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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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mutant screening
    #6795165 -

what about screening fungal lines for enhanced production of tryptamines, after exposing the spores with uv radiation, a high majority would be sterilized but you could find some interesting results.

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Re: mutant screening [Re: mrmackey]
    #6795176 -

How?
RR


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Re: mutant screening [Re: RogerRabbit]
    #6795229 -

In the 70's people used colchicine from the winter crocus as a potency booster with marijuana crops, the problem is, its poisonous meaning you wait until the sencond generation to screen the mutants, I thought using a uv light, like the kind they use in food steralization units and flow hoods , would work in low doses as a mutagen, of course it means screening stocks made with isolated spores,

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Re: mutant screening [Re: mrmackey]
    #6795232 -

You will first need a way to accurately quantify the levels of tryptamines in the dried mushrooms to be able to determine differences between fruiting cultures. Asking friends for potency ratings after eating them isn't good enough.


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Re: mutant screening [Re: Workman]
    #6795255 -

ether extraction of psilocybin from liquid cultures, measure the yield weight of standardized extracts

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Re: mutant screening [Re: mrmackey]
    #6797030 -

You could use a DNA cross-linker, like a strata-linker to expose the spores to UV. More likely you would be best to just use a germicidal UV lamp. You could figure out the exposure required to get 1% germination and you would end up with a good percentage of mutants.

That's a pretty standard method at least. You never know what you might find.


-FF

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Re: mutant screening [Re: Workman]
    #6806375 -

Subjective potency ratings with a double-blind *would* be good enough, if you did it to some scale.

But uh, I don't guess anyone has a big ol' focus group lying around.


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Re: mutant screening [Re: figgusfiddus]
    #6806496 -

some people with serious experience should start with simple mutagens uv, or something like ethidium bromide, if you do it with plants you often get huge results from viable lines. What you guys want is an initiative where people are experimenting and sharing data and culture samples.

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Re: mutant screening [Re: mrmackey]
    #6806579 -

I think this would be too cumbersome a task without a reliable method for measurement. What you're describing is a full-time job--actually, twelve or twenty full-time jobs. Tack on the fact that testing through trial takes five hours per go, and you've got a big fat impractical mess.

Now, if one of you folks has a mass spectrometer... heh.

I think substrate additives have a better chance of improving potency in the long run than artificial selection. You've got a practical cap in terms of protein content, and it's no wonder that cubes--considering their fave substrate and size--have a rather standard ceiling in terms of psilo content.


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Edited by figgusfiddus (04/18/07 10:14 PM)

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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