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Offlineprimelines
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Re: Making Liquid Culture [Re: Asura]
    #26159844 - 08/29/19 09:07 PM (4 years, 4 months ago)

I knew I'd get told to test on agar...the thought of pouring that liquid onto a plate scares me. I'm for sure going to make the plate wayyy to wet. No? Am I missing something?


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Invisiblenatedawgnow
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Re: Making Liquid Culture [Re: primelines]
    #26159852 - 08/29/19 09:11 PM (4 years, 4 months ago)

Make an inoculation loop, flame sterilize it, dip it into the lc then streak
it to agar


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OfflineEl Chupacabra
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Re: Making Liquid Culture [Re: natedawgnow]
    #26159896 - 08/29/19 09:40 PM (4 years, 4 months ago)

I plan on making a new batch or 2 of LC and using media bottles and pour to inoculate like mushboy shows.
Until then I have a jar with a whatman and a SHIP in the lid. I recently got a bunch of 10ml syringes with shitty slip needles too.
I have regrets.

Would you think using the SHIP and tilting the jar to pull LC, one syringe after another is the best way to go?
Or should I remove the lid and suck it out that way?

I feel like the SHIP is a vector but so is having the lid off long enough to fill 20 syringes, especially with that much hand movements. IDK it's like a pick your poison kinda thing and I just wish I had done it like the OP to begin with.


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Offlineverum subsequentis
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Registered: 03/22/16
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Re: Making Liquid Culture [Re: El Chupacabra]
    #26159911 - 08/29/19 09:55 PM (4 years, 4 months ago)

in the flow i can fill a bunch of needles without introducing a contam. But, your points are valid. Free pouring the lc is the easiest way to avoid these issues in my opinion


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OfflineFailboat
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26160088 - 08/29/19 11:59 PM (4 years, 4 months ago)

I just use an extra media bottle and pour it out directly as though I'm pouring plates. I figure if my plates come out clean my LC<G should too as long as my LC is actually clean. I have 19qt milo I inoculated the other day. Lost one to an accident ripping off a filter, and one jar looks off from the rest, but so far I see myc sans trich.


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Offlineprimelines
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Re: Making Liquid Culture [Re: El Chupacabra]
    #26160258 - 08/30/19 04:46 AM (4 years, 4 months ago)

Quote:

Quirkmeister92 said:
I just use an extra media bottle and pour it out directly as though I'm pouring plates. I figure if my plates come out clean my LC<G should too as long as my LC is actually clean. I have 19qt milo I inoculated the other day. Lost one to an accident ripping off a filter, and one jar looks off from the rest, but so far I see myc sans trich.




I like that idea. Although I dont pour my plates, damn lol.

Quote:

El Chupacabra said:
I plan on making a new batch or 2 of LC and using media bottles and pour to inoculate like mushboy shows.




My girlfriend picked me up non widemouth quart jars a while ago and I havent used them because they look like a pain in the ass. I am going to use them for the jars I'm going to pour the LC into. I figure a bit smaller diameter mouth on the jar would be a tiny bit safer when pouring the LC.


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InvisibleYogiBear
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Registered: 08/24/19
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Re: Making Liquid Culture [Re: Asura]
    #26160268 - 08/30/19 05:15 AM (4 years, 4 months ago)

Quote:

Asura said:
Looks good. But test on a plate to be sure. There is no way anyone can tell you if that is
good just by looking at it. So just be the safest you can be.




I have my first jar going too. That’s good to know. I bought some premade plates and hope they are ok to use. Quick question... How much broth do you put on the plate?


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OfflineAsuraS
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Re: Making Liquid Culture [Re: YogiBear]
    #26160283 - 08/30/19 05:42 AM (4 years, 4 months ago)

Quote:

natedawgnow said:
Make an inoculation loop, flame sterilize it, dip it into the lc then streak
it to agar




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InvisibleYogiBear
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Re: Making Liquid Culture [Re: Asura]
    #26160297 - 08/30/19 05:57 AM (4 years, 4 months ago)

Quote:

Asura said:
Quote:

natedawgnow said:
Make an inoculation loop, flame sterilize it, dip it into the lc then streak
it to agar







Thanks Asura. I totally missed that. I’m a complete rookie to all of this and the forum members have been so kind and helpful.

Thanks for making me a success!


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OfflineEl Chupacabra
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Re: Making Liquid Culture [Re: primelines] * 1
    #26160310 - 08/30/19 06:12 AM (4 years, 4 months ago)

Well primelines a regular mouth jar does seem to pour out much  better than a wide for sure. BUT I went ahead and simulated pouring out of a regular mouth quart jar that just had 250ml of water. I found that it has a tendency to run down the side a bit, which freaks me out.
I also found that if you spin the jar around and pour out so the liquid pours out where the thread is nearest the top (nearest the lip) then the thread kinda acts like a ring of a media bottle and it minimizes the running down the side issue.


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Offlineprimelines
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Re: Making Liquid Culture [Re: El Chupacabra]
    #26160489 - 08/30/19 09:04 AM (4 years, 4 months ago)

Thank you very much for the reply but I have the lc in media bottles, I'm pouring into a  regular mouth wbs jar.


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InvisiblePastywhyteMDiscord
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Re: Making Liquid Culture [Re: primelines]
    #26160498 - 08/30/19 09:10 AM (4 years, 4 months ago)

Quote:

primelines said:
So being a first timer at LC I have to ask the newb question "how does this look".
I just want to know if its ready yet. Made 2 weeks ago.






Those look turbid to me. Be sure to test em.


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OfflineEl Chupacabra
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Re: Making Liquid Culture [Re: primelines]
    #26160507 - 08/30/19 09:15 AM (4 years, 4 months ago)

My bad, totally read that wrong. Got up and got baked this morning.:headbanger:
I only figured that out for myself when I was weighing out my options for getting LC out of my regular mouth jar.

Fuck I can kick myself for not using a media bottle instead.

I just ordered 2 more just for doing LC and when they get here, ITS ON!


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Invisiblenatedawgnow
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Re: Making Liquid Culture [Re: El Chupacabra]
    #26160516 - 08/30/19 09:20 AM (4 years, 4 months ago)

I've been using media bottles and probs won't go back. They work fine even with
no GE hole. I agree with past though, those LCs look a bit cloudy.


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OfflineFailboat
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Re: Making Liquid Culture [Re: natedawgnow]
    #26160616 - 08/30/19 10:38 AM (4 years, 4 months ago)


Got 18 that look clean and just one gnarly fucker. Seems I done well nuff.:awesome:


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: Failboat]
    #26160645 - 08/30/19 11:01 AM (4 years, 4 months ago)

Time will tell. how long ago did you pour those


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OfflineFailboat
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26160658 - 08/30/19 11:10 AM (4 years, 4 months ago)

3 days ago. I could see the growth, both myc and that contam jar yesterday.


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: Failboat]
    #26160663 - 08/30/19 11:14 AM (4 years, 4 months ago)

can i get a few more details? How much LC per jar? did you shake or roll the jars after pouring?


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OfflineFailboat
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Re: Making Liquid Culture [Re: verum subsequentis]
    #26160684 - 08/30/19 11:29 AM (4 years, 4 months ago)

Free poured about 10ml into each jar and shaken after pouring. Have a little leftover after the pours.

This is before the final shake n pours.


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Offlineverum subsequentis
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Re: Making Liquid Culture [Re: Failboat]
    #26160733 - 08/30/19 12:16 PM (4 years, 4 months ago)

tested on agar or nah?


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