Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

PlanetSpores.ca
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Mono Tub Substrate   North Spore Injection Grain Bag

Jump to first unread post Pages: < First | | 1395 | 1396 | 1397 | 1398 | 1399 | 1400 | 1401 | 1402 | 1403 | 1404 | 1405 | 1406 | 1407 | 1408 | 1409 | 1410 | 1411 | 1412 | 1413 | 1414 | 1415 | | Last >
Re: AGAR ENVY! (Anything and All things agar!) [Re: sage_bd]
    #29115823 -

LewDoja said:
Not necessary.
But are there benefits, probably.


Tnx. What would those be, besides what sage_bd said below?

sage_bd said:
I don't think the wood does anything for cubes, but it does seem like a convenience thing. It seems like it would be easier to work with since you can pull the stick out with sterile tweezers then grab a transfer.

I have slants with popsicle sticks in them but the only thing I have on them right now is wild turkey tail which I think wood is probably beneficial for. Make sure you hydrate/sanitize the sticks in boiling water before use.


Tnx mate. I don't know if I'll be using sticks though. Unless I hear a good reason to.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: koma23]
    #29115826 -

And when we're on the topic of slant's, has anybody ever made small, micro slants using these? I really don't have much space in my fridge ... I'm wondering really, what are the benefits of the bigger centrifuge tubes vs agar plates anyway, except that centrifuge tubes close them self's up better and thus maybe don't let as much moisture through (all my agar plates in the fridge end up having water in them, in spite of having them locked up in sealed Tupperware).


Edited by koma23 (02/06/25 03:09 PM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: koma23]
    #29115828 -

koma23 said:
And when we're on the topic of slant's, has anybody ever made small, micro slants using these? I really don't have much space in my fridge ... I'm wondering really, what are the benefits of the bigger centrifuge tubes vs agar plates anyway, except that centrifuge tubes close them self's up better and thus maybe don't let as much moisture through (all my agar plates in the fridge end up having water in them, in spite of having them locked up in sealed Tupperware).




I've got a whole bag of those and the little stand thing they fit in
Still never used a single one


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.
Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme] * 1
    #29115832 -

BeefSupremeJr said:
I would only use the Pyrexx. I have heard of bottles exploding in people's hands due to rapid temperature changes. Beyond trying to give people good growing advice, my number one priority has to be safety.


I know you don't need this explained to you but just putting this out there.

Rapid temperature changes with glass should be avoided even if you do have pyrex. I boiled out all of my solvent in one of my labs during college and tried to add some water back to the blazing hot pyrex beaker and it shattered (obviously).

Glass isn't designed to handle rapid temp swings. It's a poor thermal conductor and isn't a malleable material (unlike metals which still warp from rapid temp swings).

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: koma23]
    #29115838 -

koma23 said:



My guess is that these would be too small to serve the purpose that slants serve.

At least in my understanding (which is limited) the purpose of slants is to preserve myc for an extended period of time and having a 15mL tube slant with approximately half that filled with agar and slanted, it gives a large pocket of nutrition for the myc to feed off slowly over the next 6 months to year before folks recommend performing transfers to maintain viability.

So by using those tiny centrifuge tubes, you don't provide many nutrients to the myc for preserving it over a long period of time.

But anyone feel free to correct me here, just speaking from my limited understanding.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: sage_bd] * 2
    #29115848 -

I initially bought them thinking I would try some distilled H2O storage, or potentially storing dried grain


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.
Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: koma23] * 1
    #29115849 -

koma23 said:
And when we're on the topic of slant's, has anybody ever made small, micro slants using these? I really don't have much space in my fridge ... I'm wondering really, what are the benefits of the bigger centrifuge tubes vs agar plates anyway, except that centrifuge tubes close them self's up better and thus maybe don't let as much moisture through (all my agar plates in the fridge end up having water in them, in spite of having them locked up in sealed Tupperware).




Might be able to use eppendorfs for something like Cryovial Tek


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope] * 1
    #29115861 -

sage_bd said:
koma23 said:



My guess is that these would be too small to serve the purpose that slants serve.

At least in my understanding (which is limited) the purpose of slants is to preserve myc for an extended period of time and having a 15mL tube slant with approximately half that filled with agar and slanted, it gives a large pocket of nutrition for the myc to feed off slowly over the next 6 months to year before folks recommend performing transfers to maintain viability.

