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Re: AGAR ENVY! (Anything and All things agar!) [Re: Cak Le Boof] * 1
    #29114895 -

I am very new to growing mushrooms, and I have a million questions but it seems my next step is agar.

I am planning to follow BOD's agar TEK and am wondering if this is still best practice?

Also how thick (deep) do you pour your plates for isolation?


--------------------
Knowledge at all cost.  That which can be destroyed by truth should never be spared its demise.

Love is my reason and I will never need another.

Disobedience is the true foundation of liberty. The Obedient must be slaves.
-Thoreau

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mara] * 3
    #29114969 -

Mara said:
Also how thick (deep) do you pour your plates for isolation?



I just pour enough to cover the surface of the bottom of the plate.  I am not too worried about thickness.

Edited by golfin_ernie (02/05/25 04:52 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mara]
    #29115091 -

Mara said:
I am very new to growing mushrooms, and I have a million questions but it seems my next step is agar.

I am planning to follow BOD's agar TEK and am wondering if this is still best practice?

Also how thick (deep) do you pour your plates for isolation?



Welcome.

I pour plates about 1/8 inch, maybe a little more. It can be from that to a quarter inch and still be fine. I do a quarter inch when I'm storing plates with mycelium in the fridge long term. Sometimes I'll pour plates 1/8 inch thick but pour the last few 1/4 inch to use up the agar I have.

It barely matters since most of what you're doing with agar is basically 2D.

I'd skip Bod's 9 year old tek, it's got the old wrong "sectors" b.s.
If do that you'll spend hours chasing something you don't need to.

7 years ago Bod even said not to bother with sectors:
https://www.shroomery.org/forums/showflat.php/Number/24681493#24681493

I'd do this for pouring plates:
https://www.shroomery.org/forums/showflat.php/Number/27551422#27551422

And this for transfers:
https://www.shroomery.org/forums/showflat.php/Number/29095187

Edited by nektar61 (02/05/25 07:20 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mara] * 1
    #29115120 -

Mara said:
I am very new to growing mushrooms, and I have a million questions but it seems my next step is agar.

I am planning to follow BOD's agar TEK and am wondering if this is still best practice?

Also how thick (deep) do you pour your plates for isolation?



It's a great Tek and bod outlines over and over that worrying about sectors isn't worth it in that 9 year old Tek so don't worry about it follow bods Tek and you'll see success


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 4
    #29115129 -

nektar61 said:
Mara said:
I am very new to growing mushrooms, and I have a million questions but it seems my next step is agar.

I am planning to follow BOD's agar TEK and am wondering if this is still best practice?

Also how thick (deep) do you pour your plates for isolation?



Welcome.

I pour plates about 1/8 inch, maybe a little more. It can be from that to a quarter inch and still be fine. I do a quarter inch when I'm storing plates with mycelium in the fridge long term. Sometimes I'll pour plates 1/8 inch thick but pour the last few 1/4 inch to use up the agar I have.

It barely matters since most of what you're doing with agar is basically 2D.

I'd skip Bod's 9 year old tek, it's got the old wrong "sectors" b.s.
If do that you'll spend hours chasing something you don't need to.

7 years ago Bod even said not to bother with sectors:
https://www.shroomery.org/forums/showflat.php/Number/24681493#24681493

I'd do this for pouring plates:
https://www.shroomery.org/forums/showflat.php/Number/27551422#27551422

And this for transfers:
https://www.shroomery.org/forums/showflat.php/Number/29095187



Have you even read bods Tek or you just running around telling people that the infos bad for no reason 😂 maybe you should edit his too


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr] * 6
    #29115132 -

Many people get overly concerned with finding an isolate. Many isolates suck.
any two spores that successfully mate make a strain.

thus with spores on a petri dish


so you see a clone has proven it's potential by growing, with isolates your going blind you need to test every isolate, some will suck
here's a good example

Also a lot of noobs worry about rhizomorphic growth. Worry about healthy growth. you do not know if rhizomorphic growth will be any good till you test it. so tomentose growth may be a better fruiter or more potent, you won't know so don't concentrate on only rhizomorphic mycelium on dishes

That's literally everything he says about strain isolation maybe try show some respect out here before running around flinging shit


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr]
    #29115241 -

cronicr said:
....maybe try show some respect out here before running around flinging shit



You seem to be the ONLY person getting upset at me trying to get new growers off of wasting time on this ancient voodoo that most great growers don't do anymore.

Other people thank me for saving them time.

You actually had the audacity to say that I "shit on those whose shoulders we stood on to get here" because I edited and improved an old tek (from a guy who hasn't been here in years, and I credited him, per the Creative Common license that covers this whole site. The bottom of every page here says "Some rights reserved" and links that license, which allows "remixing", with credit.) Ythan put that here, and it sums up the spirit of this site.

FWIW, Beef already re-linked my edit of that old tek in his "Hitchiker's Guide to the Shroomery", so he's not sending new growers down a dead-end rabbit hole like you seem to want to do.

SmellyHobbit and I are working on a new improved tek. It's over half written, but will take a while to grow out plates to take custom photos for it.

