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OfflineRandyFeller
EagerBeaver
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Registered: 12/18/18
Posts: 185
Loc: PNW Flag
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25709924 - 12/29/18 02:43 PM (5 years, 30 days ago)

Poured on the 18th. Not too thin but I did leave the plates sitting in my SAB for 24 hours before I transferred and wrapped em. Maybe they got a little too dry? After 10 years away, everything makes me paranoid as far as contams go.

Thank you Caps!

We need a Caps Critique my Culture thread.


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OfflinePyrotek
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Registered: 06/02/18
Posts: 44
Last seen: 4 years, 3 months
Re: AGAR ENVY! (Anything and All things agar!) [Re: ithikuss]
    #25709993 - 12/29/18 03:11 PM (5 years, 30 days ago)

This pic is a few days old so it's grown out a bit but I decided to get some good practice in with this culture. I took 20 total transfers from this one little guy. Might have stretched it a little too thin but I wanted some extra plates to mess around with. I used a couple different ratios with the nutrients in the agar so I'm looking forward to seeing what affect this has. I'll post the results once they grow out a bit.



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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: RandyFeller]
    #25709998 - 12/29/18 03:16 PM (5 years, 30 days ago)

Quote:

RandyFeller said:
Poured on the 18th. Not too thin but I did leave the plates sitting in my SAB for 24 hours before I transferred and wrapped em. Maybe they got a little too dry? After 10 years away, everything makes me paranoid as far as contams go.

Thank you Caps!

We need a Caps Critique my Culture thread.



Lol, idk about all that... but I don't think you've got contamination there: just some bruising IMO


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OfflineFungeyes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25710343 - 12/29/18 06:18 PM (5 years, 30 days ago)



How long should I PC swabs. My plan is to take one and swipe a print so I can transfer to Caps Mcgee's soft agar recipe.


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InvisibleR.I.P.Zappa
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Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Fungeyes] * 1
    #25710354 - 12/29/18 06:22 PM (5 years, 30 days ago)

Do em with your next agar run. 30 mins should do. :cookiemonster:


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OfflineFungeyes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: R.I.P.Zappa]
    #25710468 - 12/29/18 07:13 PM (5 years, 30 days ago)

Thanks for the quick response working on my no pour lids atm


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OfflineNaildrvr
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Registered: 12/17/17
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Fungeyes]
    #25710579 - 12/29/18 08:42 PM (5 years, 30 days ago)

My favorite agar pic. My first PE clone. Wish i cloned a cluster, lol


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OfflineNaildrvr
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Registered: 12/17/17
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Naildrvr]
    #25710584 - 12/29/18 08:46 PM (5 years, 30 days ago)

Damit.


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OfflineThesunbeam
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Registered: 07/08/18
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Naildrvr]
    #25710602 - 12/29/18 09:11 PM (5 years, 30 days ago)

Clone that little guy


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Thesunbeam]
    #25711147 - 12/30/18 07:37 AM (5 years, 29 days ago)

Quote:

Thesunbeam said:
Clone that little guy



:snoopyes:
I PC swabs at 15psi for 20 minutes  (after venting)


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OfflinePyrotek
Stranger
Registered: 06/02/18
Posts: 44
Last seen: 4 years, 3 months
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25711220 - 12/30/18 08:37 AM (5 years, 29 days ago)

I know why you transfer the newest and quickest growing mycelium but what are the drawbacks from transferring a piece closer to the center? Is it bad mostly because of unseen contaminants that could be lurking or Does the mycelium not grow as quickly since it's not the newest? And a little update- so far none of the 20 transfers I took the other night are showing any signs of contams. I'll be happy if I get 10 healthy cultures out of the bunch.


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InvisibleCaps McGee
Grandaddy Smurfshack
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Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Pyrotek]
    #25711279 - 12/30/18 09:30 AM (5 years, 29 days ago)

Quote:

Pyrotek said:
I know why you transfer the newest and quickest growing mycelium but what are the drawbacks from transferring a piece closer to the center? Is it bad mostly because of unseen contaminants that could be lurking or Does the mycelium not grow as quickly since it's not the newest? And a little update- so far none of the 20 transfers I took the other night are showing any signs of contams. I'll be happy if I get 10 healthy cultures out of the bunch.



:snoopyes:
BOTH actually... bacteria can easily grow under the culture and be undetectable to the untrained eye... also, the culture at the leading edge of growth is in search of food, so is more vigorous than the established (feeding and lazy) growth near the center... not that it won't colonize,  just more efficient and much safer from the leading edge


--------------------
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Ask Caps! (My Q&A Thread)
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OfflineFungeyes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25711317 - 12/30/18 09:47 AM (5 years, 29 days ago)

Thanks Caps McGee


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Fungeyes]
    #25711327 - 12/30/18 09:54 AM (5 years, 29 days ago)

:rockon::rockon::rockon:


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Ask Caps! (My Q&A Thread)
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Offlineblazedup
Dirk Diggler
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Registered: 06/05/11
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25711436 - 12/30/18 11:03 AM (5 years, 29 days ago)

Been dabbling in agar again. Thoughts?



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OfflineMike O.Rrhizal
Tree
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Registered: 12/02/18
Posts: 482
Loc: Native Soil Flag
Last seen: 2 years, 8 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup]
    #25711447 - 12/30/18 11:11 AM (5 years, 29 days ago)

Looking good blaze :thumbup:


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InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh πŸ˜›
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Registered: 07/01/17
Posts: 6,067
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mike O.Rrhizal]
    #25711477 - 12/30/18 11:33 AM (5 years, 29 days ago)

Sharing some plates of "AH FUCK!"  :mypleasure:

Relatively easy to grow once it starts.  :facepalm3:

:lol:


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InvisibleBumholio
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Registered: 07/23/18
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25711482 - 12/30/18 11:38 AM (5 years, 29 days ago)

Might need to do a transfer or 2 there Torm.


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InvisibleTormatoMFacebookDiscordReddit
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bumholio]
    #25711503 - 12/30/18 11:55 AM (5 years, 29 days ago)

Quote:

Bumholio said:
Might need to do a transfer or 2 there Torm.



:lol:


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Helpful Threads

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Tormato's Q&A Thread Post Questions Here or PM me!

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InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25711668 - 12/30/18 02:16 PM (5 years, 29 days ago)

Quote:

Tormato said:
Sharing some plates of "AH FUCK!"  :mypleasure:

Relatively easy to grow once it starts.  :facepalm3:

:lol:




delicious


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