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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: YoshiTrainer's guide to low-prep grains [Re: oatsnhoes] * 2
    #29075385 -



Here's an update to my first post about trying with unmodified lids.

I dropped in some agar that had mycelium from a pin that was growing on another container of agar a while ago.
Looks like the method worked out alright so far.
I don't see anything crazy on them at the moment, so I will be spawning these when I find the time.

Edited by oatsnhoes (01/02/25 08:02 PM)

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Re: YoshiTrainer's guide to low-prep grains [Re: oatsnhoes]
    #29075492 -

Great job with the unmoded lids Oatsnhoes and good luck with your subs!


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


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Re: YoshiTrainer's guide to low-prep grains [Re: YoshiTrainer] * 1
    #29093279 -

Are those lids flipped?

So, I've been doing 10 jars in my Presto 23qt, but I've also been having jars stall and stuff. I think that despite tightening the lids, water leaks when its sideways, leading to not fully hydrated grains that don't colonize properly.

Maybe the lid choice matters, I've been doing old school polyfill ones on the top layer, maybe I'll try my newer plastic lids that I'm not too into.

So, moral of the story, in case the sideways 3 jars are a bad idea, today I did 3 PF Cakes in these pp5 food containers. Three of them fit perfectly on the top level, alongside my utensils and even a lil jar for sterile water.

So, if the no-prep amounts of water required are not working, this is a way of maximizing space, and encouraging the testing of cultures. 2 Parts Field Capacity Coir/1 Part Flour, mix up, hydrate a bit more, then case with pure coir and PC.



--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: YoshiTrainer's guide to low-prep grains [Re: Huskies]
    #29094406 -

Thanks for the info Huskies. I've not run jars sideways but I'd definitely worry about water leaking.


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


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Re: YoshiTrainer's guide to low-prep grains [Re: YoshiTrainer] * 1
    #29094430 -

Yeah in retrospect, I often have jars that seem to stall, and it is probably due to the above. Will try once more just in case and report back if I have more success.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: YoshiTrainer's guide to no-prep grains [Re: 99.99] * 1
    #29132448 -

Years later and this post is still helpful to newcomers like me. Thank you!


--------------------
Midnight Dragon
Nocturnal Mycologist | Fungi Adventurist
"Cultivating nature's magic, one mushroom at a time, rooted in tradition, thriving in the shadows."

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Re: YoshiTrainer's guide to no-prep grains [Re: MidnightDragon] * 1
    #29132612 -

Thank you and good luck MidnightDragon!


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


Extras: Filter Print Post Top
Re: YoshiTrainer's guide to no-prep grains [Re: YoshiTrainer] * 1
    #29132621 -

I think I found something that was causing me issues, contamination after spawning specifically.

I've been mostly using no prep millet. When spawning, I started checking under the lid before shaking the jar. Guess what?

At least with no prep millet, most of the time there would be a few uncolonized grains on the sides or directly under the lid. I started swiping down the inner part of the jars before shaking, removing anything that is suspect.

Seems to have been my main contamination vector. Or am I wrong? Wouldn't a half dozen uncolonized grains spell disaster?

anyone else got experience with this?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: YoshiTrainer's guide to no-prep grains [Re: Huskies]
    #29133634 -

Huskies said:
I think I found something that was causing me issues, contamination after spawning specifically.

I've been mostly using no prep millet. When spawning, I started checking under the lid before shaking the jar. Guess what?

At least with no prep millet, most of the time there would be a few uncolonized grains on the sides or directly under the lid. I started swiping down the inner part of the jars before shaking, removing anything that is suspect.

Seems to have been my main contamination vector. Or am I wrong? Wouldn't a half dozen uncolonized grains spell disaster?

anyone else got experience with this?


I suppose half a dozen uncolonized grains could be a vector when spawned in the open air. One way to reduce this would flicking the tops of your lids, after shaking, to knock down any grains that stick. I have to do this w/agar sometimes when it sticks to the lid. Another possibility would be to gently thump your jars on a thick rubber mat trying knock the grains down. Also, after your 30% shake, knock the grains down again.

