Psilocybe cubensis

Home | Community | Message Board

You are not signed in.
New Account

Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation > Mushroom Cultivation Archive > Grow Logs

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop:

Bulk Cannabis Seeds

Isolated Cubensis Liquid Culture For Sale

Liquid Cultures

Spores for European Microscopy

All-In-One Bags That Don't Suck

Injection Grain Bag

Jump to first unread post

Pages: 1

Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.

socratesmind
old hand

Registered: 02/22/02
Posts: 1,193
Loc: in your house :)
Last seen: 20 years, 2 months

Psilocybe cubensis "Puerto Rican" PF-Tek

#1003259 - 10/29/02 01:15 PM (23 years, 10 months ago)

A friend of a friend sent me this thru the email and is a great fictional story about a man in the early 70's cultivating. Very interesting and she said she'd send the rest in future emails. No partys discussed here in are results from my experiences.

9/30- sometime around this date i make the syringe using a shot glass a print and a knife and tin foil. flame sterilized the knife with butane lighter. liberally sprayed work area down with lysol and cleaned surface with 91% isopryl alcohol. opened up print and scraped spores into shot glass and covered with tin foil. sterilized syringes were stuck thru the tin foil to minimize draft (i'm sure tin foil wasn't best material to use but it was available.) also flame sterilized needle and then shot water into shot glass and sucked back up and did it again to get any missed spores. shot glass was also round and very cylindericular. so no spores would be caught on side of glass. put needle guard on needle and let it sit in a cool (70-75F) dark place.

10/22- sterilize 6 1/2 pint kerr wide mouth jars using the pf max fruting formula @ 10psi 20 mins. 4 innoc points with a 3 penny nail in the outer rim of jars. sealed with mascon tape. one layer of tin foil on top all pre-sterilization and after steriliziation i smelt a peculirar smell and decided to remove tinfoil and dab off excess water from on top of tape. next day it was dry.

10/23- open air innoc with home made pr syringe print courtesey of a friend of a friend. lysol'd down room before entering bathroom and then heavily lysoled bathroom down and cleaned surfaces with a 10% bleach/water solution. waited for lysol to settle before flame sterilizing to avoid flash fire say 10 minutes. flame sterilized needle with butane lighter that left black soot. flame sterilized on every 2 jars to ensure no cross contamination. innoc total 6 jars removing tape off one hole at a time and replacing. why 6? only had 6cc's in syringe and still got like 2 left so do the math yourself thats like under .25cc per hole but pr is a great colonizer from what i hear. incubate in closet around 70-74F with tape over holes and no tin foil with lids on loosely.

10/28- built incubator today with cooler/bottle of hot water and cover up top of cooler with sheet. temp is around 84-75. growth already spotted around 4 innoc points without incubating and temps around 73. gotta refill water bottle a few times a day. Temp starts at as high as 86 and cools to around 78 then refill with hot water and do again.

10/29- mycellium under 4 innoc points is size of a quarter. no mold visible in any jars. also see water droplets around mycellium very tiny.

11/3-mycellium is very rhizomorphic. tiny water droplets above the mycellium. very close to all 4 points touching each other. maybe 1-2mm away and still being incubated from 86-72. (i'm falling behind on the refillings.) any ideas on when to expect say full colonization? guy who sent me spores sayd 20 days full colonization with this pr? wish i had pics :frown:

11/5- approaching 100% colonization and i flipped jars today. bottom is almost fully covered with mycellium so wait one week past the bottom covering? Also remove tape as well or ? This is the part where i usually messup :frown:

11/6- some are almost 100% colonization just a few mm on bottom of jar and removed tape. in next few days i'm going to start exposing them to light. seems already that i see balls of mycellium though or hyphal knots??? possible?

11/8- switched incubator to a bigger cooler. inside incubator i introduce a 4w light bulb to start getting pinning initiated and going to leave the light on for 24/7 to maintain heat and expose jars to light. only 4w so shouldnt generate too much dry heat. jars are flipped and removed tape. near the verm barrier there are signs of limited growth? maybe the pr doesnt like the dry verm. also innoc'd two karo jars last nite. karo jars are also stored in incubation chamber and are wrapped in tin foil the sides as to know let light expose them to the growing mycellium. also mycellium is extending to the outside of the jar as if the cake kinda shrunk some but the rhizomorphs are going out to jar? rhizomorphs or hyphal knots? Terrarium is pre setup as a 72 quart rubbermaid supported under it with 8 dog food jars scored for $2 :laugh:

and a 1/2" hole in bottom to allow draining and co2 evaporation. Thanks for idea shroomologist. Also sterlite container below hole to catch drippings.

