Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Kratom Powder For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < First | < Back | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinelordfurd
Stranger
Registered: 11/28/18
Posts: 5
Last seen: 1 year, 6 months
Re: AGAR ENVY! (Anything and All things agar!) [Re: Grimsweeper]
    #25729206 - 01/07/19 02:26 PM (5 years, 21 days ago)

I'm growing on agar from MS syringe just to make sure there's no contams before transferring to 4 quart bulk jars, would it be better to transfer 4 small wedges from the same petri that is growing the fastest/cleanest or mix it up with multiple dishes or does it not matter?


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: lordfurd]
    #25729227 - 01/07/19 02:36 PM (5 years, 21 days ago)

Clean culture... source is irrelevant


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
OfflineBeefSupremeJr
Detritivore
Male User Gallery

Registered: 11/02/11
Posts: 6,812
Loc: 29.9792Β° N, 31.1342Β° E Flag
Last seen: 4 hours, 28 minutes
Trusted Cultivator
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25729320 - 01/07/19 03:11 PM (5 years, 21 days ago)

If the dishes are all from the same syringe it doesn’t matter I don’t think,  but I don’t like the idea of spawning germs plates.

Aside from not being able to properly spot contams on a germ plate, there’s also always weak myc that I don’t want anything to do with. 

Uniformity. Consistency.


Extras: Filter Print Post Top
OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 2 days, 3 hours
Trusted Cultivator
Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefSupremeJr]
    #25729332 - 01/07/19 03:18 PM (5 years, 21 days ago)

I took a transfer from a clone plate of a plate pin. I dont care for my "pastyplates " and I'm gonna order a case of 500 petris in the next week. I'm gonna get another transfer deep then start expanding with multiple transfers till I can tic tac toe a few dozen pints.


Extras: Filter Print Post Top
InvisibleSunnyDayze
Finder of shrubberies
Female User Gallery


Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Grimsweeper]
    #25729404 - 01/07/19 03:45 PM (5 years, 21 days ago)

Quote:

Grimsweeper said:
I'll post this here since it seems I've beat my battle with reoccurring condensation on petri cultures and can hopefully help others. Lots of good input from others and an eventual fix.




I was having condensation issues too and it definitely was temperature swings for me.  I have wood heat stove in the house so temperature fluctuation is bad.  I cranked up an electric heater in the room so it is more consistent.  Hoping that cures it, helping so far.


--------------------


Extras: Filter Print Post Top
Offlinelordfurd
Stranger
Registered: 11/28/18
Posts: 5
Last seen: 1 year, 6 months
Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefSupremeJr]
    #25729411 - 01/07/19 03:49 PM (5 years, 21 days ago)

Yeah I should have taken a clone of a good fruit from my last grow but only just getting into agar so I'll do that next time. I figure it's got to be at least a little safer for contams than shooting the solution into the jars.


Edited by lordfurd (01/07/19 03:50 PM)


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: lordfurd]
    #25729560 - 01/07/19 04:44 PM (5 years, 21 days ago)

It's multitudes safer/ cleaner than MS inoculation


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
InvisibleBigSurMoon
the deathless ones
 User Gallery


Registered: 03/21/18
Posts: 876
Loc: Chicken coop
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25729608 - 01/07/19 05:07 PM (5 years, 21 days ago)

Does my ape cultures look okay?


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: BigSurMoon]
    #25729777 - 01/07/19 06:04 PM (5 years, 21 days ago)

Gonna play a little; going to attempt to transfer these 2 entire RW cultures away from the bacterial colonies near the edges... they're clean otherwise



And taking a transfer from this PE6 clone slanted in July....



--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 8 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25730611 - 01/08/19 12:03 AM (5 years, 21 days ago)

thinkin about trying grain soak agar for the first time. I'm not seeing any specifics out there, so I'm guessing nutritional potency of the water doesn't really matter? Just boil some grain in water and put agar in it, no fuss?


Extras: Filter Print Post Top
OfflinePhosCap
Gratuitous Heavenly Grace
 User Gallery


Registered: 06/09/10
Posts: 975
Loc: Tartary
Last seen: 8 months, 12 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: Psilosopherr]
    #25730712 - 01/08/19 01:35 AM (5 years, 21 days ago)

Agar transfer soon. 7 to 4 o'clock range.



--------------------
Psychoactive Fungi of the World        TLs            Franks teks and Tips        Stonesun's..
Spitballs Quick Rye Prep


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: PhosCap]
    #25730986 - 01/08/19 07:17 AM (5 years, 20 days ago)

I've seen grain soak diluted up to 1:4... I'd start with 50/50 and see what type of growth you get

I'd take 3 small transfers from 4-7... a good bit ahead of the rest of the culture, and looks like it's going to go rhizomorphic... nice pick, and nice pic!


