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Anonymous

Re: mold in agar plate
    #67418 - 12/03/99 10:39 AM (22 years, 10 months ago)

What you are describing is Aspergillus (a.k.a, Green Mold). Although it grows on the agar's surface, its main mode of transport is thru the air. So, jarring your plates can cause it to spread.

With Aspergillus it is best to perform mycelium transfer to a new plate at the first sign of a problem. Otherwise it can spread all over the plate before you know it. Remember you can't see that it has spread till it is too late. This is one of the first contams I ran into too...very common on MEA an grains. Oh, one other thing...keep the amount of mycelium you transfer to a minimum...very small piece...as opposed to the standard size wedge.

And not that you will need to do this...but if you have only a contaminated sample of a hard to replace strain or wild strain you could at this point...take another agar plate and remove the agar and lay the new agar on top of the contaminated agar/mycelium...then cut a couple of dime size holes clear thru both agar layers...remove the circle of agar. Aspergillus although it travels thru the air readily, advances across agar at a much slower rate than cubensis mycelium. By doing this you restrict the spread of the Aspergillus to it's slowest mode of transport (removing the air borne vector). The mycelium will grow up and out the holes and on ot the top of the upper agar surface. This mycelium will be clean. Then you could perform your transfer. But remember that this will only be a mycelium transfer as opposed to an mycelium/agar wedge transfer. The bottom of the upper layer of agar could very well be contaminated. One last note. The holes you cut should be located over the existing mycelium such that the hole intersects the mycelium. This is a method I use for isolating strains from the field...you may find applications for it in your own adventures in the future. Good Luck :wink:

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Peace, Love & Light



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OfflinePlacebo
member
Registered: 12/16/98
Posts: 55
Last seen: 21 years, 2 months
Re: mold in agar plate
    #67419 - 12/03/99 07:17 PM (22 years, 10 months ago)

If you get some mycelial growth and the mold hasn't grown on to the surface of the mycelium, then there's a good chance that you'll be able to isolate the mycelium by doing a transfer. Just be careful that you only transfer mycelium and that there isn't any mold on the piece that you cut out.


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Invisiblerebelmoon
enthusiast
Registered: 04/05/00
Posts: 204
Re: mold in agar plate * 1
    #67421 - 12/03/99 12:04 PM (22 years, 10 months ago)

thanks for the advice! i have a bunch of agar plates growing right now and this appears to be the only conaminated one. since i have other plates and the mycelium *has not started to grow* yet on the green mold contaminated plate, im thinking i will just scrap it. but just for future reference, will an agar plate grow mycelium even with a developed circle of green mold in it? thanks.
-daniel.


--------------------
"habit is the ballast that chains the dog to its vomit" - s beckett


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Anonymous

Re: mold in agar plate
    #67422 - 12/04/99 09:35 AM (22 years, 10 months ago)

well...yes, and the mycelium can even cover the contamination too...but you don't want to keep mycelim in the same plate with contamination. You increase the chance of contamination to be transfered with your mycelium/agar wedge to the grain jar. If you have that many good plates...toss the bitch.

------------------
Peace, Love & Light



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