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OfflinePsilowarrior
enthusiast
Registered: 11/20/99
Posts: 195
Last seen: 22 years, 10 months
Re: agar plate question
    #67810 - 12/07/99 12:18 PM (24 years, 4 months ago)

The first time I tried agar culture I pressure cooked for 45min at 15psi and the malt extract did carmelize. 15min at 15psi is more appropriate. But if you've got a plate full of mycellium the media is likely not your problem. I'd cut a piece of your plate out and drop it on another plate (like the picture) and you will likely get strandy growth.

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Anonymous

Re: agar plate question [Re: Psilowarrior]
    #67812 - 12/07/99 01:05 PM (24 years, 4 months ago)

What strain are you growing? Cubensis? What plate number are you on? The whole idea of using agar is to grow out the mycelium and select the best. Often you will not see the really nice ropey growth till the second plate...depends on the substrain of cubensis your growing. I've noted that often when you use a few grams of substrate in the agar...the ropey growth is delayed a little. I prefer to use the powdered substrate in the agar...it will benifit you greatly during the colonization phase.

------------------
Peace, Love & Light


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Invisiblerebelmoon
enthusiast
Registered: 04/04/00
Posts: 204
Re: agar plate question [Re: Psilowarrior]
    #67813 - 12/10/99 02:09 PM (24 years, 4 months ago)

thanks for the replies! it makes me feel all warm and cuddly to know that people care.

shroom mage: i am growing b+ cubensis from workman. and this is my first plate. i will do a transfer this weekend. i imagine the agar media from shroomgod had the wheat flour powdered, since it comes as a flour. is this what you mean? thanks for the reassurance shroom mage. ill let you know how things are going on my second plate. in your experience, how many transfers does the b+ substrain require?
-daniel.

[This message has been edited by rebelmoon (edited December 11, 1999).]



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"habit is the ballast that chains the dog to its vomit" - s beckett

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