Yo'ure acting childish. And a long-time very respected member here already said "fuckin cron. so dramatic hahah" about it.

I don't know what your need is to constantly shame me on this, on at least 3 threads now, but it's unseemly. 

I'm saving new growers time. You're obsessed with me. Move on, man.

Edited by nektar61 (02/05/25 10:00 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 3
    #29115251 -

No I'm not obsessed I just think your lazy and and coming at it with a half assed approach, you're talking about bods info when his whole fucking section is stating what we've all been saying for fucking years get over yourself it's nothing groundbreaking and who are you to try call out these people with zero proof of your own you are bringing nothing to the table here.
It would be easy to respect you writing a Tek and saying exactly what you're saying now but when you copy and paste somebody else's work and go into.their thread and say it's bad info that's shitty fucking etiquette with zero proof, expecially proof of your own you're putting zero work in but sit here being a keyboard warrior and want people to jump on board and I just showed u bod said nothing about sectors being the way to go in the very Tek u said had bad fucking info, grow up.


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Edited by cronicr (02/05/25 10:13 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr] * 3
    #29115254 -

So I'll say it again...read bods Tek especially the section of isolates and try to tell us how he has bad info there ....also show your work


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr] * 3
    #29115256 -

Many people get overly concerned with finding an isolate. Many isolates suck.
any two spores that successfully mate make a strain.

thus with spores on a petri dish

so you see a clone has proven it's potential by growing, with isolates your going blind you need to test every isolate, some will suck
here's a good example

Also a lot of noobs worry about rhizomorphic growth. Worry about healthy growth. you do not know if rhizomorphic growth will be any good till you test it. so tomentose growth may be a better fruiter or more potent, you won't know so don't concentrate on only rhizomorphic mycelium on dishes





There ya go because I know how lazy you are that's everything bod said in that section ....so tell us again where he's wrong


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr] * 2
    #29115259 -

whats with the tone cronic?  shes trying to help.  thats a wild way to come slanted at a long-standing member.  what if everyone took a step back here and refocused our comitment: were trying to help people get where they need to be.  maybe lets lighten up and recalibrate?


--------------------
Need a lift?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #29115261 -

nektar61 said:
FWIW, Beef already re-linked my edit of that old tek in his "Hitchiker's Guide to the Shroomery", so he's not sending new growers down a dead-end rabbit hole like you seem to want to do.




You'll never see me or beef or bod or faht or Stipe or Darryl or a.k.a. or 3.a.m. or smelly or any of us ever suggest you chase down isolates don't clown yourself and don't put words in my mouth when tf you ever see anybody suggest that route , even in stro's Tek he doesn't suggest it same with bods that you just linked , stop being disrespectful about it and I would simply sit here and agree with you but when you come at it from an angle like this I'm not gonna sit back and watch.


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #29115263 -

BeefSupremeJr said:
whats with the tone cronic?  shes trying to help.  thats a wild way to come slanted at a long-standing member.  what if everyone took a step back here and refocused our comitment: were trying to help people get where they need to be.  maybe lets lighten up and recalibrate?



Wise words.

Thanks. I'm not ignoring his questions but I'm not answering him anymore, he gets mad no matter what.

I should have told the new person asking "Bod's tek is fine, but I'd ignore the parts about sectors, it's not needed." And I will continue to offer that advice when it comes up.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme] * 2
    #29115264 -

BeefSupremeJr said:
whats with the tone cronic?  shes trying to help.  thats a wild way to come slanted at a long-standing member.  what if everyone took a step back here and refocused our comitment: were trying to help people get where they need to be.  maybe lets lighten up and recalibrate?



You gonna sit here and talk to me about coming at long standing members 😂😆 that's my whole fucking point bud
It would be easy to say you don't need to do x or z without trying to drag bod and stro or my name through the mud and we've been here alot fucking longer than her


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 3
    #29115265 -

nektar61 said:
BeefSupremeJr said:
whats with the tone cronic?  shes trying to help.  thats a wild way to come slanted at a long-standing member.  what if everyone took a step back here and refocused our comitment: were trying to help people get where they need to be.  maybe lets lighten up and recalibrate?



Wise words.

Thanks. I'm not ignoring his questions but I'm not answering him anymore, he gets mad no matter what.

I should have told the new person asking "Bod's tek is fine but I'd ignore the parts about sectors it's not needed." And I will continue to offer that advice when it comes up.



Bods advise in the thread is sectors aren't needed 😆


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr] * 2
    #29115268 -

cronic i can see youre upset. were all in the same timezone.  what say we let it idle for a night? were all tired.  i respect you but I doubt anything productive will be acheived with this kindof energy.


--------------------
Need a lift?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #29115269 -

:fine:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme] * 2
    #29115270 -

Lets sort this out later.  Its not as though any us are being paid.  a rest is in order.  were all bigger than this.


--------------------
Need a lift?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #29115273 -

But seriously people don't chase isolates by the time you get to t8 you could be on 5 t3's of a clone set from t2 of your ms plate don't be stupid


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr]
    #29115277 -

Im sure we can sort it out without calling vet members stupid cron. ouch.


--------------------
Need a lift?

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