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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kohler] * 3
    #28360659 -

The HBO Mak swab had a 66% success rate! Granted, 1 is moving significantly slower than the other but 2/3 germ rate is pretty darn good for a shit grower like me I feel like!

The KSSS bacterial pin clone sample ended up outperforming the bacteria and quite literally swallowed it and I’ve now got plenty of usable material for a clean transfer! So stoked I’ll be able to save a failed grow considering I pumped 10 cc’s of spores into an AIO bag in all of my infinite noob wisdom. At least I don’t have to buy more to try again.



--------------------
Exploring the Myceliverse sharing Spore Stories

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kohler] * 1
    #28360664 -

Kohler said:
Hey guys, can I get a quick evaluation on these two plates?



I'd transfer from the right outter edge of this one. Looks better than the other plate. Left side of this plate has some small dots at 8 and 11 that might be bacteria so I'd transfer from 2 to 5 oclock and go to several new plates.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 5
    #28360703 -

Found a few neat cultures from my AMVPxTBC swabs :dancer:  So far all the test tubs have had both normal fruit initially and then fin clusters start showing up about the time you're harvesting the first flush of normal fruits.




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Re: AGAR ENVY! (Anything and All things agar!) [Re: PBJ710]
    #28360713 -

Hey everybody, just wanted to post a dish and ask if this is plate pins happening and then I see the post above.

I guess it's clear it's plins, but what do I do with this.

I left the plate to throw it out since it looked sketchy. Now this.
Can I give it some water or is that a nono?
If yes how?
What do I do with the plins? Do I put it to grain or can I experiment with these in some other way?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #28360718 -

nektar61 said:
Kohler said:
Hey guys, can I get a quick evaluation on these two plates?



I'd transfer from the right outter edge of this one. Looks better than the other plate. Left side of this plate has some small dots at 8 and 11 that might be bacteria so I'd transfer from 2 to 5 oclock and go to several new plates.


Thanks for your response!

I think these are just specks on the outside of the plastic, but it's just about the best time to transfer anyways, so I will go ahead with that.

Other than that, do you feel somewhat confident that this is indeed cube and not just random mold contam? :smile:

Edited by Kohler (06/15/23 11:42 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 2
    #28360743 -

Smellyhobbit said:
Just a cool plate




I know what that is and won't tell.
Project X-5.
Secret.
I have it too, somehow.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28360751 -

That's an amazing plate! If my plates looked half that good I would be very happy 😊


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket]
    #28361238 -

Would using 1.6-1.8% nutrients help with cleaning up germ plates


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket]
    #28361241 -

I am considering using the low nutrient agar for taking transfers from a germ plate or from a plate with some contamination. I know I should take my transfer far from Amy obvious contamination just seeking an expert opinion on low nutrient agar. I've been battling some contaminated jars and want to get the cleanest mycelium possible to eliminate it as a source of contamination. I will also be PCing my jars for a full 2 hours from now on.


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kohler]
    #28361289 -

Kohler said:
nektar61 said:
Kohler said:
Hey guys, can I get a quick evaluation on these two plates?



I'd transfer from the right outter edge of this one. Looks better than the other plate. Left side of this plate has some small dots at 8 and 11 that might be bacteria so I'd transfer from 2 to 5 oclock and go to several new plates.


Thanks for your response!

I think these are just specks on the outside of the plastic, but it's just about the best time to transfer anyways, so I will go ahead with that.

Other than that, do you feel somewhat confident that this is indeed cube and not just random mold contam? :smile:


The 1-inch white circle in the middle? If you noced this plate with cubes, that is cubes.

I can't guarantee 100% there are zero contams in there, since some can be invisible with naked eye. But it looks right and I'd transfer it.

If this plate didn't have those possibly bacterial dots that you think are plate imperfections, of if you are 100% sure they are on the outside of the plastic, not inside, I wouldn't rule out sending a doughnut of the outside half-inch of the white circle to grain.

