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mushboy
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Making Liquid Culture 45
#26022486 - 05/30/19 08:27 AM (4 years, 7 months ago) |
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MAKING LIQUID CULTURE
 
LC should ONLY BE MADE from clean agar plates. this is a point of logic not a 'can it be done' statement. for the sake of making the 10 millionth 'why did i fail?' thread, just take my word and DONT MAKE LCs WITH SPORES.
WHAT IS LC? LC would be the growing culture inside a nutrient rich liquid. the liquid is technically called a broth. this is also the case with agar. so if you see the word BROTH, it simply means the liquid you have prepared. AGAR and LC are the same thing(broths). only agar..has agar WHY LC?because mushrooms HOW TO MAKE THE BROTHmeasure out desired amounts of water. i like doing ~250ml water per broth. you can make gallon LCs if you want  i do smaller amounts because they colonize quicker and 250ml of LC can last a long time. also cuts back on how much media you expose while inoculating and so on.. INOCULATION TEKSJOSEX BIOPSY POKE - mandatory reading  WEDGE METHOD(tek TBA) LETS MAKE THE BROTH  weigh out 1gram of LME(light malt extract..powder!) i like doing ~1g per ~250ml water.   add the powder first THEN add COLD water. this prevents clumping. do NOT use hot water to make broths the hot water can cook the grain powder giving you lumps shake it up good.. sometimes i stash the prepared broth in the fridge for awhile(after grain cycles run) but you dont have too. cover with foil, back off the cap and pc 15min @ 15psi.  liquids sterilize easily. 30min+ can caramelize the sugars/wastes time. i remove from the PC the moment psi hits zero and tighten the cap other pointsunused broths have long shelf lifes. ive let them sit for weeks and they are ready for inoculation when you are. i use unmodified lids. i screw the lid on tight after inoculation. the culture is suspended in liquid and they dont need much GE anyway. NUTE TYPES:you can use many nute sources to make broths. as with agar, the type of nute will determine clarity LIGHT MALT EXTRACT BRF unfiltered GRAIN WATER ive really only made three types. light malt, brf and grain water. LC DISCLAIMERsome people say lc is advanced or contamination prone ect.. IME this is not correct. however, LC can and will exploit any cracks in your cultivation armour. grains prepped too wet? cultures not entirely clean? SAB technique not down?? youll grow nothing but green real truthhoney/sugary LCs are so 1995
Edited by mushboy (05/30/19 08:59 AM)
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mushboy
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Re: Making Liquid Culture [Re: micelio] 6
#26022670 - 05/30/19 10:03 AM (4 years, 7 months ago) |
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i just pour them and eye ball it.
250ml of lc is enough for at least 25 jars. 50 if you got skills. i usually get about 30ish but im lazy like that.
i dont shake after inoculation either. just let them chill. i need to know if i contaminate from the liquid or from technique. impossible to tell if you shake shit up right away.
this means you did it correctly
 
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mushboy
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Re: Making Liquid Culture [Re: micelio] 1
#26022740 - 05/30/19 10:41 AM (4 years, 7 months ago) |
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the only times ive had the grains get too wet is if i prepped them kinda wet
OR, if i use the lc before its ready(colonized). like this one.
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mushboy
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Re: Making Liquid Culture [Re: Camera93] 1
#26022753 - 05/30/19 10:46 AM (4 years, 7 months ago) |
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i dunno. never done it before.
but i made this little cake by dumping some lc ontop but it took awhile to colonize.
 
id imagine the syringe injected lc would be better suited but aspirating into syringes is a different tek and requires modded lids.
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Camera93
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Nice!! Okay cool, Ill find some lid teks, Quote:
ShaperDreaming said:
Quote:
Camera93 said: Really wanna get going on some Mudda style grows and it looks like LC/LI is the best inoculation method
I've been messing around with Mudda bottles and grains, and let me just tell you that is NOT the route to go with them.
I started getting into LC simply because I want to keep doing mudda bottles for gourmet work and grains fucking suck with them. I have ideas on how to make grains work... but why when I can just pour in some LC. I'm a week or so out from my next test (I have 400ml of LC growing out right now, about 60% done), and I'll be pouring from media bottles (same as this tek essentially). I think, if anything, the small amount of extra hydration will be helpful for mudda bottles (the zip-lock twist kind).
Yeah grains in the bottle is tough, I just did the same but didn't supplement and PC because I was pretty certain I was gonna make a mess in the SAB trying to pour the grain. I made a hole in the center of 4 pints and poured some grain, the 5th pint i just mixed it all up like i do for cubes/coir that one has been the fastest by far. Sub is just lipa teked fuel pellets, redistributed to the pints
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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ShaperDreaming
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Re: Making Liquid Culture [Re: Camera93] 1
#26022817 - 05/30/19 11:37 AM (4 years, 7 months ago) |
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Ahhh, I realize I'm talking the same shit in two threads.
Quote:
Camera93 said: I made a hole in the center of 4 pints and poured some grain, the 5th pint i just mixed it all up like i do for cubes/coir that one has been the fastest by far.
Yeah, the dude from FreshCap Mushrooms shows using a wooden spoon handle to create a hole in the center before pc, then pouring into that hole:

