ShroomGod

maybe throu the temp difference the agarcontainers sucked air in (light vacuum)
our fridge with greenmoldcheese easyly would spoil all cultures....

BiO.

Now if your agar cultures do not have this green mold growing on them then you can eliminate the posibillity that your agar cultures are the source of the contamination. You can also eliminate the possibility that your transfer tool is introducing contamination because if it were you would also contaminate the agar.

You may want to try again and have a couple of jars of substrate serve as a negative controls. One of these jars you don't even open and the other you open and pretend to drop some agar into it. Depending on what hapens you can determine if you are introducing the contaminant or if your substrate is simply not sterile.

I had the same problem with some old stored Psilocybe cyanescens plates. Nearly every one grew mold when transfered to fresh agar or grain, even though they looked clean. Time to start again with fresh plates. I now store my plates wrapped tightly with plastic wrap and then placed inside a ziplock bag before refrigeration.

Everytime I have stored petri dishes in the frig. and then tried to use them I go contamination...every single time. So I just stopped doing it...I have had success ever sense.

The Keeper

I think I'll take Psilowarrior's advice and try to expand an tiny piece of mycelium from one of these contaminated agar plates out on clean plates. If it grows clean, then the chances of contamination from this new agar plate are extremely low. Then, using Workman's advice, I'll store these grown-out jars in airtight trashbags with ties. The jars shouldn't contaminate since the interior of the trashbag will be relatively clean compared to my refrigerator, and just to make sure, I'll spray the inside of the bag with lysol.

ShroomGod

Good Luck
And may the shroom
guide and teach you well

Wild Strain