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Geezed
Blessed

Registered: 04/15/23
Posts: 74
Loc: Lost Lands
Last seen: 10 days, 3 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: BeefExtreme]
#29361330 - 10/01/25 01:27 PM (10 months, 17 days ago)

BeefSupremeJr said:

hi aygar buds. i done posted episode 4 of beeftube and Mojave Myc guest starred. we did a ton of agar stuff. please peep it and lmk what ya think.

Does anyone still sterilize there tools in a PC anymore?

Like scalpels wrapped in foil? Or is alcohol & flame sterilization good enough?

--------------------
Learning from cultivation knocks - human error
The Beeftube - getting the beef

Edited by Geezed (10/01/25 01:31 PM)

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mushboy
modboy

Registered: 04/24/05
Posts: 40,443
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Geezed]

1
#29361346 - 10/01/25 01:40 PM (10 months, 17 days ago)

ill sterilize qtips and certain tools ill want clean like loops to dip in lc for testing but i havent pc'd a scalpel in ages.

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:hypnotoad:

:hypnotoad:

Making Liquid Culture : Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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flapping_bird
Human, Be-ing

Registered: 01/03/25
Posts: 1,765

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Geezed]

9
#29361361 - 10/01/25 01:52 PM (10 months, 17 days ago)

A few pics to share, taken with the aid of an el-cheapo but good enough digital microscope.

Cubensis mycelium bridging the 3mm gap of an agar trench, on MEPA. In the background of the trench is the black metal of scope stand/base. 2025

A dried colonized whole oat grain inside of an agar "cabin", on MEA, rehydrating, about 48 hours after placing onto agar. 2025 [Thanks for sharing your dry grain storage method, Fahtster!]

TTBVI clone, on MEA, Sept 2025

Semperviva clone, on PDA, bacterial but clean enough to txfr and capture. Sept 2025

This sure is a fun hobby, with a lot of great folks involved. I appreciate all of the sharing of knowledge and encouragement. Best wishes for peace to all!

(the edit was addition of "inside of an agar cabin" on the oat grain pic. description)

--------------------
Peace to you, friend!
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Edited by flapping_bird (10/01/25 01:55 PM)

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Gilmour Hendrix
Village Idiot

Registered: 08/02/25
Posts: 1,644
Loc: Bacta-Mold HQ

Re: AGAR ENVY! (Anything and All things agar!)

[Re: flapping_bird]

1
#29361372 - 10/01/25 02:02 PM (10 months, 17 days ago)

Neat pics!

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Dala
Rad Visuospatial Sketchpad

Registered: 11/23/08
Posts: 2,913
Loc: Caddy-Corner to Quality

Re: AGAR ENVY! (Anything and All things agar!)

[Re: flapping_bird]

1
#29361535 - 10/01/25 05:03 PM (10 months, 17 days ago)

Stunning pics flapping_bird :super:

:super:

Have you heard of storing bacterial plates vertically? The myc can fight gravity and climb upwards, but the bacteria can't.

fahtster said:

https://www.shroomery.org/forums/showflat.php/Number/29113113#29113113

ReverendMyc said:

https://www.shroomery.org/forums/showflat.php/Number/29320208#29320208

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MUSHROOMS RULE

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flapping_bird
Human, Be-ing

Registered: 01/03/25
Posts: 1,765

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dala]

1
#29361604 - 10/01/25 06:01 PM (10 months, 17 days ago)

Dala said:

Stunning pics flapping_bird

Have you heard of storing bacterial plates vertically? The myc can fight gravity and climb upwards, but the bacteria can't.

Thanks! They were fun pictures to be able to capture. ***ESPECIALLY*** that semp clone pic, with thanks to you! I feel like I’ve won the lottery on that one, just getting to the point of being able to take the sample to put to agar. Excellent resources, thank you! That’s a golden tip. I haven’t used PDA much (only this current batch of plates) and surely it can’t be anything specific to PDA, but I have noticed on a few similarly dirty clone samples on PDA that the myc eventually seems to gain the ability to outgrow and ?kill?/?encapsulate? / ?overgrow? the baccy spot and continue on it merry way, looking pretty good in spite of it.

Many thanks & peace to you.

--------------------
Peace to you, friend!
The HHG is a guide to getting started.
New User Welcome Wagon
Cake Wars II: Ends 06/30/2026
GROWER’S BEST photo contest: join the fun!
CAKE WARS III - Starts SEPT 1, 2026

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Dala
Rad Visuospatial Sketchpad

Registered: 11/23/08
Posts: 2,913
Loc: Caddy-Corner to Quality

Re: AGAR ENVY! (Anything and All things agar!)

[Re: flapping_bird]

1
#29361612 - 10/01/25 06:11 PM (10 months, 17 days ago)

You're doing awesome work I love it

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MUSHROOMS RULE

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ihansterx4i
Stranger

Registered: 04/26/25
Posts: 577
Last seen: 2 hours, 18 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Dala]
#29362167 - 10/02/25 10:10 AM (10 months, 16 days ago)

Can i put 1 grain from a grain jar im putting into spawn on agar and grow that out? Just do it carefully in front of a flow hood? I know you can grain to grain but I don't know yet If i want another jar going quite yet. But I also want to have it on hand ready to push to grain if I wanted to.