This was my next idea... but still you can't shake/distribute it. I feel like the best option would be to do LC and the hole in the center, the give it a mean shake after you close the lid (then unscrew it a 1/4 turn for GE).
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sandman420
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Same thing...they are designed to seal by pressing the top of the jar on the bottom of the lid. The threads only exist to apply pressure on this area, not to make a seal. It is GL-45 threads of you want to google it if you don't trust me. Here is some pictures one of one of my GL-45 caps, this is a 3 piece GL-45 cap you can see it has a o ring that sits on the underside of the metal part that the plastic top presses on to seal it. Not all Gl-45 caps have the o-ring, just this particular type. But they are still designed to make their seal on the underside of the lid like literally every small capped liquid container in existence. Water bottles jugs etc...
Edited by sandman420 (06/14/19 08:40 AM)
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ShaperDreaming
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Re: Making Liquid Culture [Re: sandman420]
#26051901 - 06/14/19 08:32 AM (4 years, 7 months ago) |
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Huh... I thought those weird caps (GL-45 or something like that?) were designed specifically to not create a 100% seal even in that case. Damn.
More on topic: I pulled the trigger on a stirplate and got 2 - 250ml media bottles... but I'm cheap and found the cheapest one. The fucking measure lines weren't even level and the damn drip-rim thing wouldn't stay on (you can see a gap in the image)

Returning them today, ordering other ones. I figure that if I'm going to do this I may as well do it right and get the right vessels. 500ml is just a little too big, these will be perfect and I can just PC them with the stir sticks in and trade off on the plate.
I think I'll stick with the cling-wrap over the cap and ignore the plumbers tape.
I also got that cheap ass stir plate, and it works but it's not great at low speeds (stalls out)... still. It works.
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ShaperDreaming
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Re: Making Liquid Culture [Re: sandman420]
#26051916 - 06/14/19 08:40 AM (4 years, 7 months ago) |
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HUH! That must be nice. All of my media bottles are sans gaskets like this:
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Bph
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Re: Making Liquid Culture [Re: LtLurker]
#26132858 - 08/12/19 07:59 PM (4 years, 5 months ago) |
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So how do you know when the LC is ready to use? Got one that I feel confident is clean but not sure if it's fully colonized.

Will it take over all the water and become all myc or am I just waiting for the water to clear up. (which it has become noticably so)
Edited by Bph (08/12/19 08:02 PM)
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Bph
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Re: Making Liquid Culture [Re: Bph]
#26132877 - 08/12/19 08:18 PM (4 years, 5 months ago) |
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Kind of a bad pic maybe this is better.
Edited by Bph (08/12/19 08:19 PM)
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Bph
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Re: Making Liquid Culture [Re: Pastywhyte]
#26133068 - 08/12/19 10:52 PM (4 years, 5 months ago) |
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I have tested it last week. It just took off slowly so I figured it was not quite ready. Honestly I'm just scared I will mess it up trying to test it on agar. How do you test LC that you planned to pour?
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Bph
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Re: Making Liquid Culture [Re: Failboat]
#26133081 - 08/12/19 11:04 PM (4 years, 5 months ago) |
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It's a lot of LC. Lol. I love me some oats just still have like 100# of bird seed so I have been mixing them in 50/50.

Figure I can get like 20+ bags out of this.
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mushboy
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Re: Making Liquid Culture [Re: primelines]
#26133423 - 08/13/19 08:29 AM (4 years, 5 months ago) |
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 although i only make ~250ml broths. kinda wasting the other space. maybe i should get smaller bottles for lc and save the 500s for agar
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Stipe-n Cap


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Having a smaller opening would be preferable, but the mason setup is super convenient for obvious reasons in every other way. Might try my old erlenmeyer flask next time, I have the foam plugs for the opening.

I've been using them in my media bottles for agar, prevents boil over and acts as a filter.
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mushboy
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Quote:
nix21 said: for you to see full colonization on 250ml LC?
about a week or two. depends. the poke takes slightly longer because you are starting with tiny fragments opposed to a wedge.
Quote:
verum subsequentis said:
It is said sometimes that a colony floating means your lc is fubared but i have seen evidence that suggests otherwise.
a floater is what im looking for colonies that settle are usually fucked ime
a healthly floater

some lazy turd
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mushboy
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Quote:
verum subsequentis said: i thought he was talking about a colony that is literally growing on the surface of the liquid. From my observation, floating vs sinking can be caused by a few things. Namely, contams in the liquid/ culture or amount of oxygen in the liquid/ culture.
Ah I misread. Yah I agree with you. Colonies on the surface usually are bad.
Like this..
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Tweeq
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Nice write up! If I may propose another simple recepy. For those of us who regularly eat boiled potatoes: mix some of the potato water that is left after boiling with some regular tapwater to get very clear LC
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mushboy
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Re: Making Liquid Culture [Re: LtLurker]
#26133876 - 08/13/19 02:14 PM (4 years, 5 months ago) |
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Also use long ass loops.

I dont use stir bars because I dont need lc under a deadline.
Edited by mushboy (08/13/19 02:33 PM)
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Bph
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Re: Making Liquid Culture [Re: mushboy]
#26133888 - 08/13/19 02:21 PM (4 years, 5 months ago) |
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Quote:
LtLurker said: tilt your jar. Then you don't need to be above it with your hand and handle.
Ahhhhh. Tilt the jar.
Quote:
mushboy said: Also use long ass lopes

I dont use stir bars because I dont need lc under a deadline.
That is a long ass loop. Now I can see how it all works. Gonna go rig me up something out of bailing wire. Nothing like getting your questions answered. Thanks everyone.
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