So by using those tiny centrifuge tubes, you don't provide many nutrients to the myc for preserving it over a long period of time.

But anyone feel free to correct me here, just speaking from my limited understanding.


I mean, mycelium in the fridge doesn't use up much nutrients as it's growth is more or less stalled, so, idk how much nutrients it really needs?

Camera93 said:
I initially bought them thinking I would try some distilled H2O storage, or potentially storing dried grain


Dried colonized grains you mean?

Scemilope said:
koma23 said:
And when we're on the topic of slant's, has anybody ever made small, micro slants using these? I really don't have much space in my fridge ... I'm wondering really, what are the benefits of the bigger centrifuge tubes vs agar plates anyway, except that centrifuge tubes close them self's up better and thus maybe don't let as much moisture through (all my agar plates in the fridge end up having water in them, in spite of having them locked up in sealed Tupperware).




Might be able to use eppendorfs for something like Cryovial Tek


Ah, there are sooo many ways to go about anything in mycology ... If mycelium can be stored in distilled water without any nutrients, outside of a fridge, then why the heck would you want to bother with slant (I understand slants have benefits) ... Appreciate the link!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: koma23] * 1
    #29115867 -

I think there are better ways (than slants) to store long term, here are a few links I'm aware of.

Easy LI for Preservation and Propagation
Cryogenic storage of P. cubensis mycelium, or can I freeze my cultures?
The OFFICIAL Stasis Storage Thread

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: koma23] * 1
    #29115868 -

koma23 said:
And when we're on the topic of slant's, has anybody ever made small, micro slants using these? I really don't have much space in my fridge ... I'm wondering really, what are the benefits of the bigger centrifuge tubes vs agar plates anyway, except that centrifuge tubes close them self's up better and thus maybe don't let as much moisture through (all my agar plates in the fridge end up having water in them, in spite of having them locked up in sealed Tupperware).




I’ve used these to send wedges of agar with culture, and have received these from others. I have a bunch that are almost a year old with usable cultures still inside. I’ve stopped using slants. There are other easier ways to store cultures.


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: sage_bd] * 2
    #29115870 -

Welll anythings possible but pyrex is designed to handle some major temp
swings.  think about glass-top stoves. 

im not getting anywhere near 40 plates at 1500ml.  i could count them later when i get home but i think thin plates suck.  they dry out too fast and they tend to stick to the walls of jars bad.  i like thicc plate.


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme] * 3
    #29115880 -

Agar obesity is an epidemic


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: koma23]
    #29115883 -

Seeing all these great looking plates in this thread makes me question my capability and methods - I'm never really 100% satisfied with every plate or every poor and I wonder if this is similar for everyone.  So far I've only worked in a SAB, methods I've tried - rated from memory:
- Started with disposable plates and pour method - great for clarity, good for condensation, fair for contam (bad for environment!)
- Tried reusable glass petris/pour method - great for clarity, fair for condensation and contam
- Tried PP5 90 mm  petris/pour method - good to fair (opaqueness with reuse) for clarity, good for condensation, fair for contam
- Moved to no pour with the glass/PP5 90 mm - fair (opaqueness with resue) for clarity, fair for condensation, good for contam; also occasionally had trouble with agar (re)solidification
- Latest choice is using the 'Primo Plates', no pour tek with 60 mm clear Viabooth petris - great for clarity, fair for condensation, great for contam with nice benefit of bullet proof storage prior to use.

I recognize that some of the contam issues may be my SAB technique (big, clumsy hands:rolleyes:).  Some questions: Are my expectations too high? For those that are satisfied with their method of choice - are you still pouring 15 to get 10 good plates as an example?  Also, if you started on your journey in SAB with no-pour then moved to FFU did you go back to pour method with improvement on plate condition/yield?

I'd gladly shell out for an FFU in exchange for 100% good plate pours.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: catnip40] * 8
    #29115886 -

catnip40 said:
Agar obesity is an epidemic


Chobese wedges 🥵

BBW = big beautiful wedges 🍆 💦 😳

I like em thicc, slick, and ready to flick. Xfer? XXXfer, more like it 😈


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: MetalPro]
    #29115890 -

Maybe it’s just me but I didn’t quite get if your main problem is getting contamination even with plates you haven’t even used yet, or problems after inoculation, transfers? This probably influences the discussion quite a bit.

