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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Coir, Dessicant, Malt Extract, Pressure Cooker, Vermiculite

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Offlineerags
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Registered: 02/15/05
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Start to Finish - Complete Guide to Bulk Cultivation
    #3781652 - 02/15/05 02:06 AM (11 years, 9 months ago)

Start-To-Finish Bulk Mushroom Cultivation

  This technique can be used to produce high yields of mushrooms for study or consumption(edible species only). The process below is a concise combination of several well known techniques(many thanks to those original authors) and represents just one path you can take to get from spores to dried mushrooms. You are encouraged to read the FAQs posted elsewhere to fully understand the techniques found in this outline.  There is no substitute for learning first-hand from the process, but if you've got your head on straight or have any experience following protocols (laboratory or science coursework), you should be able to have a successful low hassle grow; best of luck! :goodluck:

  :alert:The time course given is approximate and depends on individual conditions. Mycellium should always appear a clean white; discard specific substrate if discoloration appears or contamination is even remotely suspected. Preparing several separate liquid, grain or bulk preparations simultaneously is suggested to allow you to discard suspect substrates while continuing the process.

Phase 1:  Spore Syringe
:syringe: (1 day)

Materials:

Small Sterile Syringe 10 cc
Small glass vessel (Double shot glass)
Metal cooking tray
Spore print
Lighter
Isopropyl rubbing alcohol
Paper wipes

Procedure

1. Clean hands and workspace thoroughly, using rubbing alcohol and wipes.
2. Microwave water in micro-safe bowl or jar for 8 min to sterilize.
3. Suck 10 cc of sterilized water into syringe and allow to cool.
4. Put a metal tray in a clean/sterilized oven with a glass vessel in the middle of the tray. Heat to 400?F for 30 min. Let oven and contents cool completely.
5. Place oven on warm briefly (190?F), to allow air flow upward and away from materials.
6. Open oven door and pull out rack with tray and vessel.
7. Wipe syringe tip with rubbing alcohol and sterilize with flame.
8. Squirt 4 cc of sterile water into glass vessel.
9. Scrape small quantity of spores into vessel with water. 1/5th of a print is adequate.
10. Using syringe and remaining sterile water, mix spores and water with gentle suction, in and out 5 times.
11. Suck all liquid back into syringe, sterilize tip, recap and store in sterile container for 12 hrs prior to inoculation.



Phase 2: Liquid Culture (~8 days)

Materials:

Distilled water
Hydrogen Peroxide: 3% H2O2
Malt extract powder; found at home brew store, use lightest colored malt
Quart canning jars: Two small punctured holes in lid
Tyvek (paper-like packaging material found at post office); place under lid
Pressure cooker
Spore syringe
Incubation chamber: 2 large storage bins, 5 gal fish tank adjustable water heater.


Procedure:
1. Make incubation chamber by stacking two storage bins; place water in bottom bin along with fish tank heater. Adjust to 86? F. Do in advance a couple days to make sure temp is constant.
2. Dilute malt extract in hot distilled water; 1 Tblsp / cup water (4% by weight).
3. Pour malt extract solution into jars. Place Tyvek under perforated lid.
4. Place in pressure cooker at 15 psi for 20 min.
5. Let cool over night.
6. Sterilize spore syringe needle with alcohol and flame.
7. Inject 1-2 cc from spore syringe through Tyveked lid. Swish jar gently.
8. Place jar in incubation chamber at 86? F. Agitate jars daily.
9. After 2 days inject 3-4 cc of 3% H2O2 . This is an extra anti-contam precaution.
10. When milky consistency and floating mycelium (looks like jellyfish) is observed move jar to refrigerator for storage. This is the liquid culture.

Phase 3: Grain Spawn
(~12 days)

Materials:

Popcorn
Quart jars; perforate 2 small holes in lid
Tyvek (paper-like packaging material)
Strainer
Large syringe, detachable large metal needle
Pressure cooker
Incubation chamber: 2 large storage bins, 5 gal fish tank adjustable water heater
Distilled water

Procedure:

1. Measure out enough popcorn grain to fill all needed jars 2/3 full.
2. Wash the popcorn grain thoroughly 3 times.
3. Soak the grain 24 hours in water; ? inch water over the top of grain.
4. Simmer grain gently on stove in pot for 1 hour.
5. Drain thoroughly in strainer for 30 min.
6. Pack quart jars 2/3 full of strained grain. Seal with perforated lid over Tyvek.
7. Cook grain jars and large syringe needle (no plastic parts) in pressure cooker at 15 psi for 70 min.
8. After cooling completely shake the jar to loosen grain.
9.Sterilize large syringe tip with alcohol and flame.
10. Shake Liquid Culture vigorously and inject 3-4 cc into grain jars swirling liquid around the sides of the jar.
11. Place jars in incubation chamber till completely colonized, shake every 4-5 days as necessary. This is your grain spawn.

