Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMike O.Rrhizal
Tree
I'm a teapot

Registered: 12/02/18
Posts: 482
Loc: Native Soil Flag
Last seen: 2 years, 8 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: ImpishShroom]
    #25703541 - 12/26/18 10:21 AM (5 years, 1 month ago)

Quote:

mushroomnate said:
Quote:

Mike O.Rrhizal said:
For Christmas I got these 100x40mm PP5 plates for no pour agar the same size as a Petri dish.

Cant wait to get them growing!



Be sure to update us on these.:takingnotes:



Will do :thumbup:
Quote:

ImpishShroom said:
Did a grain to Agar for my fastest growing Qt.

Time to start isolating πŸ–€
First time, always taking advice.

Agar, Corn meal, honey, royal jelly





Looks like you have a nice spot of fast growth in the bottom left dish


--------------------


Extras: Filter Print Post Top
InvisibleSunnyDayze
Finder of shrubberies
Female User Gallery


Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Mike O.Rrhizal]
    #25703622 - 12/26/18 11:14 AM (5 years, 1 month ago)

Someone sent me a couple of APE swabs.  I have never used swabs before.  On  December 14, I smeared the one on two plates then just put the swab in another.  Nothing is happening on the two and the third with the actual swab just looks like mold.
Did I use it right?  I did save the second swab so I still can try again.



I realize I have a lot of condensation, tried pouring hot as someone else said they do and it did not work out well lol. Going back to pouring when it reaches about 45-48.


--------------------


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25703629 - 12/26/18 11:17 AM (5 years, 1 month ago)

You just streak the swab like you would a loop: I've seen people pull the cotton off with APE bc the spores are clear... idk lol


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
InvisibleSunnyDayze
Finder of shrubberies
Female User Gallery


Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25703640 - 12/26/18 11:22 AM (5 years, 1 month ago)

Hmmm.  There was a dark spot on the swab, but you say the spores are clear?

I don’t mind waiting a bit longer for the streaked plates, just thought I should see some sign by now, 12 days.


--------------------


Extras: Filter Print Post Top
Invisiblesporealchemy
ok


Folding@home Statistics
Registered: 04/10/11
Posts: 401
Loc: ( Ν‘Β° ΝœΚ– Ν‘Β°)
Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25703654 - 12/26/18 11:30 AM (5 years, 1 month ago)

Swabs can last a long time. I’ve seen some ppl drop them on plates, but you could do a hundred swipes before you retire it like that. Condensation can be tricky, but shouldn’t kill your plate if your SAB technique is good. Just try not to get the swab too wet when streaking it. Practice opening the swab in a way that you can set it down on the foil while the swab is still being tented by it in between plates.
You will get your culture going :thumbup: you could try to transfer away from that mold for some practice. The right side looks possibly workable


--------------------
"Once your spawn jars are fully colonized, you can scratch your butt while you inoculate the coir if you want."-RR


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: sporealchemy]
    #25703657 - 12/26/18 11:33 AM (5 years, 1 month ago)

APE is a true albino, and thus has no pigment whatsoever: even the spores


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
InvisibleSunnyDayze
Finder of shrubberies
Female User Gallery


Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25703683 - 12/26/18 11:43 AM (5 years, 1 month ago)

Thanks @sporealchemy and Caps!  My plates aren’t usually full of condensation lol experiment gone wrong. 

I’ll try a transfer and use the other swab too.  When I’m done I’ll take a pic of it, maybe they sent the wrong one?


--------------------


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25703736 - 12/26/18 12:06 PM (5 years, 1 month ago)

Idk... maybe


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
Offlinemushroomnate
Pstranger
 User Gallery


Registered: 05/17/17
Posts: 3,100
Last seen: 13 hours, 56 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25703780 - 12/26/18 12:33 PM (5 years, 1 month ago)

APE swabs will have a dark or blue tint or color for sure.I don't think it's the spores that do it tho.


--------------------


BRF to bulk/Cakes as spawn


Extras: Filter Print Post Top
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 1 day
Re: AGAR ENVY! (Anything and All things agar!) [Re: mushroomnate]
    #25703922 - 12/26/18 01:56 PM (5 years, 1 month ago)


Well... I guess I can't pour plates? I waited longer this time, but still a lot of humidity... How do ya'll know when it's cool enough to pour? I tried resting a kitchen thermometer against the jar this time, but that's not very accurate I guess :frown:

What you're not seeing here is my Rusty White spores taking off! Yes! Both plates seem to have success!

Also, my PE transfers are taking off! They seem to like their new plates and are thickening up their myc.

My golden oyster LC2agar is ugly! I think there was a ton of junk in that live culture. Because of the condensation it's hard to see if anything good is happening in there, but I do see a lot of mycelium in there... I'm going to let it keep going fo a while.

I also learned that I'm not as sterile as I would like to be. After flaming my scalpel and cooling it in the agar, I can see nasty shit growing off the carbon it left behind. I guess I'm learning why agar is important for getting clean spawn.



