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OfflineTormatoS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25662469 - 12/06/18 09:15 PM (3 months, 13 days ago)

Quote:

Caps McGee said:
EXPANSION!





Nice stack!
:amusedapplause:


--------------------
Tormato's Q&A Thread

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"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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InvisibleR.I.P.Zappa
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #25662471 - 12/06/18 09:15 PM (3 months, 13 days ago)

Cool looking plate of a Burma clone I took out a couple days ago, put it in cold storage back in july.



Tidal Wave ready for transfer


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OfflineTormatoS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: R.I.P.Zappa]
    #25662500 - 12/06/18 09:23 PM (3 months, 13 days ago)

Quote:

R.I.P.Zappa said:
Cool looking plate of a Burma clone I took out a couple days ago, put it in cold storage back in july.







I love how the myc is growing around those pockets...that's awesome!!


--------------------
Tormato's Q&A Thread

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"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

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InvisibleJHOVAS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25662560 - 12/06/18 09:56 PM (3 months, 13 days ago)

Quote:

Tormato said:
Quote:

JHOVA said:




Swab?



pray it germs. this shit hasnt the last 2 times i swiped it :crankey: its fuckin stuck in that bitch.


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OfflineTormatoS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: JHOVA]
    #25662582 - 12/06/18 10:02 PM (3 months, 13 days ago)

Quote:

JHOVA said:
Quote:

Tormato said:
Quote:

JHOVA said:




Swab?



pray it germs. this shit hasnt the last 2 times i swiped it :crankey: its fuckin stuck in that bitch.



What agar mix are you using?


--------------------
Tormato's Q&A Thread

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"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

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OfflineSumFunGi
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Re: AGAR ENVY! (Anything and All things agar!) [Re: JHOVA]
    #25662599 - 12/06/18 10:05 PM (3 months, 13 days ago)

What do you guys think of plastic Petri dishes already filled with agar and sterile like they use in medical labs?  I found a place to get them.  Didn’t know if they would have the same nutrients needed for myc.  Any thoughts?  Sleeve of 20 already ready to go sounds
Good to me.

And if I have a laminar flow hood, how important is the “lab”.  Won’t the work done under the hood be good and clean ( provided it’s done right).?  Ie, I don’t need to hyper clean everything since work will be done under the hood?


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Onlinetripdawg420
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SumFunGi]
    #25662604 - 12/06/18 10:07 PM (3 months, 13 days ago)

Quote:

SumFunGi said:
What do you guys think of plastic Petri dishes already filled with agar and sterile like they use in medical labs?  I found a place to get them.  Didn’t know if they would have the same nutrients needed for myc.  Any thoughts?  Sleeve of 20 already ready to go sounds
Good to me.

And if I have a laminar flow hood, how important is the “lab”.  Won’t the work done under the hood be good and clean ( provided it’s done right).?  Ie, I don’t need to hyper clean everything since work will be done under the hood?



no pour in a sab is what i do pretty easy and cheap


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OfflineGrimsweeper
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 3
    #25662636 - 12/06/18 10:18 PM (3 months, 13 days ago)


Not bad for a germ plate especially from a swab. The satellites look funky in the pic but I think they're clean colonies in 1st person.

Another killer germ plate from Spinlymass, thanks bro.



Just in case...



Keeping media bottles at exactly 117F with a Sous Vide



Pastys in cold storage


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OfflineTormatoS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grimsweeper]
    #25662649 - 12/06/18 10:22 PM (3 months, 13 days ago)

Dude! I have got to get me one of those media warmers... that's bad ass bro!


--------------------
Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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OfflineGrimsweeper
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25662659 - 12/06/18 10:26 PM (3 months, 13 days ago)

Quote:

Tormato said:
Dude! I have got to get me one of those media warmers... that's bad ass bro!



Takes the rush out of pouring, I love it. Great for small batch pasteurization too. Bought my bud one for Christmas, tell your significant other. AND... it'll cook the best steak you ever had.


--------------------
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Onlinetripdawg420
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25662669 - 12/06/18 10:30 PM (3 months, 13 days ago)

Quote:

Tormato said:
Dude! I have got to get me one of those media warmers... that's bad ass bro!



that reminds me of a stinger when they took hot pots out the prisons lol


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflineTormatoS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tripdawg420]
    #25662675 - 12/06/18 10:33 PM (3 months, 13 days ago)

Quote:

Grimsweeper said:
Quote:

Tormato said:
Dude! I have got to get me one of those media warmers... that's bad ass bro!