Edit: Just as a data point I just started with agar. Have poured 60 plates in a SAB (mostly because I’m quite paranoid about what I’m transferring). And I have had two unused plates that had something on them, and I’m not sure if one bacterial weirdness wasn’t just introduced during a transfer.

Edited by eilauver (02/06/25 04:32 PM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme] * 2
    #29115891 -

BeefSupremeJr said:
Welll anythings possible but pyrex is designed to handle some major temp
swings.  think about glass-top stoves. 

im not getting anywhere near 40 plates at 1500ml.  i could count them later when i get home but i think thin plates suck.  they dry out too fast and they tend to stick to the walls of jars bad.  i like thicc plate.


Agreed and the only time I'll ever pour thin plates is 60 ml dishes I plan to use for lc ( I get less condensation with thin plates with the 60 clavables)


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: RoscoeReturns] * 1
    #29115959 -

RoscoeReturns said:
koma23 said:
And when we're on the topic of slant's, has anybody ever made small, micro slants using these? I really don't have much space in my fridge ... I'm wondering really, what are the benefits of the bigger centrifuge tubes vs agar plates anyway, except that centrifuge tubes close them self's up better and thus maybe don't let as much moisture through (all my agar plates in the fridge end up having water in them, in spite of having them locked up in sealed Tupperware).




I’ve used these to send wedges of agar with culture, and have received these from others. I have a bunch that are almost a year old with usable cultures still inside. I’ve stopped using slants. There are other easier ways to store cultures.


:whathesaid:

They dont even need to be in the fridge.
I've had better luck with older wedges in these ,Than I've had with newer cultures sent in Dh20.

Edited by LewDoja (02/06/25 05:01 PM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LewDoja] * 5
    #29115972 -



Got 16 lc's tested on agar today now to make a couple hundred transfers lol


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: eilauver]
    #29115977 -

eilauver said:
Maybe it’s just me but I didn’t quite get if your main problem is getting contamination even with plates you haven’t even used yet, or problems after inoculation, transfers? This probably influences the discussion quite a bit.



I've gotten a few plates with what is clearly edge contamination when using the pour methods, I haven't been sealing after pour/before use but I have stacked and covered them in the SAB. I've also gotten the occasional random splotch which I attribute to my SAB/hand technique when pouring(all before use).  I didn't consider these a huge problem - just another factor that lowered my yield of good, usable plates from a pour session or technique.  With the Primo Plates tek the contam issues have all gone away but condensation is not satisfactory (for me). Were all 60 of your SAB pour plates usable?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr]
    #29115983 -

cronicr said:


Got 16 lc's tested on agar today now to make a couple hundred transfers lol


:eek:

What are the plates generally?
Stacks of copies of the same culture, or all unquie cultures?


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.
Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can

Extras: Filter Print Post Top
Jump to top Pages: < First | | 1395 | 1396 | 1397 | 1398 | 1399 | 1400 | 1401 | 1402 | 1403 | 1404 | 1405 | 1406 | 1407 | 1408 | 1409 | 1410 | 1411 | 1412 | 1413 | 1414 | 1415 | | Last >

Shop: MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Mono Tub Substrate   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* buying agar - help sinisha 3,839 6 03/31/06 07:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 8,398 8 02/14/05 09:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 7,019 8 02/25/05 01:03 PM
by agar
* test tubes agar fungophiliac 3,711 8 06/19/20 11:41 PM
by gorilliam
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 5,333 12 03/30/20 12:37 PM
by Surf707
* Agar question WormholeSurfer 1,698 3 10/29/05 09:10 PM
by agar
* Agar and the global economy Dem_Bones 1,946 4 03/01/06 11:08 AM
by Dem_Bones
* Agar's Home Made Compost recipe for bulk substrates
( 1 2 3 4 all )		 agar 22,325 75 02/12/21 07:50 AM
by Bart84

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: DERRAYLD, Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
799,862 topic views. 9 members, 183 guests and 103 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.056 seconds spending 0.026 seconds on 18 queries.