BeefSupremeJr said:

yeah.  i agree we need a new tek guys.

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BeefSupremeJr said:

yeah.  i agree we need a new tek guys.

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SporeHopper said:

I read about isolating strains in Stro's Cleaning and Isolating On Agar which were linked to in Hitchhikers Guide to Shroomery. I just assumed it was still relevant.

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B Traven said:

... which is compulsively cloning plins until you're drowning in a bunch of closely related cultures that you can't bear to part with.

Ok, maybe that's just me...

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B Traven said:

I also concur that chasing sectors and monocultures is an outdated and unrealistic objective.

Things should be updated to the new state-of-the-art, which is compulsively cloning plins until you're drowning in a bunch of closely related cultures that you can't bear to part with.

Ok, maybe that's just me...

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sage_bd said:

Why do you prefer plins vs actual fruit from a dense pin set in tubs?

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Phaethon said:

Nothing to worry about there, IMO. The section at 12 o'clock just is just less rhizomorphic, and the discoloration is most likely bruising (from drying out or a temp swing - which also can dry out an agar plate).

Just make a new plate from growth on that plate that looks healthy and organized, which is most of it, especially in the lower half.

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Thanks a lot, that's good to know!

What would make the area at 12 0clock look like a void? I'm new and trying to get a handle on the differences.

Cak Le Boof said:

Phaethon said:

Nothing to worry about there, IMO. The section at 12 o'clock just is just less rhizomorphic, and the discoloration is most likely bruising (from drying out or a temp swing - which also can dry out an agar plate).

Just make a new plate from growth on that plate that looks healthy and organized, which is most of it, especially in the lower half.

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Thanks a lot, that's good to know!

What would make the area at 12 0clock look like a void? I'm new and trying to get a handle on the differences.

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I'm not really sure. Could be a section of actually different genetics, it might be fighting some bacteria there, or it might be just random expression. I've had plates throw weird sectors over multiple transfers.

Phaethon said:

Cak Le Boof said:

Phaethon said:

Nothing to worry about there, IMO. The section at 12 o'clock just is just less rhizomorphic, and the discoloration is most likely bruising (from drying out or a temp swing - which also can dry out an agar plate).

Just make a new plate from growth on that plate that looks healthy and organized, which is most of it, especially in the lower half.

---

Thanks a lot, that's good to know!

What would make the area at 12 0clock look like a void? I'm new and trying to get a handle on the differences.

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I'm not really sure. Could be a section of actually different genetics, it might be fighting some bacteria there, or it might be just random expression. I've had plates throw weird sectors over multiple transfers.

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Scemilope said:

Finally, an excuse to cult today!

tamp(ATL7)

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A couple plates from these pins didn't make the cut but this lil guy did pretty good.

Smellyhobbit said:

Gymnopilus luteofolius F1 streak. Very fresh spores. Wicked fast germination.

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Cak Le Boof said:

Phaethon said:

Cak Le Boof said:

Phaethon said:

Nothing to worry about there, IMO. The section at 12 o'clock just is just less rhizomorphic, and the discoloration is most likely bruising (from drying out or a temp swing - which also can dry out an agar plate).

Just make a new plate from growth on that plate that looks healthy and organized, which is most of it, especially in the lower half.

---

Thanks a lot, that's good to know!

What would make the area at 12 0clock look like a void? I'm new and trying to get a handle on the differences.

---

I'm not really sure. Could be a section of actually different genetics, it might be fighting some bacteria there, or it might be just random expression. I've had plates throw weird sectors over multiple transfers.

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Interesting, thanks. I'm finding a lot of my transfers from areas of strong, visually similar rhizome growth grow out to look similar to your example.

Do you put plates like your example to grain or aim to get myc that is similar looking all the way around first? I imagine both have their advantages.

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I send them to grain. As long as all the growth looks healthy I'm not worried too much about the sectoring. I'm sure there are differing opinions here.

Phaethon said:

Nothing to worry about there, IMO. The section at 12 o'clock just is just less rhizomorphic, and the discoloration is most likely bruising (from drying out or a temp swing - which also can dry out an agar plate).

Just make a new plate from growth on that plate that looks healthy and organized, which is most of it, especially in the lower half.

---

Thanks a lot, that's good to know!

What would make the area at 12 0clock look like a void? I'm new and trying to get a handle on the differences.

---

I'm not really sure. Could be a section of actually different genetics, it might be fighting some bacteria there, or it might be just random expression. I've had plates throw weird sectors over multiple transfers.

---

Interesting, thanks. I'm finding a lot of my transfers from areas of strong, visually similar rhizome growth grow out to look similar to your example.

Do you put plates like your example to grain or aim to get myc that is similar looking all the way around first? I imagine both have their advantages.

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I send them to grain. As long as all the growth looks healthy I'm not worried too much about the sectoring. I'm sure there are differing opinions here.

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Great, thanks. I'd rate you if I had enough posts to do so! The mixed genetics can be a strength from what I understand, especially if looking to clone from whatever comes out strongest.