Rotnpins said:

I was thinking the same thing about the color and disorganization, but I wasn't sure so I didn't say anything... but that whole group of plates you posted has a funny look to them.. maybe a TC can take a look at the pictures for you

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What is a TC?

Uploading pics now!

Thanks,

Rusty2096 said:

I am not experienced enough to look at more pics (was just giving you my humble opinion).

But you sure should post them so someone with more xp can look at em

(TC stands for Trusted Cultivator)

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Here is more pics, thanks for your honest answers!

gabbk said:

Hey shroomsurf!
To me it also looks kinda sketchy. I'd definitely keep transfering if I were you. Maybe changing to another recipe could homogenize everything up a little bit. Or even better if you could also transfer from other plates. Just remember transfering from the leading edge and keep it small, with the size of a grain of rice or so. Either way, if you decide to transfer it to grain, there you will notice if the myc was healthy or not.
By the way, TC means 'trusted cultivator'. It's a label you see under the usernames of those who are activelly contributing to the forum

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Thanks, I'll either do another T or just do another strain from MSS onto agar tonight...

I haven't done my own agar yet, but maybe that's the problem so I think I'll use another genetics to confirm about the agar...

Thank you...

shroomsurf said:

Rusty2096 said:

I am not experienced enough to look at more pics (was just giving you my humble opinion).

But you sure should post them so someone with more xp can look at em

(TC stands for Trusted Cultivator)

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Here is more pics, thanks for your honest answers!

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Rusty2096 said:

I'm sorry I was the original party pooper. I legit looked at your pic for the longest time and it took me hours to decide to post about it (because of uncertainty and not wanting to break your positive spirit you had going there).

Whatever happens, healthy myc or not, don't wave that flag --->

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I like knowing when something sucks, that way I'll not waste my time and move forward towards something that doesn't suck...

Already have my Jedi, print scraper and 5 cups ready for tonight...



Thanks for your observations...

Smellyhobbit said:

No one listen to me when I say your plates are good lmao. I think I’ll just abstain from doing so for a few months.

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Agar Wars...

Smellyhobbit said:

No one listen to me when I say your plates are good lmao. I think I’ll just abstain from doing so for a few months.

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Rusty2096 said:

Smellyhobbit said:

No one listen to me when I say your plates are good lmao. I think I’ll just abstain from doing so for a few months.

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“He that is without sin among you, let him first cast a stone at her.”

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This post has been here forever, guess nobody is messing with Jesus...

I had mask on, gloves, in front of the hood, heat flamed scraper couple times, 70% stuff and went from close to the hood towards me...

And I just put spores onto 9 plates to beat the odds...

Might have put way too much spores, but this is how I learn by growing mold and over spore'ing ...lol

"Never tell me the odd's"

shroomsurf said:

Rusty2096 said:

I'm sorry I was the original party pooper. I legit looked at your pic for the longest time and it took me hours to decide to post about it (because of uncertainty and not wanting to break your positive spirit you had going there).

Whatever happens, healthy myc or not, don't wave that flag --->

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I like knowing when something sucks, that way I'll not waste my time and move forward towards something that doesn't suck...

Already have my Jedi, print scraper and 5 cups ready for tonight...



Thanks for your observations...

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LtLurker said:

You didn't goof by flaming then using iso on the blade did ya? Would explain this quick and ez fix.

I don't use a fh but the direction sounds backwards. Shouldn't you bring in tools from your body towards the plate and hood? Since it blows towards you, any tams you accidently bring in will blow from the tool towards you and hit anything in between. Maybe it's counter-intuitive though. Another fh user might correct me on this one

Another thing to check i can think of is the laminar flow. If the speed is off that would cause a full set to fail trying to work in turbulent air.

Trying to think of other things that could cause total failure. Dirty spores is possible, but probably wanna check other stuff before blaming them.

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Hi, I learned from someone's to not iso after flame so I got that one ...

I also heard from someone to go from hood to me when doing FH work but I'm also curious others methods!? Maybe the thought of having to go back to one's self after being at the filter is over risking contam, instead of from the hood once to one's self, not sure that's intuitively correct, but it's less moves (from the hood to oneself) logistically speaking (uses fancy agar words...lol)

Yea, I looked again and I put way to many spores, but I figure I'll have to do atleast T1's and I have 9 on board to choose from!

I did the smoke test on the FH as did I talk in person to the designer and he said he designed really carefully with test equipment, but with my "test incense equipment" the smoke comes out real steady to about 18" past the filter and in a 13" X 15" heighth and width. I would have been upset if it hadn't and called the guy again but it looks like clean air in kinda a pretty big space to do laminar work...

Here is the FH I purchased...

https://mycology-supply.com/collections/air-filter-units/products/mycology-supply-laminar-flow-hepa-filter-13-x-15

It was either that or the labrat 13" X 15" FH, both have the same filter/pre-filters, just mine is less deep but same heighth/width, plus mine is more portable and way lighter...

Thanks for your helpfulness...

wild_aussie said:

Hey I can recognize my handwriting there lol glad you are get those guys to spores they were a faster colonizer/fruiter than some of the other cubes I've run. At least in my experience

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Thank you wild_aussie, hope Jedi is nice to work with and hopefully fruit nicely...

p9hu7 said:

Move the exposed culture over objects to remain in flow, never behind objects where turbulence could deposit contaminants on your wedge.

Keep your hands to the rear of any object in flow while inoculating, open lids without placing hands between the jar and the filter.

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