Takes the rush out of pouring, I love it. Great for small batch pasteurization too. Bought my bud one for Christmas, tell your significant other. AND... it'll cook the best steak you ever had.



Oh I'm sold! :lol:



Quote:

tripdawg420 said:
Quote:

Tormato said:
Dude! I have got to get me one of those media warmers... that's bad ass bro!



that reminds me of a stinger when they took hot pots out the prisons lol



Oh shit it does!

:nowaydude:


--------------------
Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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OfflineStroph
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25662855 - 12/07/18 12:22 AM (3 months, 13 days ago)

Got a print of Ps. Ovoideocystidiata today courtesy of Hobbyist (thank you, my friend) and it just hit agar tonight. Nothing to see yet, but hopefully. Great thread! :thumbup:


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OfflineTormatoS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stroph]
    #25663135 - 12/07/18 07:10 AM (3 months, 13 days ago)

Quote:

Stroph said:
Got a print of Ps. Ovoideocystidiata today courtesy of Hobbyist (thank you, my friend) and it just hit agar tonight. Nothing to see yet, but hopefully. Great thread! :thumbup:



Post pics when you can!

:fuckyeah:


--------------------
Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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OfflineSunnyDayzeS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25663300 - 12/07/18 10:02 AM (3 months, 13 days ago)

I am new to LME agar.  The first attempt worked well, the second I cracked the lid when their was pressure and it sucked in contams, made me want to cry as I didn’t give it too much thought and a few days later every damn plate was ruined.  This time I thought I was so careful but all my plates look messed.  Is it the agar?  Is it contams?  Is it ok?  I was going to throw them out when I saw the weird swirly stuff but decided to watch for a bit to see what happens.

This one I didn’t germinate.



This one was a transfer.



They all pretty much look like that.  Is it just sediment?  Need to go back to potato or GW lol

Edit:  Tormato, I love this thread idea!  Good thinkin!


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OfflineDarkyDonno
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25663309 - 12/07/18 10:12 AM (3 months, 13 days ago)

Well I just got my first isolate of Orissa India, I think I did 4 clones to agar and 3 have obvious sectoring but the fourth is a perfect circle. I transferred to two more plates so hopefully I can use it by next week. Will post pics when I'm home.


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OfflineTormatoS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25663377 - 12/07/18 10:56 AM (3 months, 13 days ago)

Quote:

SunnyDayze said:
I am new to LME agar.  The first attempt worked well, the second I cracked the lid when their was pressure and it sucked in contams, made me want to cry as I didn’t give it too much thought and a few days later every damn plate was ruined.  This time I thought I was so careful but all my plates look messed.  Is it the agar?  Is it contams?  Is it ok?  I was going to throw them out when I saw the weird swirly stuff but decided to watch for a bit to see what happens.

This one I didn’t germinate.



This one was a transfer.



They all pretty much look like that.  Is it just sediment?  Need to go back to potato or GW lol

Edit:  Tormato, I love this thread idea!  Good thinkin!



If it's not moving around or growing I would say it's just sediment...it happens :shrug:

Thank you! We got so much information all over the site, but my hope is that this thread will bring everything together to one thread. :cheers:


--------------------
Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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OfflineDuhrwood Stevens
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25663422 - 12/07/18 11:32 AM (3 months, 13 days ago)

Might be caramelized sugar, how long did you pc? Over 20-25 minutes makes it happen to me.
It doesn't hurt anything.
If you put food coloring in, 10 drops or more per liter, it's easier to see some contam stuff and you can focus on what's on the agar rather than in it.
Good luck. :mushroomgrow:  :mushroomgrow:  :mushroomgrow:


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//files.shroomery.org/files/18-46/229612825-100_1609.jpg][/url]_SX425_.jpg[/image][/url]


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OfflineSunnyDayzeS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Duhrwood Stevens]
    #25663442 - 12/07/18 11:50 AM (3 months, 13 days ago)

I pc’d for 25 minutes but my rubber plug wasn’t sitting right and it took a long time to get up to pressure.  I do have a couple of drops of red fc in there but not much.  Interesting.  I’ll keep watching but the mycelium looks good and unaffected by whatever sediment or caramelization.  I did not add sugar, does LME naturally have it?


--------------------
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Invisibleithikuss
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25663445 - 12/07/18 11:56 AM (3 months, 13 days ago)

Tried something similar a bit back, but my name doesn't have as much weight to get attention starting a thread..,lol https://www.shroomery.org/forums/showflat.php/Number/25613817/page/1

Anywho, surprised how clean this is so far straight from the swab..

which gave this,


And some F9 RW;




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