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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
#25735465 - 01/10/19 08:02 AM (5 years, 18 days ago) |
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Just tomentose
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Humanhelper
Stranger

Registered: 12/18/18
Posts: 224
Loc: South by Southwest
Last seen: 4 years, 6 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
#25735528 - 01/10/19 08:44 AM (5 years, 18 days ago) |
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Yep. Just happy it threw out some fuzz in the last twenty four hours. The three soup dishes I have going didnโt get me super inspired.
-------------------- It looks a lot more like it does now than it did...
 
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Tormato  
The Goddess Kali Meh ๐




Registered: 07/01/17
Posts: 6,067
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
#25735764 - 01/10/19 10:56 AM (5 years, 18 days ago) |
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Quote:
Humanhelper said: First go at agar...glad this thread is here. MOSTLY FOR THE LAUGHS, partly for the knowledge and tips.
It's for learning and feedback, but casual like.
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Humanhelper
Stranger

Registered: 12/18/18
Posts: 224
Loc: South by Southwest
Last seen: 4 years, 6 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
#25735784 - 01/10/19 11:10 AM (5 years, 18 days ago) |
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It was the โanus envyโ that made me laugh the most. May have lost a little coffee out the nose when I read it.
-------------------- It looks a lot more like it does now than it did...
 
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Tormato  
The Goddess Kali Meh ๐




Registered: 07/01/17
Posts: 6,067
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
#25735811 - 01/10/19 11:24 AM (5 years, 18 days ago) |
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Quote:
Humanhelper said: It was the โanus envyโ that made me laugh the most. May have lost a little coffee out the nose when I read it.
It's the shit!
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Momโs Rabbit Hole
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
#25735925 - 01/10/19 12:26 PM (5 years, 18 days ago) |
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Quote:
Tormato said:
Quote:
Humanhelper said: It was the โanus envyโ that made me laugh the most. May have lost a little coffee out the nose when I read it.
It's the shit! 
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TEKs I Like
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
#25735926 - 01/10/19 12:27 PM (5 years, 18 days ago) |
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Or gives you the shits... hell I can't remember
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Humanhelper
Stranger

Registered: 12/18/18
Posts: 224
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Last seen: 4 years, 6 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
#25735952 - 01/10/19 12:39 PM (5 years, 18 days ago) |
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Either way....itโs the shit, or shits!
Question: whatโs up with my specimens going translucent on agar. Is the condensation problem previously discussed at fault, or is this normal. I would load a pic, but due to the condensation issue you canโt see shit, not even anus envy. The previous pic of tomentose growth was translucent prior to going white and I see no problems but I have 5 more dishes (3 of which are just cell soup in top of the agar) that have all gone pretty translucent.
-------------------- It looks a lot more like it does now than it did...
 
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Tormato  
The Goddess Kali Meh ๐




Registered: 07/01/17
Posts: 6,067
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
#25735963 - 01/10/19 12:42 PM (5 years, 18 days ago) |
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Quote:
Humanhelper said: Question: whatโs up with my specimens going translucent on agar. Is the condensation problem previously discussed at fault, or is this normal. I would load a pic, but due to the condensation issue you canโt see shit, not even anus envy. The previous pic of tomentose growth was translucent prior to going white and I see no problems but I have 5 more dishes (3 of which are just cell soup in top of the agar) that have all gone pretty translucent.
IME that's usually a nute issue.
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
#25735967 - 01/10/19 12:43 PM (5 years, 18 days ago) |
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I see this with high levels of LME
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Humanhelper
Stranger

Registered: 12/18/18
Posts: 224
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Last seen: 4 years, 6 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
#25736026 - 01/10/19 01:11 PM (5 years, 18 days ago) |
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Sounds good. Iโve learned pretty extreme patience so Iโll record whatโs happening, keep an eye on it, and then compare to future agar mixtures. I did a pretty stout PDA mix at 12-14%. Thanks
-------------------- It looks a lot more like it does now than it did...
 
