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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Luv_The_Cyans]
    #26828750 - 07/17/20 11:19 AM (3 years, 6 months ago)

With the Bisporus stems I've had, it was impossible to cut anything out. What I found works best is to SCRAPE a couple fibers of the tissue. And yes, getting it off the blade is damn near impossible.. Jesus fuck :facepalm3:


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #26828822 - 07/17/20 11:57 AM (3 years, 6 months ago)

lol ya it can be a major pain in the balls for sure and i dread doing it sometimes when i have those kind of fruits. that is why i try to get the tissue that is in the middel from inside the cap just to where it becomes hollw. that way i can usually get actual meat and not just threads that are even more a pain in the ass as duley noted above


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OfflineLuv_The_Cyans
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26828836 - 07/17/20 12:04 PM (3 years, 6 months ago)

Thanks for the tip JCM :takingnotes: I know for next time!


Edited by Luv_The_Cyans (07/17/20 12:05 PM)


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26828837 - 07/17/20 12:05 PM (3 years, 6 months ago)

Cloning pans is tricky but with practice it can be done consistently when I first started my success was only about 5% I would make at least five attempts of every clone now I succeed with about 80% of my clone attempts practice makes perfect be sure to heat your blades several times throughout the process


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26828848 - 07/17/20 12:10 PM (3 years, 6 months ago)

but i may just give that a go asura id never heard that one before and i never thought of such a thing i just tacked it down like i was disecting a frog in high school lol


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26828862 - 07/17/20 12:15 PM (3 years, 6 months ago)

Quote:

Jakeoncid419 said:
Cloning pans is tricky but with practice it can be done consistently when I first started my success was only about 5% I would make at least five attempts of every clone now I succeed with about 80% of my clone attempts practice makes perfect be sure to heat your blades several times throughout the process





hell yes very important

but what i like the most about tacking it down like that so the stipe is vertical is that it allows me to pull the stipe apart without too much touching and damage. but no matter what u do its a pain in the ass for sure.


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620] * 1
    #26828894 - 07/17/20 12:33 PM (3 years, 6 months ago)

I just use tweezers and a scalpel, Cut it vertically down the stipe then Hi lift of small strand with the tip of the blade grab it with the tweezers and pull it free, Then with flame sterilized scissors I cut the strip free of the tweezers over the dish so it drops onto the agar without having touched anything else


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26828905 - 07/17/20 12:39 PM (3 years, 6 months ago)

ya i need to get a good stainless steel pair of fine point scissors i just always keep forgetting them and everytime i end up needing tbem i think fuck how come i dont have any lol


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OfflineCelestialexplorer1
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26828948 - 07/17/20 12:57 PM (3 years, 6 months ago)

Hey ak you had asked if anyone ever sterilized manure and nocced it before. This is 5 days old and 50%. It is Hpoo 35% straw 60% a tiny bit of Verm, some grass seed and wheat berries 5%-8%. Pced for 4 hours because it was in an instant pot(it’s just so much more convenient than pulling out my big one. I have 4 other qts just like it. All tropicalis

Also Asura and i believe blue helix both have a lot of info on doing this in mycobags as long as a lot of other people.


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Edited by Celestialexplorer1 (07/17/20 01:02 PM)


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OfflineA.k.aM
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26828979 - 07/17/20 01:12 PM (3 years, 6 months ago)

The square thing is cool, I’ve seen the sandwich before but not taking a piece off to start.

Most of the time I get bacteria with around half of the tissue and myc on the rest. Other times it’s just all bacteria but if you give it a week or so the myc will pop through and outrun it. I was worried about bacteria hitching on the myc but I’ve done it quite a few times with no noticeable problems yet.

I usually do a few transfers from bacterial tissue first before using it though so maybe there was some riding along on the early plates.


I’ve got 4 wild coast tubs going now, one about to be cased. Still have enough spawn to make 3-4 more. Now I gotta dig up my jam plates and get those going too.


My previous grows were all steer manure compost mixed with verm, this time I have one of those, one coir, and two 50/50 coir manure. Today’s agenda is hay/coir and hay/manure.


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Celestialexplorer1]
    #26828995 - 07/17/20 01:20 PM (3 years, 6 months ago)

ya blue helix and asura both sterilize an all in one style bag and knock up via LC


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OfflineAlphaDog52
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26829026 - 07/17/20 01:31 PM (3 years, 6 months ago)









Casing layer look ok to you guys?


