DERRAYLD said:

aNewBeginning said:

With petri dish lids, what is good sterile technique when working in an SAB?

My process looks like this

• Flame scalpel
  
• Lift lid of donor petri dish + hold lid above dish with left hand
  
• Cut a wedge with right hand
  
• Place lid back on petri
  
• Flame scalpel
  
• Lift lid of donor petri dish + hold lid above dish with left hand
  
• Stab the wedge with right hand
  
• Place lid back on petri
  
• Lift lid of receiving petri dish + hold lid above dish with left hand
  
• Place wedge on receiving dish with right hand
  
• Place lid back on petri
  

The process is somewhat frustrating because it is not easy to cut a wedge using one hand while holding the petri lid with the other hand.

Would it be risky to place the lid down somewhere in the SAB and hold the dish with one and and cut a wedge with the other hand?

Where could I place the lid and should it be face up or face down?

---

Use the petri lid to hold the petri while cutting, replace the lid once cut and move the transfer to the new dish using the lid from the new dish to hold down the receiving dish again.
Only open the dish enough to pass the transfer through.

---

aNewBeginning said:

DERRAYLD said:

aNewBeginning said:

With petri dish lids, what is good sterile technique when working in an SAB?

My process looks like this

• Flame scalpel
  
• Lift lid of donor petri dish + hold lid above dish with left hand
  
• Cut a wedge with right hand
  
• Place lid back on petri
  
• Flame scalpel
  
• Lift lid of donor petri dish + hold lid above dish with left hand
  
• Stab the wedge with right hand
  
• Place lid back on petri
  
• Lift lid of receiving petri dish + hold lid above dish with left hand
  
• Place wedge on receiving dish with right hand
  
• Place lid back on petri
  

The process is somewhat frustrating because it is not easy to cut a wedge using one hand while holding the petri lid with the other hand.

Would it be risky to place the lid down somewhere in the SAB and hold the dish with one and and cut a wedge with the other hand?

Where could I place the lid and should it be face up or face down?

---

Use the petri lid to hold the petri while cutting, replace the lid once cut and move the transfer to the new dish using the lid from the new dish to hold down the receiving dish again.
Only open the dish enough to pass the transfer through.

---

Thanks for your response.

Do you mean place the petri dish into the petri lid (upside down) while cutting?

---

aNewBeginning said:

With petri dish lids, what is good sterile technique when working in an SAB?

My process looks like this

• Flame scalpel
  
• Lift lid of donor petri dish + hold lid above dish with left hand
  
• Cut a wedge with right hand
  
• Place lid back on petri
  
• Flame scalpel
  
• Lift lid of donor petri dish + hold lid above dish with left hand
  
• Stab the wedge with right hand
  
• Place lid back on petri
  
• Lift lid of receiving petri dish + hold lid above dish with left hand
  
• Place wedge on receiving dish with right hand
  
• Place lid back on petri
  

The process is somewhat frustrating because it is not easy to cut a wedge using one hand while holding the petri lid with the other hand.

Would it be risky to place the lid down somewhere in the SAB and hold the dish with one and and cut a wedge with the other hand?

Where could I place the lid and should it be face up or face down?

---

A.k.a said:

I used to set the lids face down on an iso soaked paper towel in the SAB.


It’s definitely better to learn how to hold the plate and lid in place but it’s not horrible to take it off for a second if your technique is solid.


I ended up getting a piece of plastic sheet and cutting a hole just big enough to slide a plate into so it couldn’t move. Made things way easier.

---

verum subsequentis said:

"It's astonishing, it works well!"
Been tryin to tell folks for years.
#GladI'mNotRetarded
RIP Lotkid

---

verum subsequentis said:

"It's astonishing, it works well!"
Been tryin to tell folks for years.
#GladI'mNotRetarded
RIP Lotkid

---

verum subsequentis said:

PS the GLAD I'm Not Retarded is an old joke between me and Lotkid.
That was not aimed at you.

---

Quirkmeister92 said:

Glad I made the switch to cling wrap. I got some hostess brand rolls from the bargain outlet and it's pretty good. I need a dedicated cutting tool that wont pinch the wrap causing it to stick at the edges when using.

Some say a pipe cutter is good. Not a fan of knifing.

---

I used a serrated knife then sliced the spiky bits off with a scalpel.

Haven't heard of a pipe cutter before, will look into that.



---

Ps. cyanescens.





Isolating it more and more just out of curiosity.
It seems to shift between looking like a monoculture and showing obvious sectors.



Ps. tampanensis.




Hoping for some stones on agar

aNewBeginning said:

Any other agar tips you've been trying to tell folks for years?

You have to understand, for a rookie, there is an overwhelming amount of information on this website to sift through.

---

Advanced search is here:
https://www.shroomery.org/forums/search.php

I limit it to searching only from the last 5 years. A lot before that isn't the best info anymore.

Also tick the box
"Only show one result per thread"
or you'll get too many results from the same thread.

If you get too many results, MAYBE only search for posts by Trusted Cultivators. Though there are solid non-TCs. You'll start to notice after a while. Often (but not always), people with good replies have a lot of General Reviews (click the shrooms above their avatar over on the left.)

If still to many results, maybe limit to last 3 years.
=--=-=
As for cult pix, this is all I got today, fungus fam:

nektar61 said:

aNewBeginning said:

Any other agar tips you've been trying to tell folks for years?

You have to understand, for a rookie, there is an overwhelming amount of information on this website to sift through.

---

Advanced search is here:
https://www.shroomery.org/forums/search.php

I limit it to searching only from the last 5 years. A lot before that isn't the best info anymore.

Also tick the box
"Only show one result per thread"
or you'll get too many results from the same thread.

If you get too many results, MAYBE only search for posts by Trusted Cultivators. Though there are solid non-TCs. You'll start to notice after a while. Often (but not always), people with good replies have a lot of General Reviews (click the shrooms above their avatar over on the left.)

If still to many results, maybe limit to last 3 years.
=--=-=
As for cult pix, this is all I got today, fungus fam:

---