21 PM)
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verum subsequentis
seeker of truth



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Re: Making Liquid Culture [Re: bongoman]
#26161209 - 08/30/19 05:43 PM (4 years, 4 months ago) |
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i always test my LC on agar before potentially wasting a bunch of grain and energy.
I use screw top lids but wrap them in cling wrap while still in front of the flow.
LC is just a different way of easily expanding mycelium. I wouldn't say it "replaces" G2G. It's just different. Some swear by G2G and some swear by LC. I swear by both
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El Chupacabra
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Hopefully this is good and clean. I streaked a few plates and I inoculated a jar of oats. This is from that beer LC too. I'll keep this updated.
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Failboat
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  PE6 same jar, opposite sides.
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El Chupacabra
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Re: Making Liquid Culture [Re: Failboat]
#26162116 - 08/31/19 09:55 AM (4 years, 4 months ago) |
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I could not be more pissed off right now. I got 4 250ml media bottles and the damn rings on all of them are so loose they're useless. Now I have to wait even longer, and find where the hell they sell those rings for reasonable.
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verum subsequentis
seeker of truth



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you can probably use them without the rings. I pour LC out of a whiskey handle sometimes and those don't have drip rings
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Geinstein
Shroomery addict



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How would one test a LC from a jar without sucking it up a syringe ? Wide mouth jar I don't want to do to much scared I Contam the LC from to opening and closing
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Nothing breads nothing
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mushboy
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Re: Making Liquid Culture [Re: Geinstein]
#26162523 - 08/31/19 02:33 PM (4 years, 4 months ago) |
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Long ass metal inoculation loop.
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verum subsequentis
seeker of truth



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Re: Making Liquid Culture [Re: mushboy]
#26162601 - 08/31/19 03:22 PM (4 years, 4 months ago) |
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yep, inoculation loop for the win. The way i see it, if you aren't good enough with your sterile tek to test an LC without pooping in it, you may be just a tad out of your league messing with liquids. But there's only one way to get better.
I'm going to be testing a bunch of LC later today and was thinking I'd film myself and then put pertinent screenshots up showing how i roll. If so I'll update in my thread later.
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El Chupacabra
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I ended up using all 3 of my 500ml media bottles to use for 3 more batches of LC. I figure by the time I need to make more plates I'll have a few more new 500s to play with.
This time I used 5% beer (Mickeys) for 250 ml of LC broth in all 3 bottles. We'll see how it goes. I couldnt really wait any longer to try these out because I have so many good looking plates ready to go somewhere.. I've been prepping oats for jars and bags for days now. Trying bigger and better shit than ever before.
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Geinstein
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Re: Making Liquid Culture [Re: mushboy]
#26163084 - 08/31/19 10:40 PM (4 years, 4 months ago) |
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Quote:
mushboy said: Long ass metal inoculation loop.

Aaaaaaaaaa sweet man! ! ! ! Although I still like making contact with as little stuff as possible.
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Nothing breads nothing
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nix21
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Re: Making Liquid Culture [Re: Geinstein]
#26164254 - 09/01/19 06:31 PM (4 years, 4 months ago) |
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Hi all.. i know i have to test it on agar, but how this looks to you, ready to use? Also, i noticed after swirling, myc regroup in like 30 sec.. do you guys swirl between every 4 or so jars when pouring?
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Nichrome
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Re: Making Liquid Culture [Re: nix21] 1
#26165010 - 09/02/19 09:29 AM (4 years, 4 months ago) |
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So much fear mongering gets tossed around in here. Just go for it.
Worst case scenario you waste a few hours and a few dollars. You can wait around for days and days for your test plates to "prove" themselves to you (although you are introducing a plethora of new factors) or you can just go for it as time is really all of our most limiting factor. Pouring agar takes time and $ too as well as skill (more time and $) and good plates are an incredible resource.
-------------------- βBetter to be deprived of food for three days, than tea for one.β
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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Failboat
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Re: Making Liquid Culture [Re: Nichrome]
#26165207 - 09/02/19 11:35 AM (4 years, 4 months ago) |
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Sounds like wasting $6 vs $.60.
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mushboy
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Re: Making Liquid Culture [Re: Failboat]
#26165211 - 09/02/19 11:36 AM (4 years, 4 months ago) |
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I personally test my lc on grains
But as a matter of good practice you should always test on agar
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primelines
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Re: Making Liquid Culture [Re: Nichrome] 1
#26165213 - 09/02/19 11:37 AM (4 years, 4 months ago) |
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Quote:
Nichrome said: So much fear mongering gets tossed around in here. Just go for it.
Worst case scenario you waste a few hours and a few dollars. You can wait around for days and days for your test plates to "prove" themselves to you (although you are introducing a plethora of new factors) or you can just go for it as time is really all of our most limiting factor. Pouring agar takes time and $ too as well as skill (more time and $) and good plates are an incredible resource.
Love that post. I didnt test mine. Nocd 10 jars two nights ago and I'm away till tonight. So excited to see what happened good or bad.
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: Making Liquid Culture [Re: mushboy]
#26165217 - 09/02/19 11:40 AM (4 years, 4 months ago) |
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Anyone can reply to this if you know the answer...
Is this the LME that I need to get?
Thanks for any and all replys.
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Failboat
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Re: Making Liquid Culture [Re: YogiBear] 1
#26165228 - 09/02/19 11:44 AM (4 years, 4 months ago) |
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I don't test on agar either, but I have lots of experience making bad LC.
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blackout


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Re: Making Liquid Culture [Re: Failboat] 2
#26165245 - 09/02/19 11:58 AM (4 years, 4 months ago) |
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If you are not going to test it out you can at least put some test wood in the jar prior to sterilizing. I put wooden coffee stirrers in mine, soaked beforehand, you could use ice cream or popsicle sticks. I don't bother testing some of mine that I am confident about, but would use the wood. At least I can get a look at the growth on the stick and know if it is a definite gonner, though of course it is no proper proof that it is good but better than nothing and simple to do.
If there is no growth on the stick you can swirl it about and usually some myc will stick to the wood and then when out of the liquid it will grow fuzzy.
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