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InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh 😛
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Re: AGAR ENVY! (Anything and All things agar!) [Re: J. Jack Flash]
    #25711671 - 12/30/18 02:21 PM (5 years, 29 days ago)

Quote:

J. Jack Flash said:
Quote:

Tormato said:
Sharing some plates of "AH FUCK!"  :mypleasure:

Relatively easy to grow once it starts.  :facepalm3:

:lol:




delicious



:lol:


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OfflinePyrotek
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25712015 - 12/30/18 05:34 PM (5 years, 29 days ago)

What am I looking at in this top Petri dish? Am I correct in stating that the only healthy mycelium is in the center of the bottom plate?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pyrotek]
    #25712047 - 12/30/18 05:51 PM (5 years, 29 days ago)

Quote:

Pyrotek said:
What am I looking at in this top Petri dish? Am I correct in stating that the only healthy mycelium is in the center of the bottom plate?



Who mine?


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Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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OfflineMH5109
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25713137 - 12/31/18 04:34 AM (5 years, 28 days ago)

Some maz jars the top one was inoculated 12/17 and the bottom one was 12/22.



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OfflinePyrotek
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25713190 - 12/31/18 06:03 AM (5 years, 28 days ago)

Quote:

Tormato said:
Quote:

Pyrotek said:
What am I looking at in this top Petri dish? Am I correct in stating that the only healthy mycelium is in the center of the bottom plate?



Who mine?




Yes


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InvisibleCaps McGee
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pyrotek]
    #25713264 - 12/31/18 07:42 AM (5 years, 28 days ago)

I wouldn't call any of that healthy mycelium


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InvisibleBumholio
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25713350 - 12/31/18 08:47 AM (5 years, 28 days ago)

I poured 20 plates on Christmas eve and when I made my agar I put it in the PC in 2 bottles. A Frank's red hot bottle and a smaller encona hot sauce bottle (because I knew there wasnt enough for all 20 in just the Frank's bottle.) After the PC run I took both bottle out the PC and could hear hissing coming from the encona bottle, FUCK!! I hadn't tightened the lid properly and it was sucking in dirty air.

I guessed what was coming next as I'd read Mateah say it'd happened to his grain jars a few weeks before and the got contaminated. I poured them anyway and kept the 4 plates separate from the Frank's plates.

Had a look today and all 4 are contaminated where as all 16 Frank's plates are fine.

Moral of the story is that in the short time it took for me to take the bottle out of the PC and tighten the lid it was able to draw in contams that were able to colonize these plates, and itll happen with grain jars LCs or whatever else isn't properly filtered or tightened :shrug:




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InvisibleTormatoMFacebookDiscordReddit
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25713541 - 12/31/18 10:30 AM (5 years, 28 days ago)

Quote:

Caps McGee said:
I wouldn't call any of that healthy mycelium



:whathesaid: Top one is some kind of green mold and the bottom is a bacteria colony.


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OfflineMike O.Rrhizal
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25713664 - 12/31/18 11:25 AM (5 years, 28 days ago)

Update on petri sized PP5 pots:

So I used Josex's No pour tek and PDA agar for these large PP5 containers so far with mixed success. I noticed there was a lot more condensation inside the plates when they came out the PC, I always have condensation with the smaller pots with no adverse effects. The larger pots seem to have attracted more bacterial growth for some reason.

Heres a transfer that has just grown bacteria:


Some success:
South American multispore looking fluffy


Blue oyster has grown aggressively


Shiitake getting started


Notice the large droplets of water compared to the beaded up water drops normally inside smaller no pour agar pots.


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OfflinePyrotek
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25714310 - 12/31/18 04:38 PM (5 years, 28 days ago)

Quote:

Caps McGee said:
I wouldn't call any of that healthy mycelium



Just from reading other comments I thought I was missing something. Some of the comments made me think they were supposed to look like that. I obviously didn't pick up on the sarcasm. Good to know I'm not crazy. Was thinking to myself, "what kind of mycelium is this?" I'm not the brightest.


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OfflineRandyFeller
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pyrotek]
    #25714333 - 12/31/18 05:00 PM (5 years, 28 days ago)

Had a few extra plates and decided to do a little New Years Eve tomfoolery.





No expectations, just having fun.


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OfflineShaperDreaming
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Re: AGAR ENVY! (Anything and All things agar!) [Re: RandyFeller]
    #25716375 - 01/01/19 01:37 PM (5 years, 27 days ago)

Lots of fun agar work going on right now!


This seems like pretty aggressive breakdown back to some really strong rhyzomorphic growth. I'm super excited. It came from the biggest guy in that Golden teacher grow.



I redid my golden oyster plate. This time I used a sterile inoculation loop... Which was not as easy to use as I hoped. Anyways, I have cleaner signs of growth here now. Still very thin mycelium.


My PE transfers seem to be growing really strong! I'm super excited to have isolated good sectors it seems.


So this last one is from an APE swab I got. I wiped it pretty good along the surface, there's some contam in the bottom left corner... But then... Maybe mycelium from the APE near the bottom center-right? I zoomed in a little too. Any thoughts?


Edited by ShaperDreaming (01/01/19 01:43 PM)


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OfflineFungeyes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25718776 - 01/02/19 01:16 PM (5 years, 26 days ago)



Few question agar noobie here:
So I've swabbed my thai print and transferred over to agar in my S.A.B.  roughly how soon should I start watching for grow? Got em in a room that's 73f. While I was at it I swabbed 3 plates of RW thanks to Caps McGee. 1 plate of TC syringe that is old as balls and probably should of started off on brf do to bacteria then transfer to agar, but we'll see where that goes.


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InvisibleJohnny Dont
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Fungeyes]
    #25718843 - 01/02/19 01:37 PM (5 years, 26 days ago)

You should see growth within 3 days up to 3 weeks or so


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Offlineblazedup
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Johnny Dont]
    #25719411 - 01/02/19 05:19 PM (5 years, 26 days ago)

First time using a inoc loop with a print i received in a trade. This is ovoid.



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OfflineFailboat
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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup]
    #25719582 - 01/02/19 06:19 PM (5 years, 26 days ago)

Just cloned 4 TLY from plates, dropped a pin in the SAB and still tossed it on a pasty lol gonna have some tams


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InvisibleTormatoMFacebookDiscordReddit
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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup]
    #25719588 - 01/02/19 06:20 PM (5 years, 26 days ago)

Quote:

blazedup said:
First time using a inoc loop with a print i received in a trade. This is ovoid.





Looks good my man! :thumbup:


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Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

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OfflineFungeyes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mike O.Rrhizal]
    #25719812 - 01/02/19 08:18 PM (5 years, 26 days ago)

Hey by chance you notice how loose the lids seem? I picked some up to I'm hoping it ain't a issue.


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Offlineblazedup
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25719846 - 01/02/19 08:33 PM (5 years, 26 days ago)

Quote:

Tormato said:
Quote:

blazedup said:
First time using a inoc loop with a print i received in a trade. This is ovoid.





Looks good my man! :thumbup:




Appreciate it


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Offlineblazedup
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Fungeyes]
    #25719869 - 01/02/19 08:46 PM (5 years, 26 days ago)

Quote:

Fungeyes said:
Hey by chance you notice how loose the lids seem? I picked some up to I'm hoping it ain't a issue.



I noticed that. Have to be careful with them


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