But if those dots are inside, I'd do one more transfer. And, you know, even if the dots are on the outside, if you want your plates to look like a SmellyHobbit plate.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 2
    #28361793 -


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Chamo]
    #28361924 -

I just poured a few agar cups and I evidently cut some corners that made my cups a little different. I used barley malt extract and there’s a lot of particles in it. How would I filter those on the next batch?  Would a coffee filter work after it’s been heated and mixed?

I put some spores to the cups I made and I see a lot of growth (not contams, out of 25 I had 4 that had mold and one with bacteria, I kept the bacteria since I’ll transfer that soon but the mold ones I just tossed). Do you all typically try to get every growth separated to make sure there’s no contams we can’t see or do you just send it to grain if there’s nothing showing just to see which ones fruit?  I sent some mss plates to grains but they’re cubes and the next batch I have other species so I was thinking of sectoring until I get clean growth. Only thing with that is I made like 3-4 cups with each strain and it’s looking like 10+ colonies on each so I could end up with a LOT OF CUPS lol.

I’m also not sure if the growth in seeing is growth from one spore or if they’ve mated, am I being ridiculous thinking that the growth I see might be monokaryotic?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Chamo]
    #28361937 -

Chamo said:



Beautiful plates


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A Handy Dandy Rule Book for the Beginner Cultivator /WBS flash prep Tek /Bulk Substrate Coir Tek /Shoebox Tek /Bottle Tek /Clean spawn Checklist /Agar Recipe /Don't put Spores to Grain /Updated Pf Tek /Surface Conditions


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pandaskis]
    #28362114 -

Thanks man. I’ve been working on a different project for a few months. I just cloned these last week and the plates I got were so interesting I think I’m going to put the older project on pause and see how these do.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28362125 -

Are we supposed to be doing transfers this early?
I’ve been painstakingly waiting for my plates to more or less fill out before I even think about touching them.


--------------------
Exploring the Myceliverse sharing Spore Stories

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TonyCanoli] * 3
    #28362149 -

Those are more than 50% full, contam or weird shit would have showed up by now.

Also fuck me, I did like 10 T3 transfers and they all seem to be less organized than the mother plates. :justfuck:


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TonyCanoli] * 1
    #28362341 -

TonyCanoli said:
Are we supposed to be doing transfers this early?
I’ve been painstakingly waiting for my plates to more or less fill out before I even think about touching them.


Many people would let that grow out half again as big, but most people don't let it grow all the way to the edge to transfer.

Point of transferring at this point is to get clean mycelium, and to get good looking, well growing mycelium. You can do both at that point you show there, but some people wait. I don't see the need to. You've got organized rhizo-y looking mycelium on the edge, and it looks clean. Waitging longer lets any hidden contam grow out.

I let it grow out more on the T2 or whatever I'm sending to grain, because I want more mycelium to send to grain. I usually do 7 quart jars at once, because that's what fits in my PC.

But if you're looking for transfers, I don't see the reason to wait.

I would do it now, but nothing will be harmed by letting it grow 2 or 3 more days (depending how fast it's growing.) 

Anyone who replies contradicting all this, I believe is showing
a personal preference, not science, and should "show their work" and say why they let it grow out more before a transfer.

Growing all the way to the edge can lead to contam too, if you're plate isn't entirely sealed.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (06/16/23 04:41 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TonyCanoli]
    #28362347 -

TonyCanoli said:
Are we supposed to be doing transfers this early?
I’ve been painstakingly waiting for my plates to more or less fill out before I even think about touching them.


I posted a long reply explaining why I would be fine with transferring now. But my post is gone. I'm showing on Threads page as last person to post on this thread too. But page here is showing someone else as last post.

Did I accidental block myself? hahah.

Shroomery Database is drunk. I'll let Ythan know.

EDIT: now it's showing, above this post.

Glad I'm not tripping.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (06/16/23 04:48 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28362367 -

Normally I make transfers at about the size of a quarter. I am a little behind. But no harm done.




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Re: AGAR ENVY! (Anything and All things agar!) [Re: Chamo] * 3
    #28362408 -


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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