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InvisibleADoobie
AdoobieShroomery
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G2G transfers...an endless cycle?
    #3490471 - 12/13/04 12:07 PM (12 years, 6 months ago)

I was wondering...since myc is a mold and mold spreads, could one theoreticly just use a sample of myc tissue from fruiting substrate to inoculate compost/grain/straw? If so, is there a limit to this cycle?

Thanks...


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ADoobie said:
I know what strikes me as odd about this. It is totally unnatural. The whole idea is just totally backwards.

So are historians going to look back at 'The Great Cake Eaters'? Damn. That was a backwards step in the evolutionary process. We left Shroomheads in the dust. Welcome to the new age of Vermheads.

VvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVv

I ONLY STUDY legal, edible mushrooms and mycology. Any mention of any other kind of mushroom is purely fictional, and pictures are ripped from the internet. I do not grow mushrooms!


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
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Re: G2G transfers...an endless cycle? [Re: ADoobie]
    #3490492 - 12/13/04 12:16 PM (12 years, 6 months ago)

Yes there is a limit to how many times one should perform a g2g culture . It is said and practiced that one should not go beyond a third generation of g2g . The reason is the genetics of the mush starts to degrade . But depending on how many jars , bags , etc one starts with , an unlimited supply could be exponetially produced . 1 Jar can turn into 4 , those 4 can become 16 and so on and so fourth . But eventually one must go back to the start to keep the genetics in control .
Hope this helps
Good Luck
Trip n D


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Invisibleohmatic
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Re: G2G transfers...an endless cycle? [Re: tripndicular]
    #3490873 - 12/13/04 01:53 PM (12 years, 6 months ago)

actually, there is, as far as i know, degenereation effects starting from generation 3 onwards.
peace ohm :mushroom2:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Invisibletripndicular
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Re: G2G transfers...an endless cycle? [Re: ohmatic]
    #3490896 - 12/13/04 01:58 PM (12 years, 6 months ago)

I think that is more or less what I said .
"one should not go beyond 3 gens "

Resistance is futile unless you are an ohm !


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Offlinespliffmasta
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Re: G2G transfers...an endless cycle? [Re: tripndicular]
    #3491304 - 12/13/04 03:08 PM (12 years, 6 months ago)

Why are they called generations? Doesn't the word generation more appropriately describe the offspring of the mushroom (cloning the tissue of a mushroom that was propogated via a clone)?

The myc is just growing and spreading during G2G. How does one define along the myc's growth process what is a generation? Is there a volumetric quantity that is typical?

Say you get a 10 gallon master jar. Then you G2G 10% to a half pint jar. Then this half pint jar 10% to another one, and so on. Weren't there more generations and cell replications in the single 10 gallon culture than there were in the subsequent number of G2G's following (permitted it didn't go beyond 10 gal)?

Or does a 100% colonized substrate like complete its growth somehow and reopens for cloning which is any further growth once the myc network reaches 100% and finishes? Seems farfetched, I just don't understand all of this.


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InvisibleADoobie
AdoobieShroomery
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Re: G2G transfers...an endless cycle? [Re: spliffmasta]
    #3491416 - 12/13/04 03:29 PM (12 years, 6 months ago)

Good thoughts spliff...

Now to add to it, what if you didn't wait for 100% colonization, but just kept it's expansion process going by subtracting culter and possibly adding fresh sterilized substrate? This way your 10gal container never looses more than like %25 of it's original "generation"...?


--------------------
ADoobie said:
I know what strikes me as odd about this. It is totally unnatural. The whole idea is just totally backwards.

So are historians going to look back at 'The Great Cake Eaters'? Damn. That was a backwards step in the evolutionary process. We left Shroomheads in the dust. Welcome to the new age of Vermheads.

VvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVv

I ONLY STUDY legal, edible mushrooms and mycology. Any mention of any other kind of mushroom is purely fictional, and pictures are ripped from the internet. I do not grow mushrooms!


