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Amazon Shop for: Agar, Petri Dish

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Anonymous

Agar
    #2558792 - 04/14/04 12:49 PM (12 years, 9 months ago)

i know this is a stupid question but is there anyway to work with agar without a pc, mine is broken and i do not want to buy a new one unless it is absolutly nessecary.


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Invisiblemuse_sick
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Re: Agar [Re: ]
    #2558997 - 04/14/04 01:35 PM (12 years, 9 months ago)

no
edit to say:you can buy prepoured sterile agar online from some of the vendors if i'm not mistaken


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Edited by musesicksic (04/14/04 01:36 PM)


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Invisiblemuse_sick
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Re: Agar [Re: muse_sick]
    #2559007 - 04/14/04 01:37 PM (12 years, 9 months ago)



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Re: Agar [Re: ]
    #2559163 - 04/14/04 02:15 PM (12 years, 9 months ago)

put your agar ingredients in your petri dishes, and stick them on a cookie sheet. preheat your oven to 250degrees and put them in for 45mins-1 hour after the oven reached 250... let cool and solidify before germinating... if you have plastic petri dishes your a moron. if they arent tempered... your a moron.
pyrex petri dishes work best...


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InvisiblePrisoner#1M
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Re: Agar [Re: ThatDaltonGuy]
    #2560257 - 04/14/04 06:14 PM (12 years, 9 months ago)

Quote:

ThatDaltonGuy said:
if you have plastic petri dishes your a moron. if they arent tempered... your a moron. pyrex petri dishes work best...




Pyrex and kimax are best because they are borosilicate... Polymethylpentene is a polycarbonate thats autoclavable... they rock... soda lime glass works pretty damn good


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InvisibleLoki
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Re: Agar [Re: ]
    #2560279 - 04/14/04 06:19 PM (12 years, 9 months ago)

I hardly ever pc agar, I only pc it for spore work.
If cloning and just doing transphers, I add h2o2 instead of
pcing, but you need to add it as the agar cools from
boiling, I'll try to find the tec i follow and link you


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InvisibleLoki
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Re: Agar [Re: ]
    #2560284 - 04/14/04 06:21 PM (12 years, 9 months ago)



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Anonymous

Re: Agar [Re: Loki]
    #2562604 - 04/15/04 09:15 AM (12 years, 9 months ago)

thanks man, but i will be using it for spore work, any advice? i am using a wild print so i'm not exactly sure how it will go, and advice on working with wild prints?


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InvisibleBi0TeK
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Re: Agar [Re: ]
    #2563057 - 04/15/04 12:15 PM (12 years, 9 months ago)

Pasturize a small cardboard square in the microwave for several minutes and transfer it to peroxidated agar, then transfer some spores on to the cardboard square using a sterilized scalpel. The cardboard acts as a barrier to protect the spores from the H202.

This method works great for both wood lovers and P cubensis.  :thumbup:


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Amazon Shop for: Agar, Petri Dish

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