First transfer to grains from agar (PE) is going well, both jars inoculated and we have take off!


Extras: Filter Print Post Top
OfflineDuhrwood Stevens
Gauche Chemoorganoheterotroph
Male


Registered: 09/28/18
Posts: 410
Loc: Allupin Sideya
Last seen: 3 years, 1 month
Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25703958 - 12/26/18 02:11 PM (5 years, 1 month ago)



--------------------
Last seen 12/2/2020


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Duhrwood Stevens]
    #25703974 - 12/26/18 02:16 PM (5 years, 1 month ago)

I pour when I can hold the media bottle comfortably, bare handed... sometimes, the bottom has started to coagulate, and the last couple of dishes end up a little thick... stack them as high as I can in the SAB... usually only the top 2 plates of each stack have condensation, and very little, which clears up before I use them typically


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 1 day
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25704004 - 12/26/18 02:30 PM (5 years, 1 month ago)

Quote:

Duhrwood Stevens said:
Get a point&shoot IR thermo, they're cheap.



They are, damn!
Quote:

Caps McGee said:
I pour when I can hold the media bottle comfortably, bare handed... sometimes, the bottom has started to coagulate, and the last couple of dishes end up a little thick... stack them as high as I can in the SAB... usually only the top 2 plates of each stack have condensation, and very little, which clears up before I use them typically



So, I'm doing it by when I can hold it in my bare hand... I cook a lot... Maybe my hands are broken?


Extras: Filter Print Post Top
OfflineDuhrwood Stevens
Gauche Chemoorganoheterotroph
Male


Registered: 09/28/18
Posts: 410
Loc: Allupin Sideya
Last seen: 3 years, 1 month
Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25704046 - 12/26/18 02:54 PM (5 years, 1 month ago)

140f is cool enough to hold but still 20+ degrees too hot. Compare it to coffee, sippin' temp is different than chuggin' temp. If it feels like 'last chance' coffee it's perfect.
With a thermo I've found 120f is gonna steam up way more than 116 but you run out of time fast at 116. Thick agar is worse to thicken up while pouring than soft IMO. :goodluck:


--------------------
Last seen 12/2/2020


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Duhrwood Stevens] * 1
    #25704062 - 12/26/18 03:02 PM (5 years, 1 month ago)

I'm talking comfortably... for normal folks: shower temp lol


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
OfflineDabadoodledoo710
Dab Connoisseur
Male


Registered: 03/04/17
Posts: 1,140
Loc: Zone 5b Flag
Last seen: 21 days, 12 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25704086 - 12/26/18 03:15 PM (5 years, 1 month ago)

Quote:

Caps McGee said:
I pour when I can hold the media bottle comfortably, bare handed... sometimes, the bottom has started to coagulate, and the last couple of dishes end up a little thick... stack them as high as I can in the SAB... usually only the top 2 plates of each stack have condensation, and very little, which clears up before I use them typically




I do this exactly with the same results!:thumbup:


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: Dabadoodledoo710]
    #25704090 - 12/26/18 03:17 PM (5 years, 1 month ago)

RW clone, T3:



--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
OfflineMH5109
Stranger
 User Gallery

Registered: 09/27/17
Posts: 1,385
Loc: America Flag
Last seen: 2 years, 11 months
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25704110 - 12/26/18 03:26 PM (5 years, 1 month ago)

Looking good caps.


--------------------




Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: MH5109]
    #25704118 - 12/26/18 03:33 PM (5 years, 1 month ago)

All stays well, prep more oats in a few days... got 14 qts RW, 4 qts PE6, and 2 quarts Texas going already... got the bug bad after thanksgiving/not cultivating anything but plates for like 6 weeks


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


Extras: Filter Print Post Top
OfflineDabadoodledoo710
Dab Connoisseur
Male


Registered: 03/04/17
Posts: 1,140
Loc: Zone 5b Flag
Last seen: 21 days, 12 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: MH5109]
    #25704125 - 12/26/18 03:35 PM (5 years, 1 month ago)

Couple CRS. Anyone know what the circular lines are from? Still grows out fine, just curious.


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* buying agar - help sinisha 3,416 6 03/31/06 05:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,382 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,155 8 02/25/05 11:03 AM
by agar
* test tubes agar fungophiliac 3,134 8 06/19/20 09:41 PM
by gorilliam
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 4,587 12 03/30/20 10:37 AM
by Surf707
* Agar and the global economy Dem_Bones 1,650 4 03/01/06 09:08 AM
by Dem_Bones
* Agar question WormholeSurfer 1,482 3 10/29/05 07:10 PM
by agar
* Agar's Home Made Compost recipe for bulk substrates
( 1 2 3 4 all )
agar 20,874 75 02/12/21 05:50 AM
by Bart84

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
540,822 topic views. 11 members, 146 guests and 48 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.009 seconds on 13 queries.