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
OfflineFishLevelMidnight
Aquaman
Male User Gallery


Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 23 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee] * 1
    #25730997 - 01/08/19 07:23 AM (5 years, 20 days ago)

Yeah I always make my GW from at least 6-8 quarts of grain, no straining or anything.

I freeze what I don’t use (mix first to homogenize sediment) in ~300mL aliquots.

I then mix 200-250 mL of the grain water with my agar and fill to 500 mL for GWA.

I don’t like it full strength, but that may be due to how concentrated mine is to begin with


--------------------





Trade List





Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: FishLevelMidnight]
    #25732047 - 01/08/19 05:04 PM (5 years, 20 days ago)

Let's do it to it...



--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
OfflineBeefSupremeJr
Detritivore
Male User Gallery

Registered: 11/02/11
Posts: 6,812
Loc: 29.9792Β° N, 31.1342Β° E Flag
Last seen: 4 hours, 28 minutes
Trusted Cultivator
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25732688 - 01/08/19 08:57 PM (5 years, 20 days ago)

Quote:

Caps McGee said:
Let's do it to it...







Looks like β€œsagar”

What was your mix this time around?


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefSupremeJr]
    #25733292 - 01/09/19 07:40 AM (5 years, 19 days ago)

3.5g agar powder and 6.5g LME to 500ml of water


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
OfflineBeefSupremeJr
Detritivore
Male User Gallery

Registered: 11/02/11
Posts: 6,812
Loc: 29.9792Β° N, 31.1342Β° E Flag
Last seen: 4 hours, 28 minutes
Trusted Cultivator
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25733376 - 01/09/19 08:25 AM (5 years, 19 days ago)

I am going to try it when I actually have a clone I like. 
What would you use for malt extract?  I’m thinking about 15g per 1000ml.


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefSupremeJr]
    #25733393 - 01/09/19 08:37 AM (5 years, 19 days ago)

I use 6.5g LME (light malt extract) to 500ml of water... so 13g per liter... but as much as 20g is standard MEA... just play around with different nute levels with different cultures: see what gives you the type of growth you like


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh πŸ˜›
I'm a teapot User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 6,067
Trusted Cultivator
Re: AGAR ENVY! (Anything and All things agar!) [Re: Psilosopherr] * 1
    #25733450 - 01/09/19 09:04 AM (5 years, 19 days ago)

Quote:

Psilosopherr said:
thinkin about trying grain soak agar for the first time. I'm not seeing any specifics out there, so I'm guessing nutritional potency of the water doesn't really matter? Just boil some grain in water and put agar in it, no fuss?



That's how I do it. I capture the grain water when I strain grains and then add agar powder to the grain water (10g agar/500ml grain water) per how much agar I plan to make. I also use the grain water to make LC as well. Rye seems to work best IMO.


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Extras: Filter Print Post Top
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 1 day
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25733506 - 01/09/19 09:36 AM (5 years, 19 days ago)

Quote:

Tormato said:
Quote:

Psilosopherr said:
thinkin about trying grain soak agar for the first time. I'm not seeing any specifics out there, so I'm guessing nutritional potency of the water doesn't really matter? Just boil some grain in water and put agar in it, no fuss?



That's how I do it. I capture the grain water when I strain grains and then add agar powder to the grain water (10g agar/500ml grain water) per how much agar I plan to make. I also use the grain water to make LC as well. Rye seems to work best IMO.



Still experiementing with this myself. I'm thinking Rye would get better results than oats because of the hull. Here's from my most recent pour:



You can see the grainy bits floating around. My first plates were crystal clear... got down a little further in the jar and here it is.

Also, I'm starting to think that 10g agar is too much for low temp pours. I noticed that I was pouring solid bubbles trapped in the agar (you can see in these pics too) and I poured at 120f. I'm going to drop my agar down to 7g or less.

I bought some LME and I'm going to make that next time I pour agar. Just to see what I like. Here's my next recipe for 500g I'm going to work with:
  • 7g LME
  • 7g Agar

Sound like a good test to folks?


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | Next > | Last >

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Kratom Powder For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* buying agar - help sinisha 3,416 6 03/31/06 05:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,382 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,155 8 02/25/05 11:03 AM
by agar
* test tubes agar fungophiliac 3,134 8 06/19/20 09:41 PM
by gorilliam
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 4,587 12 03/30/20 10:37 AM
by Surf707
* Agar and the global economy Dem_Bones 1,650 4 03/01/06 09:08 AM
by Dem_Bones
* Agar question WormholeSurfer 1,482 3 10/29/05 07:10 PM
by agar
* Agar's Home Made Compost recipe for bulk substrates
( 1 2 3 4 all )
agar 20,874 75 02/12/21 05:50 AM
by Bart84

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
540,822 topic views. 11 members, 146 guests and 50 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.009 seconds on 13 queries.