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Agar
    #2558792 -

i know this is a stupid question but is there anyway to work with agar without a pc, mine is broken and i do not want to buy a new one unless it is absolutly nessecary.

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Re: Agar [Re: ]
    #2558997 -

no
edit to say:you can buy prepoured sterile agar online from some of the vendors if i'm not mistaken


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:beakedwhale:

Edited by musesicksic (04/14/04 11:36 AM)

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Re: Agar [Re: muse_sick]
    #2559007 -


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Re: Agar [Re: ]
    #2559163 -

put your agar ingredients in your petri dishes, and stick them on a cookie sheet. preheat your oven to 250degrees and put them in for 45mins-1 hour after the oven reached 250... let cool and solidify before germinating... if you have plastic petri dishes your a moron. if they arent tempered... your a moron.
pyrex petri dishes work best...


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Let me remind you that the above text is completely false and has been typed to you by an escaped mental patient and shouldn't be taken seriously unless you yourself also have a mental condition. These kind of things need to be taken into consideration by those viewing these threads.

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Re: Agar [Re: ThatDaltonGuy]
    #2560257 -

ThatDaltonGuy said:
if you have plastic petri dishes your a moron. if they arent tempered... your a moron. pyrex petri dishes work best...



Pyrex and kimax are best because they are borosilicate... Polymethylpentene is a polycarbonate thats autoclavable... they rock... soda lime glass works pretty damn good

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Re: Agar [Re: ]
    #2560279 -

I hardly ever pc agar, I only pc it for spore work.
If cloning and just doing transphers, I add h2o2 instead of
pcing, but you need to add it as the agar cools from
boiling, I'll try to find the tec i follow and link you

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Re: Agar [Re: ]
    #2560284 -


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Re: Agar [Re: Loki]
    #2562604 -

thanks man, but i will be using it for spore work, any advice? i am using a wild print so i'm not exactly sure how it will go, and advice on working with wild prints?

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Re: Agar [Re: ]
    #2563057 -

Pasturize a small cardboard square in the microwave for several minutes and transfer it to peroxidated agar, then transfer some spores on to the cardboard square using a sterilized scalpel. The cardboard acts as a barrier to protect the spores from the H202.

This method works great for both wood lovers and P cubensis.  :thumbup:


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PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.

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