rido said:

These MEA plates were inoculated from LTC's Pan cyan Rainbow Eucalyptus print almost a week ago. Swabbed a tiny spot on the print, then streaked three plates using the quadrant method without returning to the print. I'm looking to make a few transfers soon before the colonies all run together. Any suggestions, or is it too early to tell?

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This doesn't look good to me. The Rainbow Eucalyptus print I streaked was extremely
bacterial. I swabbed 3 plates and the first 2 were just nothing but bacterial
goo. The 3rd plate was better and I was able to make 3 transfers. They
are all looking like this pic here, which is exactly the type of myc that I
want.

Asura said:

rido said:

These MEA plates were inoculated from LTC's Pan cyan Rainbow Eucalyptus print almost a week ago. Swabbed a tiny spot on the print, then streaked three plates using the quadrant method without returning to the print. I'm looking to make a few transfers soon before the colonies all run together. Any suggestions, or is it too early to tell?

---

This doesn't look good to me. The Rainbow Eucalyptus print I streaked was extremely
bacterial. I swabbed 3 plates and the first 2 were just nothing but bacterial
goo. The 3rd plate was better and I was able to make 3 transfers. They
are all looking like this pic here, which is exactly the type of myc that I
want.

---

Asura said:

rido said:

These MEA plates were inoculated from LTC's Pan cyan Rainbow Eucalyptus print almost a week ago. Swabbed a tiny spot on the print, then streaked three plates using the quadrant method without returning to the print. I'm looking to make a few transfers soon before the colonies all run together. Any suggestions, or is it too early to tell?

---

This doesn't look good to me. The Rainbow Eucalyptus print I streaked was extremely
bacterial. I swabbed 3 plates and the first 2 were just nothing but bacterial
goo. The 3rd plate was better and I was able to make 3 transfers. They
are all looking like this pic here, which is exactly the type of myc that I
want.

---

Adas said:

I feel like that's mold. Looks too disorganized for Pan myc.

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Asura said:

rido, the prints have contams. I don't know what your process is, I am just
saying they are there and I think you have a lot more cleaning up to do.

Luv, this stuff looks really nice. I haven't had growth that looked this good
in awhile so I am getting excited about these.

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rido said:

Asura said:

rido, the prints have contams. I don't know what your process is, I am just
saying they are there and I think you have a lot more cleaning up to do.

Luv, this stuff looks really nice. I haven't had growth that looked this good
in awhile so I am getting excited about these.

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Completely agree. I think the fact that all the growths look the same gave a false sense of security that they were all the right thing, instead of all the same contam. I'd be surprised if they were anything other than contam, really.

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A.k.a said:

rido said:

Asura said:

rido, the prints have contams. I don't know what your process is, I am just
saying they are there and I think you have a lot more cleaning up to do.

Luv, this stuff looks really nice. I haven't had growth that looked this good
in awhile so I am getting excited about these.

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Completely agree. I think the fact that all the growths look the same gave a false sense of security that they were all the right thing, instead of all the same contam. I'd be surprised if they were anything other than contam, really.

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He means that getting sterile swabs won’t do anything cuz the contam is in the print. It’s normal that’s what agars for at this point in the process. Its definitely myc in there but it’ll take transfers to get it for sure clean.

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