Phase 5: Bulk substrate (~10 DAYS)
Materials:

Horse/Cow dung: aged, dried
Wheat Straw
Plastic oven bags
Oven safe thermometer (external display and temp alert suggested)
Grain spawn
Lime (gardening Supply)
Tin Bread pans



Procedure:

1. Hydrate straw/dung in oven bags mixture to ?field consistency? (a little more wet than damp). When squeezing moistened substrate in hands, a little trickle of water should come out.
2. Tie off the oven bag with string, thermometer inserted through the tied opening.
3. Place bag in oven heated to 300?F.
4. Watch thermometer often during first hour. Keep temperature below 185?F. When thermometer read 170? ? 180? F turn oven temp down to 190?-200?.
5. After a total 3 hr cooking time remove bag from oven.
6. Remove thermometer and retie opening to seal it.
7. Let cool 6- 10 hrs.
8. Mix grain spawn, cooled bulk substrate, and a dash of lime in sterile bag or container, using sterile gloves and work area. This is your bulk substrate.
9. Add an even layer of bulk substrate into pan to about 1 inch from top.
10. Place black plastic tightly over surface of bulk substrate.
11. After a week check for complete colonization of mycelium. Then case.

Phase 5: Casing (~5) days

Materials:

Lime (gardening Supply)
coco coir (i.e. Bed-A-Beast found at pet stores)
Vermiculite (gardening Supply)
Bulk substrate

Procedure:

1. Hydrate coco coir in warm water completely.
2. Mix to 60/40 ratio by volume of Vermiculite to coco coir.
3. Add lime, 5% by volume.
4. Heat mixture in sterilized microwave for 5 min and allow to cool completely.
5. Ring out excess water to reach field consistency for this casing mixture.
6. Coat bottom of pan with thin layer of vermiculite as drainage layer.
7. Add even casing mixture as top layer; approx 2/3 - 3/4 in.
8. Place a perforated tin foil or black plastic lid loosely on top; this is your cased pan.



Phase 6: Cultivation (~6 days)

Materials:

Spray bottle, fill with water and 3% H2O2, 10% by volume.

Procedure:

1. Place cased bread pans in dark, room temperature area.
2. Check daily. As mycellium appears on the top of the casing add thin layer casing mix to these white areas until even white distribution is observed.
3. To initiate pinning remove foil/plastic lid twice daily and/or slightly reduce temperature.
4. When pins appear, lightly mist with spray bottle and expose to low light for a few minutes daily.
5. In an arid environment, it may be cover pans with black plastic (garbage bags) to hold in moisture, throughout cultivation.


Phase 7: Harvest and storage ( ~3 days)


Materials:

Desiccant, found at paint store
Wire mesh, or rack
Storage bin
Fan

Procedure:

1. With twisting motion pull almost mature mushrooms from casing surface. Pick when the veil is almost pulling away from the stem but mostly attached.
2. Continue to mist-hydrate cased pans generously with spray daily for further flushes.
3. Air dry mushrooms on rack with a fan until ?cracker? dry.
4. Place dried mushrooms in storage bin on rack above a layer of desiccant.
5. Dry over night.
6. Package in air tight container and store at reduced temperature to increase life.
How useful is it to have a complete tek?
Users may choose only one (23 total votes)
not very, indiv teks suffice
-
3 13%
somewhat
-
1 04%
no opinion
-
3 13%
useful
-
10 43%
essential
-
6 26%
Votes accepted from (02/15/05 02:00 PM) to (02/15/06 02:01 PM)
You must vote before you can view the results of this poll.


Edited by erags (02/15/05 09:09 PM)


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OfflineAnnoA
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Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: erags]
    #3781700 - 02/15/05 02:19 AM (11 years, 9 months ago)

>How useful is it to have a complete tek?