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Offlinephantasm
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Registered: 09/10/04
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Re: G2G transfers...an endless cycle? [Re: spliffmasta]
    #3491421 - 12/13/04 03:30 PM (12 years, 6 months ago)

^^^^ good question i was just about to ask the same thing because i have used 1 pint jar for 10 pint jar G2G and they all turned out about the same

-mike


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Only God Grows Amanita Muscaria


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Offlinemushroommark
Earning mybluethumb

Registered: 10/01/04
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Re: G2G transfers...an endless cycle? [Re: phantasm]
    #3494234 - 12/13/04 10:43 PM (12 years, 6 months ago)

Yeah, spliff, I see what you mean and was just thinkin the same thing in class today. How many jars can you G2G with a quart:
say 1 qt goes to 20 then with each of those 20 you G2G 20 more so now you have 400 qt and each of those 400 G2G 20 more...and on and on exponentially

I don't see how generations comes in to play without birthing... but I am new to all this... hmmmm

I read this post again and realized I kinda repeated everyone else. whoops, but I wanted to put this back to the top, I'm very curious


Edited by mushroommark (12/13/04 10:46 PM)


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Invisibleagar
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Re: G2G transfers...an endless cycle? [Re: mushroommark]
    #3494470 - 12/13/04 11:22 PM (12 years, 6 months ago)

IMHO, MASTER GRAIN JAR, is from spores. Generation (G) 1, is G2G from Master Jar. G2 is G2G from G1, G3 is G2G from G2, so on & so forth.

IMHO, Agar MASTER PLATE is from spores (Or, tissue = clone). Agar wedge or slurry transfer to grain spawn is G1. G2G from that is G2, so on & so forth.

Tissue (clone) to agar plate is Master Plate. Agar wedge slurry out of Master Plate into grain jar is G1. A week later, agar wedge slurry from same Master Plate is still G1.

Consider this. G2G is sort of like a pregnancy. The myc is colonizing, RIGHT.

If you kept a embryo in a womb, then when it was near birth, transferred it to a another womb, so on & so forth, multiples of times.

DO YOU THINK THE BABY WOULD BE NORMAL, when it is born?

I don?t think so. I don?t G2G past G3. Otherwise, defects begin to show up.

I know, because starting out, I used to stretch G2G a lot further.

Below is example of about G7. Notice the GNARLY fruits.



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OfflineFerment
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Re: G2G transfers...an endless cycle? [Re: agar]
    #3494809 - 12/14/04 12:25 AM (12 years, 6 months ago)

heh, agar's upside down mushroom = teh rox. (right side of pic, in the back)


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Invisibleagar
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Re: G2G transfers...an endless cycle? [Re: Ferment]
    #3494850 - 12/14/04 12:30 AM (12 years, 6 months ago)

Quote:

Ferment said:
heh, agar's upside down mushroom = teh rox. (right side of pic, in the back)




:eek:


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Offlineoceansize
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Registered: 08/31/04
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Re: G2G transfers...an endless cycle? [Re: agar]
    #3494936 - 12/14/04 12:50 AM (12 years, 6 months ago)

ehh agar those are fucking gross :smile:

that last phallus looking thing... ughhh


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"And we should consider every day lost on which we have not danced at least once. And we should call every truth false which was not accompanied by at least one laugh." - Friedrich Nietzsche



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InvisibleADoobie
AdoobieShroomery
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Registered: 11/25/04
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Loc: I moved!
Re: G2G transfers...an endless cycle? [Re: oceansize]
    #3496358 - 12/14/04 10:57 AM (12 years, 6 months ago)

lol. Thanks agar. That clears it up :smile:

*gags*


--------------------
ADoobie said:
I know what strikes me as odd about this. It is totally unnatural. The whole idea is just totally backwards.

So are historians going to look back at 'The Great Cake Eaters'? Damn. That was a backwards step in the evolutionary process. We left Shroomheads in the dust. Welcome to the new age of Vermheads.

VvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVv

I ONLY STUDY legal, edible mushrooms and mycology. Any mention of any other kind of mushroom is purely fictional, and pictures are ripped from the internet. I do not grow mushrooms!


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InvisibleArp
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Re: G2G transfers...an endless cycle? [Re: agar]
    #3497708 - 12/14/04 04:19 PM (12 years, 6 months ago)

Quote:

agar said:
Quote:

Ferment said:
heh, agar's upside down mushroom = teh rox. (right side of pic, in the back)




:eek:




Oh poor poor mushroom :frown:


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Offlinemushroommark
Earning mybluethumb

Registered: 10/01/04
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Re: G2G transfers...an endless cycle? [Re: Arp]
    #3510365 - 12/16/04 08:33 PM (12 years, 6 months ago)

Right on agar, that was a very helpful!! I love when people answer from personal experience instead of just something they read.


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