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Invisibletheshiftingwalls
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Registered: 06/18/03
Posts: 4,128
Loc: Residing in thee Universa...
Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: poke smot!]
    #1899849 - 09/09/03 07:32 PM (20 years, 4 months ago)

Lets say this E enzyme has a limit of a gram.


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Invisibletheshiftingwalls
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Registered: 06/18/03
Posts: 4,128
Loc: Residing in thee Universa...
Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: theshiftingwalls]
    #1903636 - 09/10/03 11:43 PM (20 years, 4 months ago)

4-HO-DPT will form in the mushrooms fruit bodies just like Baeocystin forms also.


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Invisiblepoke smot!
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. *DELETED* [Re: theshiftingwalls]
    #1904414 - 09/11/03 06:28 AM (20 years, 4 months ago)

Post deleted by poke smot!

Reason for deletion: x



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Invisibletheshiftingwalls
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Registered: 06/18/03
Posts: 4,128
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: poke smot!]
    #1904971 - 09/11/03 11:04 AM (20 years, 4 months ago)


I have no idea really... But lots of people need to test with DPT.


Edited by theshiftingwalls (09/11/03 12:07 PM)


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Invisiblepoke smot!
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. *DELETED* [Re: theshiftingwalls]
    #1905071 - 09/11/03 11:35 AM (20 years, 4 months ago)

Post deleted by poke smot!

Reason for deletion: x



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Invisibletheshiftingwalls
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Posts: 4,128
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: poke smot!]
    #1905081 - 09/11/03 11:38 AM (20 years, 4 months ago)

Hush hush...


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Invisiblepoke smot!
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. *DELETED* [Re: poke smot!]
    #1905112 - 09/11/03 11:50 AM (20 years, 4 months ago)

Post deleted by poke smot!

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Invisibletheshiftingwalls
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Registered: 06/18/03
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: poke smot!]
    #1905142 - 09/11/03 12:06 PM (20 years, 4 months ago)

But 4-HO-DPT effects are like psilocin. 4-HO tones down the effect and brings waves :wink:. 4-HO-DPT is very very different from DPT. (eg DMT to 4-HO-DMT psilocin)

Plus, I do not think any DPT will be left over in the mushrooms. So only 4-HO-DPT will be left.

The experts just need to figure out all the hard things.


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Invisibletheshiftingwalls
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: theshiftingwalls]
    #1916424 - 09/14/03 07:33 PM (20 years, 4 months ago)

Anyone test this idea yet? (bump)


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Invisiblemycofile
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: theshiftingwalls]
    #1917687 - 09/15/03 08:55 AM (20 years, 4 months ago)

Quote:

The experts just need to figure out all the hard things.



There are no experts, that's the problem. Of the maybe 5 "experts", I bet all of them have many more things that they are personally more interested in than this.

We do have dedicated amateurs, but this is beyond the scope of all but maybe a couple.

1. Creating new drugs, in new ways, and testing them only via bio-assay is a good way to die, thus

2. Quantitative testing of controlled substances is illegal to the general public in the US

3. Person performing experiments would have to be non-US

4. Person perfoming experiments would need money to do have quantitative tests performed to work out yield and dosage.

I mean look how long it's taken for somebody to do a quantitative study of potency from various substrates. Anno has taken that under his wing, but it's still not done, and it's not even as scientifically rigorous as what you suggest.

In fact, Anno is about the only person I could see actually doing any of this. At that, he would need the funds to aquire the dpt, the time and effort to set up a decently scientific experiment (I'm sure that wouldn't be a problem), and the funds to have the resulting fruits tested from each experimental batch, and over several flushes to determine consistency of yield. That's a lot to ask of someone, but perhaps it may happen.....one day.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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OfflineAlbertHoffmann
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Registered: 09/15/03
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: mycofile]
    #1917887 - 09/15/03 10:59 AM (20 years, 4 months ago)

What about using Mimosa Hostillis rootbark in your substrate? That stuff is LOADED w/ DMT, and you can buy it at a number of places on the web. Would that make the shroomies stronger? I may have to try it.


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OfflineInfrared
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: AlbertHoffmann]
    #1919162 - 09/15/03 06:25 PM (20 years, 4 months ago)

using tryptamine alone increases potency supposedly, i would just save your n.n dmt for smoking. this is rather interesting thread though shifting even though this is not a new idea. i think i would rather supplement my substrate with tryptamine than dpt. although 4ho dpt sounds interesting i think i would rather have ultra potent 4hodmt shrooms. ill just use my dpt for dosing not supplementation.


--------------------
When chemistry is outlawed.. Only outlaws have chemistry:rainbowdrink:


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Invisibletheshiftingwalls
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: Infrared]
    #1919207 - 09/15/03 06:38 PM (20 years, 4 months ago)

Very cool :thumbup:.


