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OfflineSmoothcat
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26828253 - 07/17/20 06:03 AM (3 years, 6 months ago)

Yeah well done jcm man :thumbup:

I’ll be trying your fruiting chamber out in approx 2 weeks dude for ATL and for pans when I get some spawn together now just waiting on germination.


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InvisiblemushboyMDiscord
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Re: Official Copelandia/Panaeolus Thread [Re: Mateja]
    #26828256 - 07/17/20 06:05 AM (3 years, 6 months ago)

Quote:

Mateah said:
Im so curious to see how they fruit in various HC setups like the Water Tub and what not :rockon:




:stirthepot:


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy]
    #26828275 - 07/17/20 06:33 AM (3 years, 6 months ago)

i use a water tub with 6 airstones an 2 good powered pumps and it works rather well for a small scale grow but if i had the room id rather have a martha/greenhouse type set up

my fc is in my write up😃😃


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PANAEOLUS FRUITING MADE SIMPLE



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InvisibleCosmicGiggle
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Re: Official Copelandia/Panaeolus Thread [Re: mushboy] * 1
    #26828277 - 07/17/20 06:34 AM (3 years, 6 months ago)

Side by side of the cyan Hawaii South point



Tray on the right started out dark like the left tray but they’re approaching white now as the caps start to flatten.

Tray on the left shows no signs of getting lighter in color. There’s a few fruits on this tray that are 24hrs away from harvest and they’re still just as dark as the babies. There’s a few fruits with reddish brown caps but most of them are that bronze-brown you see in the pic.


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: CosmicGiggle]
    #26828333 - 07/17/20 07:17 AM (3 years, 6 months ago)

dude u need to be cloning the shit out of those dark ones and get a good fruiting culture that where that pheno is well stable in the spores cuz i really dig the look of those as im sure will others

they look great


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PANAEOLUS FRUITING MADE SIMPLE



Edited by jcm4620 (07/17/20 07:18 AM)


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26828401 - 07/17/20 08:17 AM (3 years, 6 months ago)

We’re those both isolates


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OfflineAlphaDog52
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26828424 - 07/17/20 08:33 AM (3 years, 6 months ago)





Pan Bama getting ready to be cased today! Super hyped, as this is My first Pan cultivation. Everyone send good vibes please! Will keep updating :smile:


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: AlphaDog52]
    #26828431 - 07/17/20 08:35 AM (3 years, 6 months ago)

looking good so far my man 👍👍


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InvisibleCosmicGiggle
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26828489 - 07/17/20 09:08 AM (3 years, 6 months ago)

To jcm:
I will be making plates tomorrow so I can try cloning them. I’ve only done it once with one of the Beulah fruits and It was super dirty, trying to get it cleaned up now. I’ll make sure I take a bunch from these dark cappers to ensure there’s at least a couple good ones.


To celestial:
Both of these were T2 plates. Definitely not isolates but they fell under the “monoculture” definition as it’s used on here. Uniform growth (no sectors, near-perfect circle), and consistent opacity throughout.

Unfortunately when I dropped my plates to my WBS jars, I forgot to label the jars until I’d already tiger dropped 3 different cultures, so I should have a T4 plate of whichever culture the dark caps came from, but idk which one it is. Only 3 to choose from so it wouldn’t be too big a deal to grow out all three cultures again, except this time label my WBS jars after each drop so I know which one it is lol.