"I started swiping down the inner part of the jars before shaking, removing anything that is suspect." I'm not sure how you're going about this but this sounds like another possible contam vector.


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


Extras: Filter Print Post Top
Re: YoshiTrainer's guide to no-prep grains [Re: YoshiTrainer]
    #29133644 -

I meant before breaking up the grains right before spawning, not during colonization. With individual clean paper towels. Figured that since its about to go to coir in open air it shouldnt matter.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: YoshiTrainer's guide to no-prep grains [Re: Huskies] * 1
    #29133695 -

Ok, I understand better now. I'd still recommend knocking any grains down at inoculation and after initial shake so hopefully at spawn time you won't have any uncolonized grains to worry about.


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


Extras: Filter Print Post Top
Re: YoshiTrainer's guide to no-prep grains [Re: YoshiTrainer] * 1
    #29134372 -

I will definitely try the lid tapping!


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Extras: Filter Print Post Top
Re: YoshiTrainer's guide to no-prep grains [Re: Huskies]
    #29140196 -

Anyone have the weight measurements for water and oats in pint jars? Does the general recommendation of 2/3C grains to 80ml water for pints need to be adjusted for oats? Thanks!

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Re: YoshiTrainer's guide to no-prep grains [Re: rcp] * 1
    #29140311 -

I think quarts are 200g oats:175g water so, 100g oats and 87.5g h20 for pints.


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


Extras: Filter Print Post Top
Re: YoshiTrainer's guide to low-prep grains [Re: MojaveMyc]
    #29329145 -

hi, water?

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Re: YoshiTrainer's guide to low-prep grains [Re: YoshiTrainer] * 1
    #29333177 -

I can’t understand why my bags (purchased from China, size 25×14×50 cm, 0.2 micron filter) keep bursting. I seal them before sterilization and autoclave them in a professional unit at a friend’s laboratory at 124 °C for 3 hours. When the cycle finishes, I don’t touch anything, I don’t vent, and I let the pressure drop on its own. I only open it after 24 hours. On top of the basket, I place a lid with two weights. Out of 10 bags, 5 burst.

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Re: YoshiTrainer's guide to low-prep grains [Re: gianniyak] * 1
    #29333661 -

Thank you for trying this out Gianniyak! My guess, assuming the bags are not the problem, is you are rolling the bags too tight? After adding the dry grain and water to your bags, loosely roll them or accordian fold the tops of the bags. With low prep, the grains expand in the bags, especially larger ones like oats, barley and wheat.

I've had some bags come out looking like pears but not split.

Good luck and please let me know if this helps.

Edit:
Just reread your post, sealing them before PC is most likely your problem? Try loosely rolling or accordian fold the tops. Seal them after inoculation or at least when you remove them from your cooled PC.


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


Edited by YoshiTrainer (09/03/25 10:47 AM)

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Re: YoshiTrainer's guide to low-prep grains [Re: YoshiTrainer] * 2
    #29333669 -

ive never done no prep bags but had similar issues with bursting when presealing back in the day.  i literally just fold my bags over now and they vacuum themselves closed pretty dam well during the pc cycle. as u can see i dont even put anything on top. ive tried it all and the most simplest way turned out to work best for my situation. no need to preseal really.


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Re: YoshiTrainer's guide to low-prep grains [Re: sloug] * 1
    #29335434 -

Thanks for the answer, Yoshi.

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Re: YoshiTrainer's guide to low-prep grains [Re: sloug]
    #29335440 -

Hi Sloug, do you think that, if I don’t seal them, I can let the PC vent right after finishing my 3 hours of sterilization, or will they still explode anyway?

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Shop: North Spore Cultivation Supplies, Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck, Certified Organic All-In-One Grow Bags by Magic Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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