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Invisibledoktor_alternate
card-carryingAmateurMycologistsAnonymous member
Registered: 08/24/03
Posts: 119
Loc: vancouver
Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: Infrared]
    #1919325 - 09/15/03 07:02 PM (20 years, 4 months ago)

hmmmmn. it seems that chemistry is a bit much for us simple farmers.
i say this: go to www.the-hive.ws
in the forum under "tryptamine chemistry" there is loads of information on the biosynthetic pathways of psilocin et al. prodution.
i have blown my mind to bits on 100mg DPT insufflated. why go to a reduced potency by 4-hydroxylating it?

https://www.the-hive.ws/forum/showflat.p...part=1&vc=1
(or, forum "enhanced pcilocybin production")

there is loads and loads of stuff there to answer your questions.


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Invisiblemicro
bunbun has a gungun
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: doktor_alternate]
    #1919920 - 09/15/03 09:56 PM (20 years, 4 months ago)

I think that the most probable scenario would be brought fourth by using DMT as a substrate. This is the starting point. If something else works that's more complicated, so be it, but if DPT works, DMT will work better.

Like I said before, the limiting factor will be downregulation (on a certain enzyme) and also the enzymatic degredation of the sidechain precluding the possibility of even steric compounds produced on that pathway.

It's not going to be very efficient; I'll tell you that. If you were to try and maximize the psilocin content, however, that's a different story. I really believe growing mushrooms off of Phalaris grass will increase their potency by a noticable factor. When you add another compound, however, with a really big sidechain, you're jumping ahead of the gun. The orgainsm will have downregulation mechanisms which will apply to even the metabolites of the given precursor -- this is where equilibrium is established. Thus, the question of how pragmatic the biotransformation will be comes into play. Since it can't be near 1:1 it will not be that pragmatic, chemically. The other limiting factor, degredation, cannot be tested outside of a lab.

If anyone has any *conclusive* results, I will be happy to help with the quantitative data. These data can be based on subjective results, as long as they are conclusive.

-- Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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Invisibletheshiftingwalls
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Posts: 4,128
Loc: Residing in thee Universa...
Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: micro]
    #1920055 - 09/15/03 10:37 PM (20 years, 4 months ago)

Using pure DMT is a waste. DPT is far much less costly. Im sure it works.

Trying a rare 4-HO-DPT with high content of 4-HO-DMT bio product would be a whole new type of trip.

The DPT (tryptamine) could would make more 4-HO-DMT alone. Also making a new product (4-HO-DPT) not of nature.


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OfflineInfrared
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: theshiftingwalls]
    #1921610 - 09/16/03 01:08 PM (20 years, 4 months ago)

yea using dmt would be a waste, if you want more 4hodmt production just use tryptamine which would be far less expensive and time consuming as using dmt would be. my next batch im definately gonna use tryptamine additive, ill do a log when i recieve my spores.


--------------------
When chemistry is outlawed.. Only outlaws have chemistry:rainbowdrink:


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Invisiblemicro
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: Infrared]
    #1923538 - 09/16/03 10:28 PM (20 years, 4 months ago)

Expensive?!?!

Grow some Phalaris grass, dry it, and use it as a bulk substrate. I was going to try this, but J** screwed me out of my order and I've been too busy to get back to them....

I'm not sure about the oral dosage of 4-HO-DPT, but if it's much more than a hundred mg I'd say you're wasting your time.

If it's really potent you may be onto something, but still, it has to be tested.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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Invisibletheshiftingwalls
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Registered: 06/18/03
Posts: 4,128
Loc: Residing in thee Universa...
Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: micro]
    #1923602 - 09/16/03 10:45 PM (20 years, 4 months ago)

Micro 4-HO-DPT has only been tested in the 20 mg with threshold results. Meaning that some thing wonderful was around the coner with a higher dosage.

4-HO-DPT starts to go beyond threshold effects at 40 mg.
So 100 mg of 4-HO-DPT would be a nice level 2 trip :smile:.

Phalaris grass sounds like a good idea as a bulk substrate.




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Invisiblemicro
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Re: Growing high content 4-HO-DPT/4-HO-DMT mushrooms. [Re: theshiftingwalls]
    #1923663 - 09/16/03 11:04 PM (20 years, 4 months ago)

If it's active at ~ 20 mg you *might possibly* be onto something! Of course, it needs to be tested, but since psilocin is active at ~ 50 mg (I'm guessing) it's impossible to say what the addition of DPT will do. This is a really interesting article!

The only problem is who knows how it will be broken down? Fungi has a lot of enzymes so these things are only known through testing. No matter what, however, by experimenting, you're learning things. I've been really busy, but if some conclusive data is present, I'll be happy to help with quantitative results (still as an anonymous person, of course.)

Only through the promotion of science will technology evolve. Since people certainly aren't evolving, what else is there?

Thanks for the insight,

--
Micro

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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