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
#25736090 - 01/10/19 01:44 PM (5 years, 18 days ago) |
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That's super strong!
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Humanhelper
Stranger

Registered: 12/18/18
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
#25736190 - 01/10/19 02:45 PM (5 years, 18 days ago) |
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I agree after reading through this entire thread. Iโll try out different concentrations and once Iโve worked my way through this 100g of PDA Iโll start mixing up some of my own. I wanted stout for better coring of samples to avoid scalpe work so I went with what Iโve done in the lab. Shit youโd almost think I was doing gel electrophoresis:)
-------------------- It looks a lot more like it does now than it did...
 
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TXBD
StrangerthanFiction


Registered: 06/24/18
Posts: 261
Loc: Tejas
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Humanhelper]
#25736476 - 01/10/19 04:42 PM (5 years, 18 days ago) |
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Here's a couple AA+ cultures 
 And some close ups


And some nastiness on an APE plate. My transfers made it out OK 

Some PF Redspore T2 that I bounced across the dishes

And a closer look

I'm thinking I shouldn't even transfer an agar cutting to new dishes.. just touch the new plate and move on.. I seem to knock my cutting around when I wrap them up. Cause I'm cool like that! ugh!!
-------------------- I overdo everything and nothing at the same time. Every time, All the time, and some times too..
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: AGAR ENVY! (Anything and All things agar!) [Re: TXBD]
#25736535 - 01/10/19 05:11 PM (5 years, 18 days ago) |
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You've got a bacterial satellite at 1030 first AA+ plate... I beat my plates up too: have an AA+ (I think) where i dropped it, the wedge is stuck to the lid now! It's colonizing and it's clean...
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
#25736546 - 01/10/19 05:16 PM (5 years, 18 days ago) |
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Forgot pictures! Cultures are as labeled:
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 1 day
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Re: AGAR ENVY! (Anything and All things agar!) [Re: TXBD]
#25736548 - 01/10/19 05:17 PM (5 years, 18 days ago) |
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Well, I was a bit off today and stayed home so I decided cook up some new agar since I wasn't super happy with my last batch. This is 400+ml water, 6g agar, 6g lme, and 4 drops green food color. It looks super clear!
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TXBD
StrangerthanFiction


Registered: 06/24/18
Posts: 261
Loc: Tejas
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
#25736572 - 01/10/19 05:24 PM (5 years, 18 days ago) |
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Quote:
Caps McGee said: You've got a bacterial satellite at 1030 first AA+ plate... I beat my plates up too: have an AA+ (I think) where i dropped it, the wedge is stuck to the lid now! It's colonizing and it's clean...
Yeah, I'm transferring out of these plates. I remember being in a hurry with the SAB work that day. I got a lot done but there were certainly more mistakes made. I just hope I didn't screw up any grain jars too!
That's OK though. More practice! I'm going to hit it again tonight
-------------------- I overdo everything and nothing at the same time. Every time, All the time, and some times too..
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: AGAR ENVY! (Anything and All things agar!) [Re: TXBD]
#25736691 - 01/10/19 06:11 PM (5 years, 18 days ago) |
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That's what's up... strength in numbers (of spawn jars lol)
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Humanhelper
Stranger

Registered: 12/18/18
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Last seen: 4 years, 6 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
#25737602 - 01/11/19 06:40 AM (5 years, 17 days ago) |
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Quote:
ShaperDreaming said:

Well, I was a bit off today and stayed home so I decided cook up some new agar since I wasn't super happy with my last batch. This is 400+ml water, 6g agar, 6g lme, and 4 drops green food color. It looks super clear!
I like whatever it is you do...โum, why arenโt you at work today?โ...โoh, I called in โa bit off.โโ
Post updates of how your agar performs. Iโm at training wheel stage and would like to keep up with different mixtures and performances. Hope you feel less off soon...
HH
-------------------- It looks a lot more like it does now than it did...
 
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