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: CosmicGiggle]
    #26828568 - 07/17/20 09:45 AM (3 years, 6 months ago)

sweet but what i do when cloning pans is to make sure you have a freshly cleaned sab and clean work station and all that jazz lol and yes i still turn off all ac or fans shit like that.i then will tape a piece of thick cardboard onto the lid(bottom working surface of the sab) then on top of that i will tape a sheet of tin foil. il then load my box with all the supplies il need then il wet a paper towel with iso and wipe the tin foil. il then let that evaporate a min then i will take my fruit which went from the fc straight to a ziplock bag and fasten it to the tin foil covered thick cardboard using clean sewing needles with gills facing upward so the stipe is standing up. then i will take a clean pair of scissors and just cut the bottom of the stipe enough to shorten it a lil and give u an entry point to the inside of the fruit so u can peel it apart and downward twds the cap. then with a clean and flame sterilized scalpel i will scrape and remove tissue from where the cap and the stipe meet. sometimes its hard to pick it up but if u cant get it with the scalpel il use the clean and flame sterilized tweezers. to transfer the tissue sample to the plate.

just note that il only go thru all that mess when trying to get a good clean sample from those pan fruits where the stem is so skinny that to even try to take a sample while holding it turns it into a smashed mess 

just thought id share what i do sometimes with those kind of fruits cuz it can be very hard to get a clean sample from them


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26828579 - 07/17/20 09:51 AM (3 years, 6 months ago)

I managed to clone a Bisporus once, from a stem that was like 2mm thick, or even less. While holding it in my fingers. Shit was wild.


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InvisibleZifozonke
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #26828590 - 07/17/20 09:57 AM (3 years, 6 months ago)

@jcm4620

Congrats on your TC Badge & your efforts for spreading//sharing the Pan love to all..:mushroom::mushroom::mushroom::mushroom::mushroom:
:loveheart:


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Zifozonke]
    #26828596 - 07/17/20 09:59 AM (3 years, 6 months ago)

thank you😃😃

and ya them thin bastards can be a pain in the ass man i sometimes wonder how the hell they can even support them big caps sometimes lol


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OnlineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26828608 - 07/17/20 10:06 AM (3 years, 6 months ago)

I gave up trying to get clean pans clones. I’ve just accepted I’ll have bacteria on the first plate now :shrug:

Tiny little bastards.


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #26828624 - 07/17/20 10:20 AM (3 years, 6 months ago)

Quote:

A.k.a said:
I gave up trying to get clean pans clones. I’ve just accepted I’ll have bacteria on the first plate now :shrug:

Tiny little bastards.




Really? I either have bacteria or the cloning works. Nothing in between, vast majority of the time.


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OfflineAsuraS
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620] * 1
    #26828636 - 07/17/20 10:26 AM (3 years, 6 months ago)

Cloning these bastards is so hard. Especially with shitty eyesight.

I like how you pin down the cap, jcm, that's pretty cool. I am always
worried about making multiple cuts and transferring contaminates from
the outer stipe to the inner tissue so I've adopted something that allows
a little leeway for error.

I've been using the agar sandwich method
(which is a variation of a technique called cabin sequestering) and it's worked pretty well. I do it a bit
differently than this write up, but the spirit of it is the same.

It takes two plates. I cut out a square in the middle of a dish and drop a piece of inner tissue directly
to the exposed plate. From the second dish I cut out a bigger square and place it on top of the hole
in the first dish. The idea is the mycelium will grow "out of the box" leaving the bacteria behind.



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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: CosmicGiggle]
    #26828643 - 07/17/20 10:29 AM (3 years, 6 months ago)

You should try to clone it or if there is a small pin try to clone it. The. At least you will have that continuation


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26828670 - 07/17/20 10:42 AM (3 years, 6 months ago)

I’m so excited to see what these guys look like full-grown pan Africa


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26828728 - 07/17/20 11:08 AM (3 years, 6 months ago)

Damn that’s cool I would love to be able to get my hands on e one of those:smirk: can’t wait to see what they will look like


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OfflineLuv_The_Cyans
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1] * 1
    #26828746 - 07/17/20 11:14 AM (3 years, 6 months ago)

I have done a bunch of clones recently. About 50% came out clean and 50% bacterial. Really tough to cut that little sliver out of the stem while holding but possible. I find it always sticks to the blade after and I sometimes demolish the plate trying to get it off.


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