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Re: AGAR ENVY! (Anything and All things agar!) [Re: ImpishShroom]
    #25703541 -

mushroomnate said:
Mike O.Rrhizal said:
For Christmas I got these 100x40mm PP5 plates for no pour agar the same size as a Petri dish.

Cant wait to get them growing!



Be sure to update us on these.:takingnotes:



Will do :thumbup:
ImpishShroom said:
Did a grain to Agar for my fastest growing Qt.

Time to start isolating πŸ–€
First time, always taking advice.

Agar, Corn meal, honey, royal jelly





Looks like you have a nice spot of fast growth in the bottom left dish


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mike O.Rrhizal]
    #25703622 -

Someone sent me a couple of APE swabs.  I have never used swabs before.  On  December 14, I smeared the one on two plates then just put the swab in another.  Nothing is happening on the two and the third with the actual swab just looks like mold.
Did I use it right?  I did save the second swab so I still can try again.



I realize I have a lot of condensation, tried pouring hot as someone else said they do and it did not work out well lol. Going back to pouring when it reaches about 45-48.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25703629 -

You just streak the swab like you would a loop: I've seen people pull the cotton off with APE bc the spores are clear... idk lol


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πŸ„πŸ’šπŸ˜Ž

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25703640 -

Hmmm.  There was a dark spot on the swab, but you say the spores are clear?

I don’t mind waiting a bit longer for the streaked plates, just thought I should see some sign by now, 12 days.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25703654 -

Swabs can last a long time. I’ve seen some ppl drop them on plates, but you could do a hundred swipes before you retire it like that. Condensation can be tricky, but shouldn’t kill your plate if your SAB technique is good. Just try not to get the swab too wet when streaking it. Practice opening the swab in a way that you can set it down on the foil while the swab is still being tented by it in between plates.
You will get your culture going :thumbup: you could try to transfer away from that mold for some practice. The right side looks possibly workable


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"Once your spawn jars are fully colonized, you can scratch your butt while you inoculate the coir if you want."-RR

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sporealchemy]
    #25703657 -

APE is a true albino, and thus has no pigment whatsoever: even the spores


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πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25703683 -

Thanks @sporealchemy and Caps!  My plates aren’t usually full of condensation lol experiment gone wrong. 

I’ll try a transfer and use the other swab too.  When I’m done I’ll take a pic of it, maybe they sent the wrong one?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25703736 -

Idk... maybe


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πŸ„πŸ’šπŸ˜Ž

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25703780 -

APE swabs will have a dark or blue tint or color for sure.I don't think it's the spores that do it tho.


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BRF to bulk/Cakes as spawn

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushroomnate]
    #25703922 -


Well... I guess I can't pour plates? I waited longer this time, but still a lot of humidity... How do ya'll know when it's cool enough to pour? I tried resting a kitchen thermometer against the jar this time, but that's not very accurate I guess :frown:

What you're not seeing here is my Rusty White spores taking off! Yes! Both plates seem to have success!

Also, my PE transfers are taking off! They seem to like their new plates and are thickening up their myc.

My golden oyster LC2agar is ugly! I think there was a ton of junk in that live culture. Because of the condensation it's hard to see if anything good is happening in there, but I do see a lot of mycelium in there... I'm going to let it keep going fo a while.

I also learned that I'm not as sterile as I would like to be. After flaming my scalpel and cooling it in the agar, I can see nasty shit growing off the carbon it left behind. I guess I'm learning why agar is important for getting clean spawn.



First transfer to grains from agar (PE) is going well, both jars inoculated and we have take off!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25703958 -



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Last seen 12/2/2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Duhrwood Stevens]
    #25703974 -

I pour when I can hold the media bottle comfortably, bare handed... sometimes, the bottom has started to coagulate, and the last couple of dishes end up a little thick... stack them as high as I can in the SAB... usually only the top 2 plates of each stack have condensation, and very little, which clears up before I use them typically


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πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25704004 -

Duhrwood Stevens said:
Get a point&shoot IR thermo, they're cheap.



They are, damn!
Caps McGee said:
I pour when I can hold the media bottle comfortably, bare handed... sometimes, the bottom has started to coagulate, and the last couple of dishes end up a little thick... stack them as high as I can in the SAB... usually only the top 2 plates of each stack have condensation, and very little, which clears up before I use them typically



So, I'm doing it by when I can hold it in my bare hand... I cook a lot... Maybe my hands are broken?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25704046 -

140f is cool enough to hold but still 20+ degrees too hot. Compare it to coffee, sippin' temp is different than chuggin' temp. If it feels like 'last chance' coffee it's perfect.
With a thermo I've found 120f is gonna steam up way more than 116 but you run out of time fast at 116. Thick agar is worse to thicken up while pouring than soft IMO. :goodluck:


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Last seen 12/2/2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Duhrwood Stevens] * 1
    #25704062 -

I'm talking comfortably... for normal folks: shower temp lol


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πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25704086 -

Caps McGee said:
I pour when I can hold the media bottle comfortably, bare handed... sometimes, the bottom has started to coagulate, and the last couple of dishes end up a little thick... stack them as high as I can in the SAB... usually only the top 2 plates of each stack have condensation, and very little, which clears up before I use them typically



I do this exactly with the same results!:thumbup:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dabadoodledoo710]
    #25704090 -

RW clone, T3:



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πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25704110 -

Looking good caps.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MH5109]
    #25704118 -

All stays well, prep more oats in a few days... got 14 qts RW, 4 qts PE6, and 2 quarts Texas going already... got the bug bad after thanksgiving/not cultivating anything but plates for like 6 weeks


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πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MH5109]
    #25704125 -

Couple CRS. Anyone know what the circular lines are from? Still grows out fine, just curious.

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