catnip40 said:

Before cabin and trench sequestering caught on sandman420 used to talk about a similar idea on discord but just putting the transfer on the wall of a no pour or deeper agar vessel and it would do the same thing the myc would grow down off the wall onto the agar and leave whatever else behind cuz it couldn't grow down the wall

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Kloakk said:

fahtster said:

How I understand cabin sequestering is that bacteria won’t grow through.  It grows on the surface but doesn’t burrow.  The same reason cold pours and trap doors and sandwiches work.  Unless I’m completely misunderstanding the intended use of them.  I don’t think I am though. That’s why I asked if the myc could be pushing the bacteria through with it. 

Technically the transfer inside the cabin is upside down but in my mind, when you make the cuts for the transfer, you’re pushing fragments of whatever was on the surface down to the bottom anyway.  Unless the transfer in the cabin is supposed to be shorter than the thickness of the cabin plate so it doesn’t touch the roof so it grows along the plate bottom and then up the insides of the cabin.  That doesn’t make sense to me tho.. why even have a roof at all then?

If it’s because the meeting of the cover slip and the rest of the plate is supposed to stop bacteria, why wouldn’t the roof itself stop it unless it’s being pushed through.

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Well, the way I understanding it, the myc is supposed to grow between the cabin and the roof, not through the roof. Furter more, glass is not as attractive as agar.

Have you read MycCycle's journal?

https://www.shroomery.org/forums/showflat.php/Number/29049976

I'm just saying, scalloping is one of two things, backy or monokaryon, what do you think your plate is of the two?

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cronicr said:

I'd assume bacteria most molds are pretty good at making themselves be seen eventually, IME spawning scalloped cultures usually just leads to a mess of nothing but I can't recall ever getting any mold from it

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cronicr said:

I'd assume bacteria most molds are pretty good at making themselves be seen eventually, IME spawning scalloped cultures usually just leads to a mess of nothing but I can't recall ever getting any mold from it

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Smellyhobbit said:

Every time I see this technique I feel like it’s different. I thought cabin sequestering was a variation of trenching. I thought you needed a trench around it for it to work.

Or did I just see someone do that? I still have no idea what it’s for.
Seems like a lot of effort to replace regular careful culture cleaning 🤷‍♂️

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Kloakk said:

cronicr said:

I'd assume bacteria most molds are pretty good at making themselves be seen eventually, IME spawning scalloped cultures usually just leads to a mess of nothing but I can't recall ever getting any mold from it

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Agreed, so if it is indeed bacteria, then cabin sequestering is the best way to eliminate it right?

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fahtster said:

I have not seen that.  Appreciate the link.  I was going off of supa’s thread where agar was being used as the roof.  First time hearing of the actual cover slip

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No problem, I just want to do my best to get my FP+ clean

I guess the cabin sequestering came to this community through supa and he is getting it from Paul Grzybowski, now Paul choose to use agar as a roof, but the original technique never mentioned agar, only coverslip.

I do not know if it will make any difference, but what I do know is that I could get high and trip balls to see the meaning of life and ego death any time.. Been and done, I'm not in this for the high, that is just a bonus.. But being part of this community and actually making a difference, well shit, that would make my fuckings day... So, as soon as my T6 FP+ plates grow a little more, I am excited to try the tek and see what it can do..

ThurmanMurman said:

Smellyhobbit said:

Every time I see this technique I feel like it’s different. I thought cabin sequestering was a variation of trenching. I thought you needed a trench around it for it to work.

Or did I just see someone do that? I still have no idea what it’s for.
Seems like a lot of effort to replace regular careful culture cleaning 🤷‍♂️

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I've been trying to figure out how this isn't just trenching with extra steps. Im not saying that's all it is, I'm just saying that I dont get it. I've seen the diagram and whatnot, but I dont understand this "myc growing THROUGH the agar" thing.

Is the transfer supposed to stretch to the walls of the cabin and then grow up and around the roof? That's just trenching with parkour, no?

I also saw on a sequestering write up that the glass slip was a crucial component, and I told myself I'm done buying things that I need for a single application. Wooden pickles aren't selling so well since Christmas is over and I'm fuggin broke.

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cronicr said:

Kloakk said:

cronicr said:

I'd assume bacteria most molds are pretty good at making themselves be seen eventually, IME spawning scalloped cultures usually just leads to a mess of nothing but I can't recall ever getting any mold from it

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Agreed, so if it is indeed bacteria, then cabin sequestering is the best way to eliminate it right?

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Indeed , to find out for sure doing the cabin with 
Antibiotic agar right?

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Yoooo, the both of you, have you seen the seminar? Both your answer is talked about there..

@Thurman, the roof will stop the bacteria because the mycelium will grow between the roof and agar.I just bought 200 slides(that I will cut in half) for 3 dollars. You can afford it.

If you check out Paul's seminar then you will see that antibiotic agar isn't helping anything, it's just producing more antibiotic resistant bacteria..

Here you go boys:

cronicr said:

I got too many beers to drink for that 😆

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You're telling me, man I've been drinking for 10 hours and soon I have to get rid of all the fucking snow outside so I can celebrate my daughter's 7th birthday. Beer is what is giving me the courage to even discuss this with all y'all OG's

But I really want to understanding this, and be better, I don't care how or by who, but be better at cultivation.

Kloakk said:

But I really want to understanding this, and be better, I don't care how or by who, but be better at cultivation.

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cronicr said:

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It's 06:30 in the morning where I'm at.

fahtster said:

Same.  I think it can be a great tool at fighting bac.  It was great for my semp clone

That seminar is what got me excited about it

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Thanks for saying that, to me you are light years beyond me, and FP+ is my nr.1 goal to clean, and knowing we have the same goal is really good. I know, I am John Snow and I know nothing, but I really want to know