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,339
Loc: Exact Center
Last seen: 9 days, 2 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ihansterx4i]
#29362170 - 10/02/25 10:14 AM (10 months, 16 days ago)

Yes.

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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After you know what you're doing, take a break
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Geezed
Blessed

Registered: 04/15/23
Posts: 74
Loc: Lost Lands
Last seen: 10 days, 3 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#29362177 - 10/02/25 10:23 AM (10 months, 16 days ago)

:takingnotes:

--------------------
Learning from cultivation knocks - human error
The Beeftube - getting the beef

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ihansterx4i
Stranger

Registered: 04/26/25
Posts: 577
Last seen: 2 hours, 18 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#29362179 - 10/02/25 10:25 AM (10 months, 16 days ago)

:bow2:

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Packerbacker12
When it rains it spores

Registered: 08/26/18
Posts: 245
Last seen: 5 days, 6 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ihansterx4i]
#29362233 - 10/02/25 11:35 AM (10 months, 16 days ago)

I just got some 500ml media bottles and I'm gonna make agar in them for the first time. When I take them out of the PC, is it safe to tighten the lids down then?

I thought I've read that will create a vaccum. I plan to use them as soon as they're cool enough to pour but might want to make some ahead of time for the future.

I'm probably overthinking this but I'm used to cooking agar in containers that have filtered lids.

--------------------

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,082
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Packerbacker12]
#29362238 - 10/02/25 11:38 AM (10 months, 16 days ago)

I answered in the other thread.

Stipe-n Cap said:

Packerbacker12 said:

I just got some 500ml media bottles and I'm gonna make agar in them for the first time. When I take them out of the PC, is it safe to tighten the lids down then?

I thought I've read that will create a vaccum. I plan to use them as soon as they're cool enough to pour but might want to make some ahead of time for the future.

I'm probably overthinking this but I'm used to cooking agar in containers that have filtered lids.

You can finger tighten them to move into your work space, then back the lid off again. The threads act as a barrier to contamination. Don't tighten them while piping hot or your agar will begin to boil.

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funkmastakush
Arachnid Promoter

Registered: 08/24/14

Posts: 200
Loc: Places
Last seen: 11 days, 30 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]

3
#29362749 - 10/02/25 08:54 PM (10 months, 16 days ago)

First few times messing with agar and I think I got some decent plates going! These are the first transfers from original plates (don't mind shitty labeling). PC GT. Having decent luck lately with little contamination! Thanks, Shroomery!

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Edited by funkmastakush (10/02/25 08:55 PM)

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Kloakk
Strange

Registered: 10/21/24
Posts: 5,423
Loc: Scandinavia
Last seen: 15 minutes, 7 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: funkmastakush]
#29362781 - 10/02/25 09:22 PM (10 months, 16 days ago)

funkmastakush said:

First few times messing with agar and I think I got some decent plates going! These are the first transfers from original plates (don't mind shitty labeling). PC GT. Having decent luck lately with little contamination! Thanks, Shroomery!

Send them to grains :rockon:

:rockon:

--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett

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funkmastakush
Arachnid Promoter

Registered: 08/24/14

Posts: 200
Loc: Places
Last seen: 11 days, 30 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Kloakk]

1
#29362871 - 10/02/25 11:10 PM (10 months, 16 days ago)

Kloakk said:

funkmastakush said:

First few times messing with agar and I think I got some decent plates going! These are the first transfers from original plates (don't mind shitty labeling). PC GT. Having decent luck lately with little contamination! Thanks, Shroomery!

Send them to grains

Thanks man, I plan to send these to several WBS or oat jars very soon! Need to learn much more about agar... I barely know anything about it lol. Just squirted some MS onto these plates and seem to be doing OK. LOVE this hobby! Just wish work didn't consume our time as I would love to devote all my time to this awesomeness! Not enough hours in the day lol

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neonero
Stranger

Registered: 03/25/20
Posts: 64
Last seen: 8 months, 23 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: BeefExtreme]
#29362946 - 10/03/25 02:44 AM (10 months, 15 days ago)

Thank´s for posting @BeefSupremeJr
I am just learning this, so it´s very helpful for me.

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ihansterx4i
Stranger

Registered: 04/26/25
Posts: 577
Last seen: 2 hours, 18 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: neonero]
#29363772 - 10/03/25 09:04 PM (10 months, 15 days ago)

Could anyone explain what’s happening to my ochra transfers from spores?

2% agar and 2% LME plates.

I was told regarding it growing up that it was too much nutrients but what is happening with the juice?

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,339
Loc: Exact Center
Last seen: 9 days, 2 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ihansterx4i]

2
#29363778 - 10/03/25 09:16 PM (10 months, 15 days ago)

Possible mycogone perniciosa.

Orange or yellow liquid are enzymes produced by the myc known as metabolites.

Typically excreted in microscopic amounts for exploration of surroundings, digesting food, and in this case, dealing with stress, likely from contamination

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

ihansterx4i
Stranger

Registered: 04/26/25
Posts: 577
Last seen: 2 hours, 18 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#29364399 - 10/04/25 04:52 PM (10 months, 14 days ago)

Do you think I need to keep isolating until I get a clean uniform growth?

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