Edited by AlphaDog52 (07/17/20 01:32 PM)


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: AlphaDog52]
    #26829057 - 07/17/20 01:44 PM (3 years, 6 months ago)

too hard to really tell with those pics but ate u just planning on fruiting those in propagation domes??


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OfflineJakeoncid419
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Re: Official Copelandia/Panaeolus Thread [Re: AlphaDog52]
    #26829066 - 07/17/20 01:47 PM (3 years, 6 months ago)

Oh I meant to tell you guys a few weeks ago, So I had been having a lot of trouble with my bulk contaminating after I add my spawn. now at first I thought it was my manure quality and part of it was as the stuff I was using for a little while there was total garbage but now I have my optimal Pooh and I was still having problems with my pasteurized trays almost all of them were contaminating I had changed nothing about my process the only difference is the season so for the last month and a half or so I’ve been sterilizing my bulk and Myco bags then inoculating with grain. It’s Working really well so far the reason I normally stuck with pasteurization was because in my experience those trays just seemed to resist contamination longer so far these trays are holding up about the same amount of time 2.5 flushes average. I would imagine it has something to do with The summer air and it finding its way in through small cracks in the trays while they’re colonizing (pasteurized tek). So yeah if you find yourself running into contamination after contamination switching over to the sterilized  bags is a good way to isolate your problem.


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26829074 - 07/17/20 01:51 PM (3 years, 6 months ago)

so ur sterilizing your grain and bulk in sepperate bags and spawning to the sterile sub bags?? thats what your saying right?


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OfflineAlphaDog52
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26829089 - 07/17/20 01:57 PM (3 years, 6 months ago)

Quote:

jcm4620 said:
too hard to really tell with those pics but ate u just planning on fruiting those in propagation domes??




No, I'll be moving this tray and stand into a 106 qt tub that I have equipped with the adjustable water heater, and I'm running 5 air stones into the bottom. I've also gotten an ultrasonic cool mist to pipe into the tub for assisted humidity control. Almost identical FC setup as yours. I'm letting this consolidate the casing layer for about 18 hours, and it will be going into the FC.


Edited by AlphaDog52 (07/17/20 01:59 PM)


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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26829093 - 07/17/20 01:59 PM (3 years, 6 months ago)

Another convert to sterilized bulk. Welcome :lol:

I've been thinking that I want to try inoculating quart sized jars of sub now.
I could chop up a whole plate and do a jar for each sector and then fruit in
a bunch of small containers. Nice for clone hunting.


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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #26829101 - 07/17/20 02:05 PM (3 years, 6 months ago)

Yeah my only issue with the Myco bags is the ones on bottom taking on extra water and throwing off the moisture content which is very important if it’s too wet it will kill the Spawn, But I’ve started taking the bottom two rows of bags and double bagging them two at a time so I make the bags up fold my bags then slide two of those bags into another Myco bag which I then vacuum seal and layer into my pressure cooker. Of course making the bags can sometimes be a pain the smallest air bubble can cause problems but I don’t have many of those issues anymore my issue is always losing the bottom two bags but it seems I have a fix that also the bags you choose make a big difference some of them are just way too thin I got a big box of the good thick XL bags so I’ll probably stick to this method for a while at least until winter I am interested to see if the pasteurized method Will start working again in the fall


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online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: Jakeoncid419]
    #26829186 - 07/17/20 02:51 PM (3 years, 6 months ago)

alpha- right on man just was wondering cuz i use the prop domes too but i just use  the trays in my fc for fruiting and il use the domes on them so i can see them while they recover and then consolidate. so were on the same page there and im glad that ul be using my fc syle too😃😃  so plz post ur results in my write up too so that way me and others can see how it works out. the only reason i asked was cuz i have fruited cubes in the domes no prob. but pans dont do good at all in them👍👍


but asura i have also used sterilized bulk in bags and had about the same results as i do with my pasteurized sub. and im also thinking of givin that a go next year when i run out of my made and frozen substrate lol but i also need to get out and get more small trays for fruiting in for the purpose of clone hunting. my other ones melted on me when they were in the dishwasher lol but its a lot of work fruiting out all those tiny grows but it beats runnin full trays of shit lol👍😃 

jake thank u bro i just went to my buddys place and picked up my prints that ya sent👍👍 thank u again very much. if there is wver something from me u want just ask👍


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Offlinejcm4620
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Re: Official Copelandia/Panaeolus Thread [Re: jcm4620]
    #26829262 - 07/17/20 03:32 PM (3 years, 6 months ago)

lol well now that i dont have nothing special to look at right now here is a close up view of the tray i harvested yesterday its in segments but its close to the whole tray.😃😃



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