Well, it is a nice idea, the problem is that with this short description, you have to already know and master all the steps in order to replicate it. For a newbie the descriptions are much too short and unspecific.


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InvisibleNeedMoreSleep
OCD = 100%Sterility :-)

Registered: 01/25/05
Posts: 1,210
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Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: Anno]
    #3781715 - 02/15/05 02:23 AM (11 years, 9 months ago)

Quote:

Anno said:
For a newbie the desciptions are much too short and unspecific.




Agree :thumbup: ... but with a little elaboration and maybe some pics, would be nice... :stoned:


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
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Invisibleshobimono
Why?
Registered: 09/14/04
Posts: 560
Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: Anno]
    #3781749 - 02/15/05 02:35 AM (11 years, 9 months ago)

pictures, pictures, pictures
Grow guides without pictures are dry and boring, and as anno mentioned, without thorough descriptions, they aren't very helpful for a true newbie.
For myself, I would learn more reading cultivation forums (cuz a lot of times people include pics with their posts), then I would reading a text with no pics grow guide.


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OfflineLaughingJim
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Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: shobimono]
    #3782004 - 02/15/05 03:57 AM (11 years, 9 months ago)

"A dash of lime..." ???

Is lemon a valid replacement? :smile:


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OfflineIamthewalrus
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Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: erags]
    #3782082 - 02/15/05 04:51 AM (11 years, 9 months ago)

there is no tek thats gonna magically make ppl understand how to grow...good flushes come from experience

there are just way to many lil things like anno mentioned that have to be learned...I've said it before and I'll say it again...anyone can grow a cube...but to grow constient sweet flushes takes skill and experience


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Invisibleagar
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Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: Iamthewalrus]
    #3782194 - 02/15/05 05:47 AM (11 years, 9 months ago)

>>>>to grow constient sweet flushes takes skill and experience<<<<

AGREE :thumbup:, ........PATIENCE & PERSISTANCE.......also :thumbup:


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OfflineVertigo6911
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Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: Iamthewalrus]
    #3782212 - 02/15/05 06:05 AM (11 years, 9 months ago)

i think a complete guide can be very usefull for a noob.
i started this january and ive read several of them and they were
very helpfull in getting an overview of the process.
i ended up compromising elements from several of these guides,
together with a bunch of faq stuff ending up with a greatyly simplified version...

so there is a use for it aside from following it to the letter...
every tek should have a notice saying: "read the faq first".
also in my experiance so far, the availability of products and equiptment, or lack thereof forces alot of peaple to compromise.
therefore i think its always good to have well difined lines between the different processes so one can frankenstijn them to fit their needs.


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Offlinemol666
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Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: Vertigo6911]
    #3782374 - 02/15/05 08:28 AM (11 years, 9 months ago)

Thats one fine guide..
Its going to help a foaf..
Pics would be Great...


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Offlineerags
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Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: erags]
    #3785184 - 02/15/05 08:59 PM (11 years, 9 months ago)

Thanks for the feed back , I agree its good to treat this as an overview. It can be overwhelming for a first timer to visualize all the steps described in FAQs together.

I observed this particular process carried out at a remote location with minimal effort, using Ecuadorian. I felt it should be recorded to help others considering approaches. Call it paranoia, but I didnt take any photos, no dig camera either at that time, but I have one now.

"Someone" injested close to 4 grams of dried little aborts 3 days after harvest. Seeing intense visuals 15 minutes afterward and being plunged into ego-less chaos soon after, he decided not to do mushies for a while. There is such a thing as too much. I think real horse shit helps.


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Edited by erags (02/15/05 10:47 PM)


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OfflineRiggidy
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Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: erags]
    #3970910 - 03/26/05 01:13 AM (11 years, 8 months ago)

This was not ment as a complete guide, follow the simple directions along with the FAQ and even the complete noob can have succese.


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OfflineRiggidy
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Re: Start to Finish - Complete Guide to Bulk Cultivation [Re: Riggidy]
    #3970919 - 03/26/05 01:15 AM (11 years, 8 months ago)

I helped make up the steps as a first timer which also shows the two first timers at seperate time can have no problems at a "larger grow." Which goes to show "Fuck the PF teck" just case.


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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Coir, Dessicant, Malt Extract, Pressure Cooker, Vermiculite

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