11-12 -birthed two cakes tonite and going to wait on the other 4 to invitro pin. Shookoff most of verm layer and gave it a few sprinkles of water and set it down on some tinfoil. Was going to dunk but didn't feel like it. Fruiting chamber is a 72Q rubbermaid with a light in top. Also perlite for humidifcation and hole in bottom. Temp around 72.

11-14 - two cakes still chiling with 3 total tubs of perlite in the fruiting chamber no signs of pins yet but just got the hygrometer tonite and it says HI% so i guess thats a good sign. the light doesnt seem to dryout the chamber either. one cake may either show a sign of contam or blueing?? dont know which but will probably go ahead and birth other 3. fruiting chamber temp is 68-72. and hi RH since working with cakes.

11-19 - 4 cakes still sitting in fruiting chamber and fanning 3-4x a day and no signs of pins yet. still hoping as it hasn't been past 7 days. temp is hovering around 68-72 still as well. if pinning goes past 10 days and i see nothing ima be a lil worried then. should i? also cased the other 2 colonized cakes today using hongus tek without the bottom layer and crushing the cakes in ziploc bag and putting in container and covering with foil with a few holes in foil and placed back in incubator. which has now changed to a large cooler with a 4QT submersible heater set on 82 and keeps the temp around 80 or so inside the cooler.

11-21- removed casing from incubator as it had 2 small patches of mycellium poking thru it. into the refrigerator it goes for about 8 hours. it comes out and gets put in saran wrap to fruit unlike the terrarium as terrarium RH is too high for casing.

11-25- casing is showing growth and going to patch her up. ditched out on cakes and praying for outdoor gods to helpout. damn i hate failure. :frown:

next time i guess wait to pin invitro?

12-3-well results we have failure is not. casings have about 6 baby pins growing thru middle of casing layer that was patched. casings are way better than cakes imho. will let know flush weight later.

12-11- well picked of last of mush tonite and total yield from this picking was around 2g dry how lame i know right. first sucessful grow and so stoked. mush were picked since i noticed a funky brown wet looking contam coming over the casing layer. so i decided to pick and have those instead of not having nothing at all. so sucess and next time i'll be more careful with casing and actually microwave the verm before applying. this grow log is complete.
too bee continued.

Edited by Annom (12/21/04 10:38 AM)

Extras:

Similar Threads	Poster	Views	Replies	Last post
Psilocybe cubensis "Puerto Rican" on rye/compost ( 1 2 all )	Joshua	12,853	35	04/17/02 05:08 PM by Joshua
Psilocybe cubensis "wild print" pf-tek ( 1 2 3 all )	niteowl	21,024	47	12/06/03 11:48 PM by niteowl
Psilocybe cubensis "Treasure Coast" PF-Tek cased ( 1 2 3 all )	Split	20,383	58	03/01/02 11:19 PM by Oliver
Psilocybe cubensis "Puerto Ricans" on rye/compost ( 1 2 3 4 5 6 7 all )	Joshua	28,559	130	03/21/02 03:09 PM by Joshua
Psilocybe cubensis "Matias Romero" PF-Tek - Cased non-sterilized coir ( 1 2 3 4 5 6 7 8 all )	Sir Tokes-A-Lot	29,131	156	11/28/01 10:37 PM by Sir Tokes-A-Lot
Psilocybe cubensis "PR" mycobags, fruited in bags. ( 1 2 3 all )	c0wb0yfr0mh3ll	27,168	54	02/24/02 11:42 AM by SouthernGent
Psilocybe cubensis "B+" PF-Tek ( 1 2 all )	plura16	23,731	27	12/15/03 08:31 PM by Walrus_Infested
Psilocybe Cubensis "Malabar" PF-Tek spawned to uncolonized BRF, large flat cake Fruited on cake	u4ia	13,039	10	04/19/04 09:26 PM by daywalker