With Aspergillus it is best to perform mycelium transfer to a new plate at the first sign of a problem. Otherwise it can spread all over the plate before you know it. Remember you can't see that it has spread till it is too late. This is one of the first contams I ran into too...very common on MEA an grains. Oh, one other thing...keep the amount of mycelium you transfer to a minimum...very small piece...as opposed to the standard size wedge.

And not that you will need to do this...but if you have only a contaminated sample of a hard to replace strain or wild strain you could at this point...take another agar plate and remove the agar and lay the new agar on top of the contaminated agar/mycelium...then cut a couple of dime size holes clear thru both agar layers...remove the circle of agar. Aspergillus although it travels thru the air readily, advances across agar at a much slower rate than cubensis mycelium. By doing this you restrict the spread of the Aspergillus to it's slowest mode of transport (removing the air borne vector). The mycelium will grow up and out the holes and on ot the top of the upper agar surface. This mycelium will be clean. Then you could perform your transfer. But remember that this will only be a mycelium transfer as opposed to an mycelium/agar wedge transfer. The bottom of the upper layer of agar could very well be contaminated. One last note. The holes you cut should be located over the existing mycelium such that the hole intersects the mycelium. This is a method I use for isolating strains from the field...you may find applications for it in your own adventures in the future. Good Luck

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Peace, Love & Light

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Peace, Love & Light